"N\u011Bmcov\u00E1-F\u00FCrstov\u00E1, Vlasta" . "RIV/00216208:11120/11:00003234!RIV12-MSM-11120___" . "Cellular Physiology and Biochemistry" . "5" . . . "http://dx.doi.org/10.1159/000329954" . "10.1159/000329954" . . "Inhibitory effect of unsaturated fatty acids on saturated fatty acid-induced apoptosis in human pancreatic beta-Cells: Activation of caspases and ER stress induction" . . . "27" . . "CH - \u0160v\u00FDcarsk\u00E1 konfederace" . . "14"^^ . "Inhibitory effect of unsaturated fatty acids on saturated fatty acid-induced apoptosis in human pancreatic beta-Cells: Activation of caspases and ER stress induction"@en . "Inhibitory effect of unsaturated fatty acids on saturated fatty acid-induced apoptosis in human pancreatic beta-Cells: Activation of caspases and ER stress induction" . . "000291754000012" . . "James, Roger F. L." . . . "11120" . "S, Z(MSM0021620814)" . "Inhibitory effect of unsaturated fatty acids on saturated fatty acid-induced apoptosis in human pancreatic beta-Cells: Activation of caspases and ER stress induction"@en . "1015-8987" . . . . "In this study we have tested the effect of unsaturated fatty acids on the proapoptotic effects of saturated fatty acids in the human pancreatic beta-cells NES2Y. We found that unsaturated palmitoleic and oleic acid at a concentration of 0.2 mM and higher are able to completely inhibit the proapoptotic effect of their counterpart saturated palmitic and stearic acid at a concentration of 1 mM. Apoptosis induced by stearic acid was associated with significant activation of caspase-6, -7, -9, -2 and -8, but not with significant activation of caspase-3. The activation of caspases was blocked by coincubation with oleic acid. Stearic acid treatment was not associated with a significant change in mitochondrial membrane potential, reactive oxygen species level and with cytochrome c release from mitochondria. Furthermore, stearic acid treatment was not associated with changes in p21(WAF1/CIP1), PIDD, Fas receptor and Fas ligand expression. However, we detected endoplasmic reticulum (ER) stress markers, i.e. a s" . "Kov\u00E1\u0159, Jan" . . "[383B06F07C4E]" . . "2"^^ . . "204858" . "In this study we have tested the effect of unsaturated fatty acids on the proapoptotic effects of saturated fatty acids in the human pancreatic beta-cells NES2Y. We found that unsaturated palmitoleic and oleic acid at a concentration of 0.2 mM and higher are able to completely inhibit the proapoptotic effect of their counterpart saturated palmitic and stearic acid at a concentration of 1 mM. Apoptosis induced by stearic acid was associated with significant activation of caspase-6, -7, -9, -2 and -8, but not with significant activation of caspase-3. The activation of caspases was blocked by coincubation with oleic acid. Stearic acid treatment was not associated with a significant change in mitochondrial membrane potential, reactive oxygen species level and with cytochrome c release from mitochondria. Furthermore, stearic acid treatment was not associated with changes in p21(WAF1/CIP1), PIDD, Fas receptor and Fas ligand expression. However, we detected endoplasmic reticulum (ER) stress markers, i.e. a s"@en . . "3"^^ . "fatty acids; apoptosis; beta-cell; caspases; endoplasmic reticulum stress"@en . . . "RIV/00216208:11120/11:00003234" .