. "1210-7913" . "2"^^ . . "11"^^ . . "Enterohemorrhagic Escherichia coli as the cause of diarrhea in the Czech Republic, 1965\u20132013"@en . "[6EE934E24337]" . . "14712" . "1"^^ . . "RIV/00216208:11120/14:43908575" . . "Enterohemoragick\u00E9 Escherichia coli jako p\u016Fvodci pr\u016Fjmu v \u010Cesk\u00E9 republice, 1965-2013"@cs . . "CZ - \u010Cesk\u00E1 republika" . "Czech Republic; diarrhea; genotype; Shiga toxin; Enterohaemorrhagic Escherichia coli"@en . "3" . . . "Enterohemoragick\u00E9 Escherichia coli jako p\u016Fvodci pr\u016Fjmu v \u010Cesk\u00E9 republice, 1965-2013"@cs . . "Enterohemoragick\u00E9 Escherichia coli jako p\u016Fvodci pr\u016Fjmu v \u010Cesk\u00E9 republice, 1965-2013" . . "63" . "Marejkov\u00E1, Monika" . . "V t\u00E9to studii jsme analyzovali s\u00E9rotypy, stx genotypy a faktory virulence kmen\u016F EHEC izolovan\u00FDch v letech 1965\u20132013 od pacient\u016F s pr\u016Fjmem, krvav\u00FDm pr\u016Fjmem a jejich rodinn\u00FDch kontakt\u016F. D\u00E1le jsme charakterizovali diagnosticky d\u016Fle\u017Eit\u00E9 fenotypy kmen\u016F EHEC, jejich citlivost k antimikrobi\u00E1ln\u00EDm agens, sezonnost a distribuci podle kraj\u016F. Materi\u00E1l a metody: Kmeny od pacient\u016F byly zasl\u00E1ny jako izol\u00E1ty E. coli ur\u010Den\u00E9 s\u00E9roskupiny do NRL pro E. coli a shigely k pr\u016Fkazu Stx. V souvislosti s epidemiologick\u00FDm \u0161et\u0159en\u00EDm byly zasl\u00E1ny do NRL vzorky hum\u00E1nn\u00EDho a nonhum\u00E1nn\u00EDho p\u016Fvodu. S\u00E9rotypy byly ur\u010Deny konven\u010Dn\u00ED a molekul\u00E1rn\u00ED s\u00E9rotypizac\u00ED, stx genotypy a non-stx faktory virulence pomoc\u00ED PCR, a fenotypy a antimikrobi\u00E1ln\u00ED citlivost pomoc\u00ED standardn\u00EDch metod. Epidemiologick\u00E1 souvislost izol\u00E1t\u016F od zv\u00ED\u0159ete a pacienta byla potvrzena metodou pulzn\u00ED gelov\u00E9 elektrofor\u00E9zy (PFGE). Z\u00E1v\u011Br: EHEC se vyskytuj\u00ED jako p\u016Fvodci pr\u016Fjmu a krvav\u00E9ho pr\u016Fjmu v \u010CR. Pouze systematick\u00FD screening t\u011Bchto patogen\u016F ve stolic\u00EDch pacient\u016F v\u0161ak umo\u017En\u00ED odhalit jejich skute\u010Dn\u00FD pod\u00EDl na etiologii pr\u016Fjmov\u00FDch onemocn\u011Bn\u00ED (a rovn\u011B\u017E HUS) v \u010CR, a za\u0159adit tak tato data do evropsk\u00E9ho kontextu. Onemocn\u011Bn\u00ED vyvolan\u00E1 EHEC jsou hl\u00E1\u0161ena do Evropsk\u00E9ho centra pro prevenci a kontrolu nemoc\u00ED (ECDC) v r\u00E1mci surveillance Food and Waterborne Diseases." . "Enterohemoragick\u00E9 Escherichia coli jako p\u016Fvodci pr\u016Fjmu v \u010Cesk\u00E9 republice, 1965-2013" . "Therefore, this study analyzed the serotypes, stx genotypes, and virulence factors in EHEC strains isolated in 1965\u20132013 from patients with diarrhea or bloody diarrhea and their family contacts. In addition, we characterized diagnostically relevant phenotypes of EHEC strains, their antimicrobial susceptibility, seasonal trends, and distribution by administrative region. Material and methods: Serogrouped E. coli isolates from patients were referred to the National Reference Laboratory (NRL) for E. coli and Shigella for the detection of Stx. Specimens of both human and non-human origin were referred to the NRL for epidemiological investigation. Serotyping was performed by conventional and molecular methods, PCR was applied to stx genotyping and identification of non-stx virulence factors, and standard methods were used for phenotypic analysis and antimicrobial susceptibility testing. The epidemiological link between the human and animal isolates was confirmed using pulsed-field gel electrophoresis (PFGE). Conclusion: EHEC strains cause diarrhea and bloody diarrhea in the Czech Republic. Nevertheless, only a systematic screening of the stool from patients with diarrhea can make it possible to elucidate their actual role in the etiology of diarrheal diseases (as well as HUS) in the Czech Republic and to consider the data in the European context. EHEC cases are reported to the European Centre for Disease Prevention and Control (ECDC) within the Food and Waterborne Diseases Surveillance Network."@en . "Enterohemorrhagic Escherichia coli as the cause of diarrhea in the Czech Republic, 1965\u20132013"@en . "11120" . . "V t\u00E9to studii jsme analyzovali s\u00E9rotypy, stx genotypy a faktory virulence kmen\u016F EHEC izolovan\u00FDch v letech 1965\u20132013 od pacient\u016F s pr\u016Fjmem, krvav\u00FDm pr\u016Fjmem a jejich rodinn\u00FDch kontakt\u016F. D\u00E1le jsme charakterizovali diagnosticky d\u016Fle\u017Eit\u00E9 fenotypy kmen\u016F EHEC, jejich citlivost k antimikrobi\u00E1ln\u00EDm agens, sezonnost a distribuci podle kraj\u016F. Materi\u00E1l a metody: Kmeny od pacient\u016F byly zasl\u00E1ny jako izol\u00E1ty E. coli ur\u010Den\u00E9 s\u00E9roskupiny do NRL pro E. coli a shigely k pr\u016Fkazu Stx. V souvislosti s epidemiologick\u00FDm \u0161et\u0159en\u00EDm byly zasl\u00E1ny do NRL vzorky hum\u00E1nn\u00EDho a nonhum\u00E1nn\u00EDho p\u016Fvodu. S\u00E9rotypy byly ur\u010Deny konven\u010Dn\u00ED a molekul\u00E1rn\u00ED s\u00E9rotypizac\u00ED, stx genotypy a non-stx faktory virulence pomoc\u00ED PCR, a fenotypy a antimikrobi\u00E1ln\u00ED citlivost pomoc\u00ED standardn\u00EDch metod. Epidemiologick\u00E1 souvislost izol\u00E1t\u016F od zv\u00ED\u0159ete a pacienta byla potvrzena metodou pulzn\u00ED gelov\u00E9 elektrofor\u00E9zy (PFGE). Z\u00E1v\u011Br: EHEC se vyskytuj\u00ED jako p\u016Fvodci pr\u016Fjmu a krvav\u00E9ho pr\u016Fjmu v \u010CR. Pouze systematick\u00FD screening t\u011Bchto patogen\u016F ve stolic\u00EDch pacient\u016F v\u0161ak umo\u017En\u00ED odhalit jejich skute\u010Dn\u00FD pod\u00EDl na etiologii pr\u016Fjmov\u00FDch onemocn\u011Bn\u00ED (a rovn\u011B\u017E HUS) v \u010CR, a za\u0159adit tak tato data do evropsk\u00E9ho kontextu. Onemocn\u011Bn\u00ED vyvolan\u00E1 EHEC jsou hl\u00E1\u0161ena do Evropsk\u00E9ho centra pro prevenci a kontrolu nemoc\u00ED (ECDC) v r\u00E1mci surveillance Food and Waterborne Diseases."@cs . . "Epidemiologie, mikrobiologie, imunologie" . . . "S" . "RIV/00216208:11120/14:43908575!RIV15-MSM-11120___" .