"Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria"@en . "Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria" . "11150" . . "Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria"@en . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "0951-6433" . . . . . . . "BioFactors" . "RIV/00216208:11150/05:00003605!RIV06-MSM-11150___" . "552126" . . "Zinkem indukovan\u00E1 apopt\u00F3za u HEP-2 n\u00E1dorov\u00FDch bun\u011Bk: \u00FAloha oxida\u010Dn\u00EDho stresu a mitochondri\u00ED"@cs . "107;120" . . "Z(MSM0021620820)" . "Apopt\u00F3za indukovan\u00E1 s\u00EDranem zine\u010Dnat\u00FDm na bun\u011B\u010Dn\u00E9 linii HEP-2 byla sledov\u00E1na b\u011Bhem 96hodinov\u00E9 expozice. B\u011Bhem prvn\u00EDch 48 hodin zinek vstupoval do mitochondri\u00ED a vyvol\u00E1val tvorbu kysl\u00EDkov\u00FDch radik\u00E1l\u016F. U exponovan\u00FDch bun\u011Bk doch\u00E1zelo k m\u00EDrn\u00E9 aktivaci p53, sn\u00ED\u017Een\u00ED GSH a po\u0161kozen\u00ED mitochondr\u00E1ln\u00EDch funkc\u00ED. Aktivace apopt\u00F3zy byla charakterizov\u00E1na uvoln\u011Bn\u00EDm cytochromu C a AIF do cytoplazmy a j\u00E1dra. Pou\u017Eit\u00ED r\u016Fzn\u00FDch farmakologick\u00FDch inhibitor\u016F apopt\u00F3zy (antioxidanty NAC a koenzym Q, kasp\u00E1zov\u00E9 inhibitory z-DEVD-fmk a z-VAD-fmk, cyklosporin A a bonkgrekov\u00E1 kyselina) prok\u00E1zalo, \u017Ee zinek \u00FA\u010Dinkuje p\u0159\u00EDmo i nep\u0159\u00EDmo na mitochondrie a v\u00FDsledn\u00E1 apopt\u00F3za je na kasp\u00E1z\u00E1ch z\u00E1visl\u00E1 i nez\u00E1visl\u00E1."@cs . "23" . "3"^^ . "Induction of apoptosis by zinc sulfate was investigated during 96 h exposure on the cancer HEP-2 cell line. During 48 h of exposure, zinc translocated into mitochondria and stimulated production of reactive oxygen species (ROS), affected cellular GSH management and induced moderate activation of p53 and dissipation of mitochondrial membrane potential. In Zn-exposed cells, mitochondria released cytochrome c and AIF, whose translocation to the cytoplasm or the nucleus coincided with the activation of apoptosis. The use of various pharmacological inhibitors inhibiting particular apoptotic targets (antioxidants such as N-acetyl-cysteine and coenzyme Q, the caspase inhibitors z-DEVD-fmk and z-VAD-fmk, cyclosporin A and bonkgrekic acid) proved that Zn acts both directly and indirectly on mitochondria and observed apoptosis is executed by caspase-dependent and caspase-independent pathways."@en . . "Zinkem indukovan\u00E1 apopt\u00F3za u HEP-2 n\u00E1dorov\u00FDch bun\u011Bk: \u00FAloha oxida\u010Dn\u00EDho stresu a mitochondri\u00ED"@cs . "Rudolf, Emil" . . . . . . "14"^^ . "2" . "RIV/00216208:11150/05:00003605" . "Induction of apoptosis by zinc sulfate was investigated during 96 h exposure on the cancer HEP-2 cell line. During 48 h of exposure, zinc translocated into mitochondria and stimulated production of reactive oxygen species (ROS), affected cellular GSH management and induced moderate activation of p53 and dissipation of mitochondrial membrane potential. In Zn-exposed cells, mitochondria released cytochrome c and AIF, whose translocation to the cytoplasm or the nucleus coincided with the activation of apoptosis. The use of various pharmacological inhibitors inhibiting particular apoptotic targets (antioxidants such as N-acetyl-cysteine and coenzyme Q, the caspase inhibitors z-DEVD-fmk and z-VAD-fmk, cyclosporin A and bonkgrekic acid) proved that Zn acts both directly and indirectly on mitochondria and observed apoptosis is executed by caspase-dependent and caspase-independent pathways." . . "Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria" . "\u010Cervinka, Miroslav" . . "[94D844B69D7E]" . "Zinc; oxidative stress; energy metabolism; mitochondria; apoptosis"@en . "2"^^ .