. . . . . "SK - Slovensk\u00E1 republika" . "676040" . "Comparison of two different methods for CD34+ selection and T cell depletion in peripheral blood stem cell grafts - Our experiences with CellPro, E rosetting and CliniMACS technique" . . . . "RIV/00216208:11120/01:00003672!RIV12-MSM-11120___" . "CD34+selection; T cell depletion; PBSC; immunorosettes; CellPro; CliniMACS"@en . "P(IZ3561), P(NE4554), Z(MSM 111200002)" . "Comparison of two different methods for CD34+ selection and T cell depletion in peripheral blood stem cell grafts - Our experiences with CellPro, E rosetting and CliniMACS technique"@en . "The aim of this study was to establish a suitable method for in vitro T cell depletion in peripheral blood ste in cell grafts for mismatched/haploidentical transplantation in children and adults with severe hematological disorders and for autologous transplantation in patients with autoimmune diseases refractory to conventional immunosuppressive treatment. Two different selection techniques have been used: CD34+ selection using immunoaffinity columns (CellPro Ceprate) followed by T cell depletion by E-rosetting or CD34+ selection using submicroscopic paramagnetic beads (CliniMACS device) with T cell depletion in a one step procedure. The mean purity and recovery of CD34+ cells and efficiency of T cell removal in the final product were compared" . "Polou\u010Dkov\u00E1, A." . "The aim of this study was to establish a suitable method for in vitro T cell depletion in peripheral blood ste in cell grafts for mismatched/haploidentical transplantation in children and adults with severe hematological disorders and for autologous transplantation in patients with autoimmune diseases refractory to conventional immunosuppressive treatment. Two different selection techniques have been used: CD34+ selection using immunoaffinity columns (CellPro Ceprate) followed by T cell depletion by E-rosetting or CD34+ selection using submicroscopic paramagnetic beads (CliniMACS device) with T cell depletion in a one step procedure. The mean purity and recovery of CD34+ cells and efficiency of T cell removal in the final product were compared"@en . "0028-2685" . "8"^^ . . . "RIV/00216208:11120/01:00003672" . . "11120" . "10"^^ . . "Comparison of two different methods for CD34+ selection and T cell depletion in peripheral blood stem cell grafts - Our experiences with CellPro, E rosetting and CliniMACS technique" . "5" . . "Neoplasma" . . . . . "48" . "Comparison of two different methods for CD34+ selection and T cell depletion in peripheral blood stem cell grafts - Our experiences with CellPro, E rosetting and CliniMACS technique"@en . . "[43D73CF071CA]" . "1"^^ . . "000172665400007" . . . "Koz\u00E1k, Tom\u00E1\u0161" .