"Acta Medica (Hradec Kr\u00E1lov\u00E9)" . "3"^^ . "The multidrug resistance and apoptosis evaluation in acute myeloid leukemia cells after the in vitro doxorubicin treatment" . . . "Novosad, Jakub" . . "CZ - \u010Cesk\u00E1 republika" . "RIV/00216208:11150/04:00004429!RIV08-MSM-11150___" . "1211-4286" . . "181;185" . "11150" . "S" . . "multidrug; resistance; apoptosis; evaluation; acute; myeloid; leukemia; cells; after; vitro; doxorubicin; treatment"@en . "5"^^ . . "[2A15F8ED68E6]" . . "The multidrug resistance and apoptosis evaluation in acute myeloid leukemia cells after the in vitro doxorubicin treatment"@en . . . . . "3" . "Hodnocen\u00ED mnoho\u010Detn\u00E9 l\u00E9kov\u00E9 rezistence u bun\u011Bk akutn\u00ED myeloidn\u00ED leuk\u00E9mie po o\u0161et\u0159en\u00ED doxorubicinem in vitro"@cs . . "Selh\u00E1n\u00ED apopt\u00F3zy p\u0159i l\u00E9\u010Db\u011B hemoblast\u00F3zy cytostatiky je jednou z p\u0159\u00ED\u010Din chemorezistence. Zaj\u00EDmali jsme se o vztah apopt\u00F3zy AML bun\u011Bk po pod\u00E1n\u00ED doxorubicinu a imunofenotypem, vybran\u00FDmi klinick\u00FDmi a laboratorn\u00EDmi parametry a expres\u00ED protein\u016F P-gp, MRP, LRP, Bcl-2 a Bax. V\u0161echny anal\u00FDzy byly hodnoceny metodou pr\u016Ftokov\u00E9 cytometrie. K detekci apoptotick\u00FDch bun\u011Bk ve vzorku byly pou\u017Eity 3 metody: annexin test, TUNEL (TdT-mediated dUTP nick end labeling) test a detekce kasp\u00E1zy 8. Po kultivaci bun\u011Bk byl z\u0159ejm\u00FD statistick\u00FD n\u00E1r\u016Fst apoptotick\u00FDch bun\u011Bk v kultu\u0159e. Byl tak\u00E9 pozorov\u00E1n vztah mezi in vitro blastickou reakc\u00ED AML bun\u011Bk a n\u011Bkter\u00FDmi klinick\u00FDmi parametry jako v\u011Bk pacienta, po\u010Det b\u00EDl\u00FDch krvinek a procento blastick\u00FDch bun\u011Bk"@cs . . "The multidrug resistance and apoptosis evaluation in acute myeloid leukemia cells after the in vitro doxorubicin treatment" . "Kopeck\u00FD, Otakar" . "Krejsek, Jan" . "The apoptosis failure in cytostatic treatment of haemoblastosis is one of the means of chemoresistance. We were interested in the relationship of the after-doxorubicin-treatment-AML cells apoptosis and the immunophenotype, selected clinical and laboratory parameters, and also the P-gp, MRP, LRP, Bcl-2, Bax proteins expression. All analysis were evaluated with the flow cytometry method. To detect apoptotic cells in the sample, we used three methods: annexin test, TUNEL (TdT-mediated dUTP nick end labeling) test, and caspase 8 detection. After the cell cultivation the statisticaly important increase of apoptotic cells in the culture was apparent. The relation between the AML blast in vitro reaction and some clinical parameters such as the age of patient, white blood cell count, and blast percentage was also observed. : AML; Apoptosis; Multidrug resistance; Flow cytometry"@en . . "Hodnocen\u00ED mnoho\u010Detn\u00E9 l\u00E9kov\u00E9 rezistence u bun\u011Bk akutn\u00ED myeloidn\u00ED leuk\u00E9mie po o\u0161et\u0159en\u00ED doxorubicinem in vitro"@cs . . . "6"^^ . . . . . "The apoptosis failure in cytostatic treatment of haemoblastosis is one of the means of chemoresistance. We were interested in the relationship of the after-doxorubicin-treatment-AML cells apoptosis and the immunophenotype, selected clinical and laboratory parameters, and also the P-gp, MRP, LRP, Bcl-2, Bax proteins expression. All analysis were evaluated with the flow cytometry method. To detect apoptotic cells in the sample, we used three methods: annexin test, TUNEL (TdT-mediated dUTP nick end labeling) test, and caspase 8 detection. After the cell cultivation the statisticaly important increase of apoptotic cells in the culture was apparent. The relation between the AML blast in vitro reaction and some clinical parameters such as the age of patient, white blood cell count, and blast percentage was also observed. : AML; Apoptosis; Multidrug resistance; Flow cytometry" . "RIV/00216208:11150/04:00004429" . "574980" . . . . "The multidrug resistance and apoptosis evaluation in acute myeloid leukemia cells after the in vitro doxorubicin treatment"@en . . "47" . .