. "4" . "345;347" . . . "\u010Cerm\u00E1kov\u00E1, Zuzana" . "527659" . "Laboratory Diagnosis of Leptospirosis" . . "S" . . "[BBA62AD10D4B]" . "RIV/00216208:11150/05:00004932" . "Laboratorn\u00ED diagn\u00F3za leptospir\u00F3zy"@cs . "Ry\u0161kov\u00E1, Olga" . "3"^^ . "11150" . "Folia Microbiologica" . . "RIV/00216208:11150/05:00004932!RIV08-MSM-11150___" . . "Laboratory Diagnosis of Leptospirosis"@en . "Percentage of serological positivity examined in 4205 blood sera by serological method microscopic agglutination test (MAT) on the hinterland territory of our laboratory (East Bohemia; 1999-2003) was 0.38-4.7 %. By the PCR method for detection of DNA of pathogenic leptospires (L. interrogans, L. borgpetersenii and L. kirschneri) from 57 samples of different biological materials from patients with fever of unknown etiology positive results were obtained in 4 specimens (7 %; 3 samples of urine and 1 sample of blood). This method was shown to distinguish between pathogenic and nonpathogenic strains and can detect 2.5-10 cells per mL of biological material. As an important presumption of successful detection of pathogenic leptospires a correct collecting of blood, urine samples or liquor is required before starting antibody therapy."@en . . "Laboratory Diagnosis of Leptospirosis" . . "Laboratorn\u00ED diagn\u00F3za leptospir\u00F3zy"@cs . "Procento s\u00E9rologick\u00E9 pozitivity vy\u0161et\u0159ovan\u00E9 u 4205 lidsk\u00FDch s\u00E9r pomoc\u00ED mikroskopick\u00E9ho aglutina\u010Dn\u00EDho testu (MAT) v teritori\u00E1ln\u00ED oblasti na\u0161\u00ED laborato\u0159e (V\u00FDchodn\u00ED \u010Cechy; 1999-2003) bylo 0,38-4,7 %. Detekce DNA patogenn\u00EDch leptospir (L. interrogans, L. borgpetersenii a L. kirschneri) pomoc\u00ED PCR byla provedena u 57 vzork\u016F r\u016Fzn\u00FDch biologick\u00FDch materi\u00E1l\u016F od pacient\u016F trp\u00EDc\u00EDch hore\u010Dkou nezn\u00E1m\u00E9ho p\u016Fvodu a ve 4 vzorc\u00EDch (7 %; 3 vzorky mo\u010Di a 1 vzorek krve) byly z\u00EDsk\u00E1ny pozitivn\u00ED \u00FAdaje. Tato metoda je schopn\u00E1 odli\u0161it patogenn\u00ED a nepatogenn\u00ED kmeny a je schopn\u00E1 zachytit 2,5-10 bun\u011Bk na 1 ml biologick\u00E9ho materi\u00E1lu. D\u016Fle\u017Eitou presumpc\u00ED \u00FAsp\u011B\u0161n\u00E9 detekce patogenn\u00EDch leptospir je nutnost spr\u00E1vn\u011B odeb\u00EDrat krevn\u00ED a mo\u010Dov\u00E9 vzorky a likvor p\u0159ed zah\u00E1jen\u00EDm terapie protil\u00E1tkami. PCR metoda je jasn\u011B v\u00FDhodn\u011Bj\u0161\u00ED ne\u017E ostatn\u00ED diagnostick\u00E9 metody jako nap\u0159. MAT, kter\u00E1 detekuje p\u0159\u00EDtomnost protil\u00E1tek v krvi naka\u017Een\u00E9ho \u010Dlov\u011Bka, kter\u00E9 se v\u0161ak objev\u00ED a\u017E mnoho dn\u00ED po zah\u00E1jen\u00ED infekce."@cs . . "0015-5632" . . "Percentage of serological positivity examined in 4205 blood sera by serological method microscopic agglutination test (MAT) on the hinterland territory of our laboratory (East Bohemia; 1999-2003) was 0.38-4.7 %. By the PCR method for detection of DNA of pathogenic leptospires (L. interrogans, L. borgpetersenii and L. kirschneri) from 57 samples of different biological materials from patients with fever of unknown etiology positive results were obtained in 4 specimens (7 %; 3 samples of urine and 1 sample of blood). This method was shown to distinguish between pathogenic and nonpathogenic strains and can detect 2.5-10 cells per mL of biological material. As an important presumption of successful detection of pathogenic leptospires a correct collecting of blood, urine samples or liquor is required before starting antibody therapy." . "CZ - \u010Cesk\u00E1 republika" . "3"^^ . . "Laboratory; Diagnosis; Leptospirosis"@en . "50" . "2"^^ . "Laboratory Diagnosis of Leptospirosis"@en . . . .