. "Antifungal susceptibility testing by flow cytometry:is it the future?" . "RIV/00216208:11150/06:00005152!RIV08-MSM-11150___" . "The current increase in the number and significance of fungal infections, the expanding armamentarium of antifungal agents, and the emergence of the problem of antifungal drug resistance have been intensifying the importance of antifungal susceptibility testing. The CLSI in US a AFST-EUCAST published standard methodologies in order to achieve higher reproducibility and allow direct inter-laboratory comparison of the susceptibility results.Over the last 15 years, successful applications of flow cytometric techniques to AST of both yeast and moulds have been reported. These techniques are based on the analysis of a great number of fungal cells individually and frequently rely on short incubation times of no more than a few hours. Considering these attributes, flow cytometry (FC) seems to have the potential to achieve clinical usefulness in the near future. The collection of data on the reproducibility of the results and on the correlation with clinical outcomes has barely started, however."@en . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "Mycoses" . "0933-7407" . "Buchta, Vladim\u00EDr" . . "261;273" . "[26D6CC760DA2]" . "Antifungal susceptibility testing by flow cytometry:is it the future?"@en . "Antifungal; susceptibility; testing; cytometry; future"@en . . . . . "11150" . "465522" . . "The current increase in the number and significance of fungal infections, the expanding armamentarium of antifungal agents, and the emergence of the problem of antifungal drug resistance have been intensifying the importance of antifungal susceptibility testing. The CLSI in US a AFST-EUCAST published standard methodologies in order to achieve higher reproducibility and allow direct inter-laboratory comparison of the susceptibility results.Over the last 15 years, successful applications of flow cytometric techniques to AST of both yeast and moulds have been reported. These techniques are based on the analysis of a great number of fungal cells individually and frequently rely on short incubation times of no more than a few hours. Considering these attributes, flow cytometry (FC) seems to have the potential to achieve clinical usefulness in the near future. The collection of data on the reproducibility of the results and on the correlation with clinical outcomes has barely started, however." . "V sou\u010Dasn\u00E9 dob\u011B se zvy\u0161uje po\u010Det mykotick\u00FDch infekc\u00ED a v souvislosti s expanduj\u00EDc\u00EDm po\u010Dtem antimykotik tak\u00E9 stoup\u00E1 probl\u00E9m rezistence a t\u00EDm i nar\u016Fst\u00E1 d\u016Fle\u017Eitost testov\u00E1n\u00ED antifung\u00E1ln\u00ED citlivosti (AST). CLSI v USA a AFST-EUCAST uve\u0159ejnily standardn\u00ED metodiky, aby bylo mo\u017Eno dos\u00E1hnout vy\u0161\u0161\u00ED reproducibility a aby bylo mo\u017Eno prov\u00E1d\u011Bt p\u0159\u00EDm\u00E9 mezilaboratorn\u00ED porovn\u00E1v\u00E1n\u00ED v\u00FDsledk\u016F. V uplynul\u00FDch 15 letech byly pops\u00E1ny \u00FAsp\u011B\u0161n\u00E9 pokusy aplikovat pr\u016Ftokovou cytometrii v p\u0159\u00EDpad\u011B testov\u00E1n\u00ED AST kvasinek a pl\u00EDsn\u00ED. Tyto techniky jsou zalo\u017Eeny na anal\u00FDze velk\u00E9ho po\u010Dtu jednotliv\u00FDch bun\u011Bk a \u010Dasto pot\u0159ebuj\u00ED pouze kr\u00E1tk\u00E9 inkuba\u010Dn\u00ED \u010Dasy. Pr\u016Ftokov\u00E1 cytometrie se tedy zd\u00E1 b\u00FDt vhodn\u00FDm p\u0159\u00EDstupem v t\u00E9to oblasti do budoucna. Sb\u011Br \u00FAdaj\u016F a jejich korelace s klinick\u00FDmi v\u00FDsledky v\u0161ak teprve za\u010Dala. Praktick\u00E1 validace pokusn\u00FDch metod tedy nebude ud\u011Blena d\u0159\u00EDve ne\u017E bude k dispozici dostate\u010Dn\u00E1 z\u00E1soba \u00FAdaj\u016F \u0159e\u0161\u00EDc\u00EDch tyto ot\u00E1zky."@cs . . . "Testov\u00E1n\u00ED antifung\u00E1ln\u00ED aktivity pomoc\u00ED pr\u016Ftokov\u00E9 cytometrie"@cs . . . "1"^^ . . "2"^^ . . "Testov\u00E1n\u00ED antifung\u00E1ln\u00ED aktivity pomoc\u00ED pr\u016Ftokov\u00E9 cytometrie"@cs . "13"^^ . "RIV/00216208:11150/06:00005152" . "Antifungal susceptibility testing by flow cytometry:is it the future?" . "Antifungal susceptibility testing by flow cytometry:is it the future?"@en . "S, V" . "4" . . "49" . . . .