"Switch from a dominant Th1-associated immune profile during the pre-diabetic phase in favour of a temporary increase of a Th3-associated and inflammatory immune profile at the onset of type 1 diabetes"@en . "This study aims to confirm previous observations of a dominant T-helper (Th)1-like profile during the period close to onset of disease. Peripheral blood mononuclear cells were collected 4 days and 1 and 2 years after diagnosis of T1D children, from healthy children carrying the human leukocyte antigen-risk genes and from high-risk children (ICA >= 20 IJDF units). Peripheral blood mononuclear cells were stimulated by diabetes associated autoantigens and cytokines and chemokines were detected in cell-culture supernatants by protein microarray in relation to clinical outcome (C-peptide). High-risk children showed a dominant Th1-associated profile with high spontaneous and GAD(65)-induced secretion. In contrast, newly diagnosed T1D children showed a pronounced Th3-associated cytokine profile as well as a burst of inflammatory cytokines and chemokines. The Th1 dominance typical of prediabetes phase, however, diminished by the time of T1D diagnose." . "Type 1 diabetes; high-risk children; T-helper cells; T-regulatory cells; cytokines; chemokines; monocyte chemoattractant protein-1; glutamic-acid decarboxylase; chronic autoimmune-disease; blood mononuclear-cells; ifn-gamma; high-risk; nod mice; t-cells; cytokine production; regulatory-cells/"@en . . . "Ryden, A." . . "Diabetes-Metabolism Research and Reviews" . . "1520-7552" . "This study aims to confirm previous observations of a dominant T-helper (Th)1-like profile during the period close to onset of disease. Peripheral blood mononuclear cells were collected 4 days and 1 and 2 years after diagnosis of T1D children, from healthy children carrying the human leukocyte antigen-risk genes and from high-risk children (ICA >= 20 IJDF units). Peripheral blood mononuclear cells were stimulated by diabetes associated autoantigens and cytokines and chemokines were detected in cell-culture supernatants by protein microarray in relation to clinical outcome (C-peptide). High-risk children showed a dominant Th1-associated profile with high spontaneous and GAD(65)-induced secretion. In contrast, newly diagnosed T1D children showed a pronounced Th3-associated cytokine profile as well as a burst of inflammatory cytokines and chemokines. The Th1 dominance typical of prediabetes phase, however, diminished by the time of T1D diagnose."@en . "344943" . . "Switch from a dominant Th1-associated immune profile during the pre-diabetic phase in favour of a temporary increase of a Th3-associated and inflammatory immune profile at the onset of type 1 diabetes" . . . . "[001BA8A67F90]" . . "4" . . . "Faresjo, M." . . "25" . . "11130" . . . "Durilov\u00E1, Marianna" . . "000266347200008" . "Z(MZ0FNM2005)" . . . . "Switch from a dominant Th1-associated immune profile during the pre-diabetic phase in favour of a temporary increase of a Th3-associated and inflammatory immune profile at the onset of type 1 diabetes"@en . . "RIV/00216208:11130/09:5393!RIV10-MZ0-11130___" . . "9"^^ . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "\u0160techov\u00E1, Kate\u0159ina" . . . . . "2"^^ . "4"^^ . "RIV/00216208:11130/09:5393" . "Switch from a dominant Th1-associated immune profile during the pre-diabetic phase in favour of a temporary increase of a Th3-associated and inflammatory immune profile at the onset of type 1 diabetes" . . . .