"4"^^ . . "Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples" . "Barto\u0161\u00EDk, Martin" . "4"^^ . "Vojt\u011B\u0161ek, Bo\u0159ivoj" . "Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples" . "Electrochemical methods hold promise for fast and inexpensive determination of cancer biomarkers, including microRNAs playing an important role in regulation of gene expression. We developed a simple assay utilizing (i) microRNA labeling with osmium(VI) complexes, (ii) hybridization of microRNA with magnetic bead-immobilized capture probe, and (iii) adsorptive transfer stripping (AdTS) technique in which working electrode is dipped into a microliter-sized drop for direct adsorption of microRNA. Increased sensitivity and decreased sample consumption was achieved, and nanogram quantities of microRNA present in 500-fold excess of total RNA were determined. Our results indicate only negligible interference during hybridization from other RNA molecules."@en . . "Hrstka, Roman" . "RIV/00209805:_____/14:#0000564!RIV15-GA0-00209805" . "RIV/00209805:_____/14:#0000564" . . "26" . "Barto\u0161\u00EDk, Martin" . . . "I, P(ED2.1.00/03.0101), P(GA13-00956S), P(GP14-24931P)" . "5"^^ . . . . "000345978300002" . "cancer; mercury electrode; microRNA; voltammetry"@en . . "[BCA9D96A8630]" . "Electrochemical methods hold promise for fast and inexpensive determination of cancer biomarkers, including microRNAs playing an important role in regulation of gene expression. We developed a simple assay utilizing (i) microRNA labeling with osmium(VI) complexes, (ii) hybridization of microRNA with magnetic bead-immobilized capture probe, and (iii) adsorptive transfer stripping (AdTS) technique in which working electrode is dipped into a microliter-sized drop for direct adsorption of microRNA. Increased sensitivity and decreased sample consumption was achieved, and nanogram quantities of microRNA present in 500-fold excess of total RNA were determined. Our results indicate only negligible interference during hybridization from other RNA molecules." . . "October 31" . "Electroanalysis" . "Pale\u010Dek, Emil" . . "10.1002/elan.201400449" . . . . . . . . "1040-0397" . "Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples"@en . "http://onlinelibrary.wiley.com/doi/10.1002/elan.201400449/abstract" . . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . "Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples"@en . "1713" . .