"1059-1524" . . "2" . . . . "5"^^ . "Mel\u010D\u00E1k, Ivo" . "12" . . "Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles"@en . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . . "Ra\u0161ka, Ivan" . "P(GA302/99/0587), P(GA304/00/1481), P(VS96129), Z(MSM 111100003)" . "Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles"@en . . "Kopsk\u00FD, Vojt\u011Bch" . "RIV/00216208:11120/01:00003804" . . "RIV/00216208:11120/01:00003804!RIV12-MSM-11120___" . "Nuclear speckles (speckles) represent a distinct nuclear compartment within the interchromatin space and are enriched in splicing factors. They have been shown to serve neighboring active genes as a reservoir of these factors. In this study, we show that, in HeLa cells, the (pre)spliceosomal assembly on precursor mRNA (pre-mRNA) is associated with the speckles. For this purpose, we used microinjection of splicing competent and mutant adenovirus pre-mRNAs with differential splicing factor binding, which form different (pre)spliceosomal complexes and followed their sites of accumulation. Splicing competent pre-mRNAs are rapidly targeted into the speckles, but the targeting is temperature-dependent. The polypyrimidine tract sequence is required for targeting, but, in itself, is not sufficient. The downstream flanking sequences are particularly important for the targeting of the mutant pre-mRNAs into the speckles. In supportive experiments, the behavior of the speckles was followed after the microinjectio" . . . "[1DB13147DA05]" . "11120" . . . . "Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles" . "Nuclear speckles (speckles) represent a distinct nuclear compartment within the interchromatin space and are enriched in splicing factors. They have been shown to serve neighboring active genes as a reservoir of these factors. In this study, we show that, in HeLa cells, the (pre)spliceosomal assembly on precursor mRNA (pre-mRNA) is associated with the speckles. For this purpose, we used microinjection of splicing competent and mutant adenovirus pre-mRNAs with differential splicing factor binding, which form different (pre)spliceosomal complexes and followed their sites of accumulation. Splicing competent pre-mRNAs are rapidly targeted into the speckles, but the targeting is temperature-dependent. The polypyrimidine tract sequence is required for targeting, but, in itself, is not sufficient. The downstream flanking sequences are particularly important for the targeting of the mutant pre-mRNAs into the speckles. In supportive experiments, the behavior of the speckles was followed after the microinjectio"@en . . . . "692605" . . "Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles" . . . "Molecular Biology of the Cell" . . . "000170348000012" . "Pre-Messenger-Rna; Polymerase-Ii Transcription; Ribonucleoprotein-Particles; Splicing Factors; Living Cells; Nascent Transcripts; Functional U1; Sc-35 Domains; Sr Proteins; Localization"@en . . . . . . . "3"^^ . "14"^^ .