. . . . . "Circulating tumor cells (CTCs) are potential precursors of metastasis. They are also of use in diagnosing malignancy and for prognostic purposes. Our laboratory has previously isolated CTCs from orthotopic nude mouse models of human prostate cancer cells where the PC-3 cancer cells express green fluorescent protein (GFP). It was found that orthotopic tumors produced CTCs and not subcutaneous tumors, which may explain why orthotopic tumors metastasize and subcutaneous tumors do not. However, in this previous study, CTCs were observed only after culture. In the present study, using the GFP-expressing PC-3 orthotopic model and immunomagnetic beads coated with anti-epithelial cell adhesion molecule (EpCAM) and anti-prostate specific membrane antigen (PSMA), GFP-expressing CTC were isolated within 15 minutes and were readily visualized by GFP fluorescence." . "Anticancer Research" . . . "190445" . . "Circulating Human Prostate Cancer Cells from an Orthotopic Mouse Model Rapidly Captured by Immunomagnetic Beads and Imaged by GFP Expression"@en . "Kolo\u0161tov\u00E1, Katar\u00EDna" . . "Circulating Human Prostate Cancer Cells from an Orthotopic Mouse Model Rapidly Captured by Immunomagnetic Beads and Imaged by GFP Expression" . . "Circulating Human Prostate Cancer Cells from an Orthotopic Mouse Model Rapidly Captured by Immunomagnetic Beads and Imaged by GFP Expression" . "GR - \u0158eck\u00E1 republika" . "GFP; prostate cancer; PC-3; orthotopic; circulating cancer cells; immunomagnetic beads; isolation; fluorescence; imaging; culture"@en . . . "RIV/00216208:11120/11:00003170!RIV12-MZ0-11120___" . "P(ME 831), P(ME10045), P(NS9976), Z(MSM0021620816)" . . . "Bobek, Vladim\u00EDr" . . "5"^^ . . . "Pint\u00E9rov\u00E1, Daniela" . "RIV/00216208:11120/11:00003170" . . . . "000291235300004" . "11120" . "Circulating Human Prostate Cancer Cells from an Orthotopic Mouse Model Rapidly Captured by Immunomagnetic Beads and Imaged by GFP Expression"@en . "3"^^ . . . "[9B41EC8E7D63]" . . . . "5" . . . "Circulating tumor cells (CTCs) are potential precursors of metastasis. They are also of use in diagnosing malignancy and for prognostic purposes. Our laboratory has previously isolated CTCs from orthotopic nude mouse models of human prostate cancer cells where the PC-3 cancer cells express green fluorescent protein (GFP). It was found that orthotopic tumors produced CTCs and not subcutaneous tumors, which may explain why orthotopic tumors metastasize and subcutaneous tumors do not. However, in this previous study, CTCs were observed only after culture. In the present study, using the GFP-expressing PC-3 orthotopic model and immunomagnetic beads coated with anti-epithelial cell adhesion molecule (EpCAM) and anti-prostate specific membrane antigen (PSMA), GFP-expressing CTC were isolated within 15 minutes and were readily visualized by GFP fluorescence."@en . "31" . "4"^^ . . . "0250-7005" .