. . "Physiological Research" . "11120" . "CZ - \u010Cesk\u00E1 republika" . . "Genetic determinants of folate status in Central Bohemia" . "Jano\u0161\u00EDkov\u00E1, B." . . . "folates; folate hydrolase 1; reduced folate carrier; 5,10-methylenetetrahydrofolate reductase; polymorphism"@en . "Zv\u00E1rov\u00E1, J." . . . . "Pavl\u00EDkov\u00E1, M." . "RIV/00216208:11120/05:00001336!RIV09-MZ0-11120___" . "1"^^ . . "Genetick\u00E9 vymezen\u00ED stavu fol\u00E1t\u016F ve st\u0159edn\u00EDch \u010Cech\u00E1ch"@cs . "Although several genetic factors have been implicated as determinants of blood folate concentration in various populations, their effect on folate status in the Czech population has not yet been examined. We explored whether blood folate concentrations in healthy Czech population are associated with polymorphisms in 5,10-methylenetetrahydrofolate reductase (MTHFR), Folate hydrolase 1 (FOLH1), reduced folate carrier (RFC), and folate receptor (FOLR1) genes. In a cross-sectional study of 591 control subjects we determined genotypes by PCR-RFLP or ARMS-PCR methods, and plasma and erythrocyte folates by MEIA. The effect of different genotypes on folate status was examined by non-parametric tests and by regression analysis. The prevalence of the MTHFR 677C > T, MTHFR 1298A > C, FOLH1 1561C > T, RFC 80G > A and FOLR1 480G > C variant alleles was 0.34, 0.33, 0.05, 0.44 and 0.00, respectively. Only the MTHFR 677C > T variant was significantly associated with plasma folate concentrations (med" . "Ko\u017Eich, V." . . "Vesel\u00E1, K." . "9"^^ . "[D22B0F6E5616]" . "000230306700005" . "54" . "7"^^ . "RIV/00216208:11120/05:00001336" . . . . "3" . "Genetic determinants of folate status in Central Bohemia"@en . . "And\u011Bl, Michal" . "522541" . "Genetick\u00E9 vymezen\u00ED stavu fol\u00E1t\u016F ve st\u0159edn\u00EDch \u010Cech\u00E1ch"@cs . . "Genetic determinants of folate status in Central Bohemia"@en . "0862-8408" . . . . . "Hy\u00E1nek, J." . . "Although several genetic factors have been implicated as determinants of blood folate concentration in various populations, their effect on folate status in the Czech population has not yet been examined. We explored whether blood folate concentrations in healthy Czech population are associated with polymorphisms in 5,10-methylenetetrahydrofolate reductase (MTHFR), Folate hydrolase 1 (FOLH1), reduced folate carrier (RFC), and folate receptor (FOLR1) genes. In a cross-sectional study of 591 control subjects we determined genotypes by PCR-RFLP or ARMS-PCR methods, and plasma and erythrocyte folates by MEIA. The effect of different genotypes on folate status was examined by non-parametric tests and by regression analysis. The prevalence of the MTHFR 677C > T, MTHFR 1298A > C, FOLH1 1561C > T, RFC 80G > A and FOLR1 480G > C variant alleles was 0.34, 0.33, 0.05, 0.44 and 0.00, respectively. Only the MTHFR 677C > T variant was significantly associated with plasma folate concentrations (med"@en . . "Auto\u0159i popisuj\u00ED genetick\u00E9 vymezen\u00ED stavu fol\u00E1t\u016F ve st\u0159edn\u00EDch \u010Cech\u00E1ch"@cs . "P(NA6497), P(NM6548), Z(MSM 111100003)" . "Genetic determinants of folate status in Central Bohemia" .