"000319953100004" . . "81172" . "4"^^ . . . "Ho\u0159\u00EDnek, Ale\u0161" . "HO-1; NOS-2; Lipopolysaccharide; D-Galactosamine; Hepatotoxicity; Quercetin"@en . . "[023F66C5824F]" . . "The objective of this study was to evaluate potential hepatoprotective capabilities of quercetin in relation to its modulation of the HO-1 and NOS-2 activities in an experimental model of fulminant liver failure. Liver insult was induced by in vivo administration of D-galactosamine (D-GaIN, 400 mg/kg, i.p.) and lipopolysaccharide (LPS, 10 mu g/kg, i.p.). The effects of quercetin (50 mg/kg, i.p) on D-GaIN toxicity was evaluated by standard biochemical, RT-PCR and Western blot methods. Administration of D-GaIN/LPS combination resulted in significantly higher plasma levels of aminotransferases, as well as increased mRNA and protein expressions of both HO-1 and NOS-2 enzymes. Quercetin exhibited cytoprotective effects on the liver, as evidenced by decreased aminotransferase plasma levels. Additionally, quercetin treatment in D-GaIN/LPS treated rats significantly increased HO-1 mRNA and its protein expressions. On the contrary, quercetin did not exhibit any significant effects on the levels of nitrites, and NOS-2 mRNA and protein expressions in D-GaIN/LPS treated rats. Quercetin when given alone did not have any significant changes on liver enzymes nor HO-1 and NOS-2 mRNA and protein expressions. It can be concluded that the quercetin's induction of HO-1 and its byproducts, without concomitant NOS-2 activity reduction, is among mechanisms contributing to the hepatoprotective effect in D-GaIN/LPS hepatotoxicity." . "87" . "11110" . "Farghali, Hassan" . . . . "10.1016/j.fitote.2013.03.016" . . . "The objective of this study was to evaluate potential hepatoprotective capabilities of quercetin in relation to its modulation of the HO-1 and NOS-2 activities in an experimental model of fulminant liver failure. Liver insult was induced by in vivo administration of D-galactosamine (D-GaIN, 400 mg/kg, i.p.) and lipopolysaccharide (LPS, 10 mu g/kg, i.p.). The effects of quercetin (50 mg/kg, i.p) on D-GaIN toxicity was evaluated by standard biochemical, RT-PCR and Western blot methods. Administration of D-GaIN/LPS combination resulted in significantly higher plasma levels of aminotransferases, as well as increased mRNA and protein expressions of both HO-1 and NOS-2 enzymes. Quercetin exhibited cytoprotective effects on the liver, as evidenced by decreased aminotransferase plasma levels. Additionally, quercetin treatment in D-GaIN/LPS treated rats significantly increased HO-1 mRNA and its protein expressions. On the contrary, quercetin did not exhibit any significant effects on the levels of nitrites, and NOS-2 mRNA and protein expressions in D-GaIN/LPS treated rats. Quercetin when given alone did not have any significant changes on liver enzymes nor HO-1 and NOS-2 mRNA and protein expressions. It can be concluded that the quercetin's induction of HO-1 and its byproducts, without concomitant NOS-2 activity reduction, is among mechanisms contributing to the hepatoprotective effect in D-GaIN/LPS hepatotoxicity."@en . "The involvement of heme oxygenase 1 but not nitric oxide synthase 2 in a hepatoprotective action of quercetin in lipopolysaccharide-induced hepatotoxicity of D-galactosamine sensitized rats" . "I, S" . "Lekic, Nata\u0161a" . "The involvement of heme oxygenase 1 but not nitric oxide synthase 2 in a hepatoprotective action of quercetin in lipopolysaccharide-induced hepatotoxicity of D-galactosamine sensitized rats" . . . "The involvement of heme oxygenase 1 but not nitric oxide synthase 2 in a hepatoprotective action of quercetin in lipopolysaccharide-induced hepatotoxicity of D-galactosamine sensitized rats"@en . "Lekic, Nata\u0161a" . . "0367-326X" . "The involvement of heme oxygenase 1 but not nitric oxide synthase 2 in a hepatoprotective action of quercetin in lipopolysaccharide-induced hepatotoxicity of D-galactosamine sensitized rats"@en . "RIV/00216208:11110/13:10195513!RIV14-MSM-11110___" . . . "Kutinov\u00E1-Canov\u00E1, Nikolina" . "NL - Nizozemsko" . . "Fitoterapia" . . "JUN" . "7"^^ . "4"^^ . . . "http://dx.doi.org/10.1016/j.fitote.2013.03.016" . . "RIV/00216208:11110/13:10195513" . .