. "CZ - \u010Cesk\u00E1 republika" . "9"^^ . . . "A Fraction of MCM 2 Proteins Remain Associated with Replication Foci During a Major Part of S Phase"@en . "1" . "1"^^ . "Ra\u0161ka, Otakar" . . "Folia Biologica" . "A Fraction of MCM 2 Proteins Remain Associated with Replication Foci During a Major Part of S Phase" . "A Fraction of MCM 2 Proteins Remain Associated with Replication Foci During a Major Part of S Phase"@en . "The essential role of MCM 2-7 proteins in the initiation of DNA replication in all eukaryotes is well known. Their role in replication elongation is supported by numerous studies, but there is still a knowledge gap in this respect. Even though biochemical studies have established an association of MCM proteins with replication forks, previous immunofluorescence studies in mammalian cells have suggested that MCM 2-7 proteins are displaced after replication initiation from sites of DNA replication. Therefore, we used a robust statistical method to more precisely analyse immunofluorescence localization of MCM 2 proteins with respect to the DNA replication foci. We show that despite the predominantly different localization of MCM 2 and replication signals, there is still a small but significant fraction of MCM 2 proteins that co-localize with DNA replication foci during most of S phase. The fluorescence localization of the MCM 2 proteins and DNA replication may thus reflect an active function of MCM 2 pro" . . "The essential role of MCM 2-7 proteins in the initiation of DNA replication in all eukaryotes is well known. Their role in replication elongation is supported by numerous studies, but there is still a knowledge gap in this respect. Even though biochemical studies have established an association of MCM proteins with replication forks, previous immunofluorescence studies in mammalian cells have suggested that MCM 2-7 proteins are displaced after replication initiation from sites of DNA replication. Therefore, we used a robust statistical method to more precisely analyse immunofluorescence localization of MCM 2 proteins with respect to the DNA replication foci. We show that despite the predominantly different localization of MCM 2 and replication signals, there is still a small but significant fraction of MCM 2 proteins that co-localize with DNA replication foci during most of S phase. The fluorescence localization of the MCM 2 proteins and DNA replication may thus reflect an active function of MCM 2 pro"@en . . "57" . "A Fraction of MCM 2 Proteins Remain Associated with Replication Foci During a Major Part of S Phase" . . "[97FD4CE1056D]" . . . "I, P(LC535), S, Z(MSM0021620806)" . . . . "http://lge.lf1.cuni.cz/pdf/p2.pdf" . . "DNA replication; MCM proteins; immunofluorescence; replication foci; MCM paradox"@en . "0015-5500" . . "183802" . . . "RIV/00216208:11120/11:00002949!RIV12-MSM-11120___" . "000288623700002" . . "Ma\u0161ata, Martin" . . "5"^^ . "11120" . . "RIV/00216208:11120/11:00002949" . . . .