"We evaluated the effects of proteasome inhibitors - a peptide aldehyde MG132, and two vinylsulfone inhibitors ZL3VS and AdaAhx3L3VS - on protein metabolism in sepsis. Sepsis was induced in rats by caecal ligation and puncture. Parameters of protein metabolism were measured in incubated rat skeletal muscles. Total proteolysis was determined according to the rates of tyrosine release into the medium during incubation. The ratesof protein synthesis and leucine oxidation were evaluated by incubating muslces in medium containing L-/1-14C/leucine. In the septic muscles, all three inhibitors testedhad no effect on protein synthesis.MG132 decreased proteolysis by more then 50%, AdaAhx3L3VS by 20%, while the effect of ZL3VS was not significant. We conclude, that MG132 and AdaAhx3L3VS reversed at least partly the protein catabolism in sepsis, as they decreased the proteolysis and did not change the protein synthesis." . "V pr\u00E1ci je posouzen \u00FA\u010Dinek inhibitor\u016F proteasom\u016F-peptidov\u00E9ho aldehydu MG132,2 peptidick\u00FDch vinylsulfon\u016F ZL3VS a AdaAhx3L3VS na metabolismus protein\u016F u sepse. Sepse byla indukov\u00E1na ligac\u00ED a n\u00E1slednou punkc\u00ED slep\u00E9ho st\u0159eva. Parametry metabolismu protein\u016F byly stanoveny na modelu inkubovan\u00E9ho kostern\u00EDho svalu. Proteol\u00FDza byla vypo\u010Dtena na z\u00E1klad\u011B uvoln\u011Bn\u00ED tyrosinu do inkuba\u010Dn\u00EDho m\u00E9dia. Proteosynt\u00E9za a oxidace leucinu pomoc\u00ED L-/1-14C/leucinu. Inhibitory proteasom\u016F nem\u011Bly vliv na proteosynt\u00E9zu. MG132 sn\u00ED\u017Eil proteol\u00FDzu o v\u00EDce ne\u017E 50 %, AdaAhx3L3VS o 20 % a \u00FA\u010Dinek ZL3VS byl nesignifikantn\u00ED. Z\u00E1v\u011Brem je konstatov\u00E1no, \u017Ee MG132 a AdaAHx3L3VS eliminovaly proteokatabolismus indukovan\u00FD seps\u00ED. Mechanismem bylo sn\u00ED\u017Een\u00ED proteol\u00FDzy a ne stimulace proteosynt\u00E9zy."@cs . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "proteasome inhibitors;protein metabolism;sepsis"@en . . . . . . "5"^^ . "0959-9673" . "363;371" . "RIV/00216208:11150/04:00002083" . "RIV/00216208:11150/04:00002083!RIV/2005/GA0/111505/N" . "We evaluated the effects of proteasome inhibitors - a peptide aldehyde MG132, and two vinylsulfone inhibitors ZL3VS and AdaAhx3L3VS - on protein metabolism in sepsis. Sepsis was induced in rats by caecal ligation and puncture. Parameters of protein metabolism were measured in incubated rat skeletal muscles. Total proteolysis was determined according to the rates of tyrosine release into the medium during incubation. The ratesof protein synthesis and leucine oxidation were evaluated by incubating muslces in medium containing L-/1-14C/leucine. In the septic muscles, all three inhibitors testedhad no effect on protein synthesis.MG132 decreased proteolysis by more then 50%, AdaAhx3L3VS by 20%, while the effect of ZL3VS was not significant. We conclude, that MG132 and AdaAhx3L3VS reversed at least partly the protein catabolism in sepsis, as they decreased the proteolysis and did not change the protein synthesis."@en . "P(GA303/03/1512)" . . . "Vliv inhibitor\u016F proteasomu MG132, ZL3VS a AdaAhX3-L3VS na metabolismus protein\u016F u potkan\u016F v sepsi"@cs . "\u0160afr\u00E1nek, Roman" . "Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats"@en . "Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats"@en . . "1" . "Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats" . "International Journal of Experimental Pathology" . . . "Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats" . "Hole\u010Dek, Milan" . . "85" . "562192" . "[F0E349A1DEFD]" . . . "Vliv inhibitor\u016F proteasomu MG132, ZL3VS a AdaAhX3-L3VS na metabolismus protein\u016F u potkan\u016F v sepsi"@cs . . "9"^^ . "2"^^ . "11150" .