. . "[B1FF8EF62845]" . "Plasma EBV-DNA monitoring in Epstein-Barr virus-positive Hodgkin lymphoma patients" . . "000285153600002" . . "220504" . "Plasma EBV-DNA monitoring in Epstein-Barr virus-positive Hodgkin lymphoma patients"@en . . "Epstein-Barr virus (EBV) is associated with approximately one-third of Hodgkin lymphoma (HL) cases. EBV-DNA is often present in the plasma and whole blood of EBV-associated HL patients. However, the significance of EBV-DNA monitoring is debated. In a cohort of 165 adult HL patients, EBV-DNA viral load was prospectively monitored both in the plasma and whole blood. Diagnostic tissue samples of all patients were histologically reviewed; in 72% nodular sclerosis was detected, 24% presented with mixed cellularity (MC), and 5% had other type of HL. Tissues from 150 patients were also analyzed for the presence of latent EBV infection using in situ hybridization for EBV-encoded RNA (EBER) and immunohistochemistry for latent membrane protein (LMP1). Using these methods, 29 (19%) patients were classified as EBV positive. Using real-time quantitative PCR, 22 (76%) of EBV-positive HL patients had detectable EBV-DNA in the plasma and 19 (66%) patients in whole blood prior to therapy."@en . "Koz\u00E1k, Tom\u00E1\u0161" . . . . "I, P(NR9166), S" . . . . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Vernerov\u00E1, Zde\u0148ka" . "Markov\u00E1, Jana" . "Epstein-Barr virus (EBV) is associated with approximately one-third of Hodgkin lymphoma (HL) cases. EBV-DNA is often present in the plasma and whole blood of EBV-associated HL patients. However, the significance of EBV-DNA monitoring is debated. In a cohort of 165 adult HL patients, EBV-DNA viral load was prospectively monitored both in the plasma and whole blood. Diagnostic tissue samples of all patients were histologically reviewed; in 72% nodular sclerosis was detected, 24% presented with mixed cellularity (MC), and 5% had other type of HL. Tissues from 150 patients were also analyzed for the presence of latent EBV infection using in situ hybridization for EBV-encoded RNA (EBER) and immunohistochemistry for latent membrane protein (LMP1). Using these methods, 29 (19%) patients were classified as EBV positive. Using real-time quantitative PCR, 22 (76%) of EBV-positive HL patients had detectable EBV-DNA in the plasma and 19 (66%) patients in whole blood prior to therapy." . "Plasma EBV-DNA monitoring in Epstein-Barr virus-positive Hodgkin lymphoma patients"@en . . . "http://dx.doi.org/10.1111/j.1600-0463.2010.02685.x" . "Plasma EBV-DNA monitoring in Epstein-Barr virus-positive Hodgkin lymphoma patients" . . "Hodgkin lymphoma; Epstein-Barr virus; PCR; LMP1; EBER"@en . "8"^^ . . "RIV/00216208:11120/11:00002462!RIV12-MZ0-11120___" . . . "1" . "11120" . "\u0160pa\u010Dek, Martin" . . "RIV/00216208:11120/11:00002462" . "10.1111/j.1600-0463.2010.02685.x" . "7"^^ . . "4"^^ . "APMIS" . "119" . . . . "0903-4641" .