. "Vojt\u011B\u0161ek, Bo\u0159ivoj" . "Elektrochemick\u00E1 anal\u00FDza nukleov\u00FDch kyselin, b\u00EDlkovin a polysacharid\u016F v biomedic\u00EDn\u011B"@cs . "Pale\u010Dek, Emil" . "Barto\u0161\u00EDk, Martin" . "Elektrochemick\u00E1 anal\u00FDza nukleov\u00FDch kyselin, b\u00EDlkovin a polysacharid\u016F v biomedic\u00EDn\u011B" . "Barto\u0161\u00EDk, Martin" . "RIV/00209805:_____/14:#0000529" . "Electrochemical analysis of nucleic acids, proteins and polysaccharides represents an interesting, although not widely spread alternative to current methods based predominantly on optical detection because it offers a relatively inexpensive, fast and instrumentally simple detection of parallel samples on miniaturized chips, ideal for personalized medicine of the 21st century. Nucleic acid electrochemistry enables, for example, detection of specific DNA sequences (for determination of genes or presence of bacteria and viruses, etc.), DNA damage analysis and interaction with other molecules, DNA methylation or detection of microRNAs as potential cancer biomarkers. In the electrochemistry of proteins, great emphasis is put on construction of immunosensors for capturing specific proteins (antigens) using antibodies, suitable for diagnostics. From a biophysical point of view, intrinsic electrocatalytic signal of proteins sensitive to conformational changes could be useful in discrimination of mutant proteins (e. g. p53), native and aggregated forms (\u03B1-synuclein in Parkinson\u02BCs disease) or for studies of protein interactions with low molecular-weight ligands and DNA. Due to an increased interest of scientists in glycoproteins, new electrochemical papers emerged aiming at detection of oligosaccharides and polysaccharides (i.e. glycans, when part of the protein). These assays employ for instance electroactive labels specific for saccharides or lectin biosensors using lectins which strongly bind glycans. Electrochemical analysis thus appears as an interesting tool in current genomics, proteomics and glycomics, as well as for cancer dia gnostics."@en . . . . . . . "Elektrochemick\u00E1 anal\u00FDza nukleov\u00FDch kyselin, b\u00EDlkovin a polysacharid\u016F v biomedic\u00EDn\u011B" . "[08CB2817F366]" . . . . . "Klinick\u00E1 onkologie" . "3"^^ . . . "8"^^ . . "electrochemistry; electrodes; nucleic acid hybridization; DNA sensors; electrocatalysis; biological markers"@en . "3"^^ . "27" . . "http://www.linkos.cz/files/klinicka-onkologie/186/4481.pdf" . "supplementum 1" . "Elektrochemick\u00E1 anal\u00FDza nukleov\u00FDch kyselin, b\u00EDlkovin i polysacharid\u016F p\u0159edstavuje zaj\u00EDmavou, i kdy\u017E zat\u00EDm m\u00E9n\u011B pou\u017E\u00EDvanou alternativu ve srovn\u00E1n\u00ED se st\u00E1vaj\u00EDc\u00EDmi metodami zalo\u017Een\u00FDmi zejm\u00E9na na optick\u00E9 detekci. Nab\u00EDz\u00ED toti\u017E relativn\u011B levnou, rychlou a p\u0159\u00EDstrojov\u011B nen\u00E1ro\u010Dnou mo\u017Enost paraleln\u00ED detekce na miniaturizovan\u00FDch \u010Dipech, ide\u00E1ln\u00ED pro personalizovanou medic\u00EDnu 21. stolet\u00ED. Elektrochemie nukleov\u00FDch kyselin umo\u017E\u0148uje nap\u0159. detekci konkr\u00E9tn\u00EDch sekvenc\u00ED DNA (pro ur\u010Den\u00ED gen\u016F, stanoven\u00ED p\u0159\u00EDtomnosti bakteri\u00ED a vir\u016F, atd.), anal\u00FDzu po\u0161kozen\u00ED DNA a interakc\u00ED s jin\u00FDmi molekulami, DNA metylaci, nebo detekci mikroRNA jako n\u00E1dorov\u00FDch bio marker\u016F. V elektrochemii b\u00EDlkovin je v sou\u010Dasnosti kladen d\u016Fraz zejm\u00E9na na konstrukci tzv. imunosenzor\u016F schopn\u00FDch detekce konkr\u00E9tn\u00EDch protein\u016F (antigen\u016F) pomoc\u00ED jejich vazby na protil\u00E1tku, s potenci\u00E1lem pro diagnostiku. Z biofyzik\u00E1ln\u00EDho hlediska je zaj\u00EDmav\u00FD vlastn\u00ED elektrokatalytick\u00FD sign\u00E1l b\u00EDlkovin citliv\u00FD k jejich konforma\u010Dn\u00EDm zm\u011Bn\u00E1m, kter\u00FD by mohl nal\u00E9zt uplatn\u011Bn\u00ED p\u0159i rozli\u0161en\u00ED mutantn\u00EDch forem protein\u016F (nap\u0159. u p53) nebo p\u0159i jejich agregaci (\u03B1-synuklein v p\u0159\u00EDpad\u011B Parkinsonovy nemoci), pop\u0159\u00EDpad\u011B p\u0159i studiu interakc\u00ED s n\u00EDzkomolekul\u00E1rn\u00EDmi l\u00E1tkami \u010Di DNA. D\u00EDky zv\u00FD\u0161en\u00E9mu z\u00E1jmu o glykoproteiny se za\u010D\u00EDnaj\u00ED objevovat elektrochemick\u00E9 pr\u00E1ce zab\u00FDvaj\u00EDc\u00ED se detekc\u00ED oligosacharid\u016F a polysacharid\u016F (tzv. glykan\u016F, pokud jsou sou\u010D\u00E1st\u00ED b\u00EDlkovin), a to pomoc\u00ED elektroaktivn\u00EDch zna\u010Dek specifick\u00FDch pro sacharidy anebo konstrukc\u00ED lektinov\u00FDch biosenzor\u016F vyu\u017E\u00EDvaj\u00EDc\u00EDch lektin\u016F, kter\u00E9 se siln\u011B v\u00E1\u017E\u00ED pr\u00E1v\u011B na glykany. Elektrochemick\u00E1 anal\u00FDza se tak jev\u00ED jako zaj\u00EDmav\u00FD n\u00E1stroj v sou\u010Dasn\u00E9m v\u00FDzkumu na poli genomiky, proteomiky i glykomiky, v\u010Detn\u011B dia gnostiky n\u00E1dorov\u00FDch onemocn\u011Bn\u00ED."@cs . "CZ - \u010Cesk\u00E1 republika" . "Electrochemical analysis of nucleic acids, proteins and polysaccharides in biomedicine"@en . . "Electrochemical analysis of nucleic acids, proteins and polysaccharides in biomedicine"@en . . "I, P(ED2.1.00/03.0101), P(GP14-24931P)" . "0862-495X" . "Elektrochemick\u00E1 anal\u00FDza nukleov\u00FDch kyselin, b\u00EDlkovin i polysacharid\u016F p\u0159edstavuje zaj\u00EDmavou, i kdy\u017E zat\u00EDm m\u00E9n\u011B pou\u017E\u00EDvanou alternativu ve srovn\u00E1n\u00ED se st\u00E1vaj\u00EDc\u00EDmi metodami zalo\u017Een\u00FDmi zejm\u00E9na na optick\u00E9 detekci. Nab\u00EDz\u00ED toti\u017E relativn\u011B levnou, rychlou a p\u0159\u00EDstrojov\u011B nen\u00E1ro\u010Dnou mo\u017Enost paraleln\u00ED detekce na miniaturizovan\u00FDch \u010Dipech, ide\u00E1ln\u00ED pro personalizovanou medic\u00EDnu 21. stolet\u00ED. Elektrochemie nukleov\u00FDch kyselin umo\u017E\u0148uje nap\u0159. detekci konkr\u00E9tn\u00EDch sekvenc\u00ED DNA (pro ur\u010Den\u00ED gen\u016F, stanoven\u00ED p\u0159\u00EDtomnosti bakteri\u00ED a vir\u016F, atd.), anal\u00FDzu po\u0161kozen\u00ED DNA a interakc\u00ED s jin\u00FDmi molekulami, DNA metylaci, nebo detekci mikroRNA jako n\u00E1dorov\u00FDch bio marker\u016F. V elektrochemii b\u00EDlkovin je v sou\u010Dasnosti kladen d\u016Fraz zejm\u00E9na na konstrukci tzv. imunosenzor\u016F schopn\u00FDch detekce konkr\u00E9tn\u00EDch protein\u016F (antigen\u016F) pomoc\u00ED jejich vazby na protil\u00E1tku, s potenci\u00E1lem pro diagnostiku. Z biofyzik\u00E1ln\u00EDho hlediska je zaj\u00EDmav\u00FD vlastn\u00ED elektrokatalytick\u00FD sign\u00E1l b\u00EDlkovin citliv\u00FD k jejich konforma\u010Dn\u00EDm zm\u011Bn\u00E1m, kter\u00FD by mohl nal\u00E9zt uplatn\u011Bn\u00ED p\u0159i rozli\u0161en\u00ED mutantn\u00EDch forem protein\u016F (nap\u0159. u p53) nebo p\u0159i jejich agregaci (\u03B1-synuklein v p\u0159\u00EDpad\u011B Parkinsonovy nemoci), pop\u0159\u00EDpad\u011B p\u0159i studiu interakc\u00ED s n\u00EDzkomolekul\u00E1rn\u00EDmi l\u00E1tkami \u010Di DNA. D\u00EDky zv\u00FD\u0161en\u00E9mu z\u00E1jmu o glykoproteiny se za\u010D\u00EDnaj\u00ED objevovat elektrochemick\u00E9 pr\u00E1ce zab\u00FDvaj\u00EDc\u00ED se detekc\u00ED oligosacharid\u016F a polysacharid\u016F (tzv. glykan\u016F, pokud jsou sou\u010D\u00E1st\u00ED b\u00EDlkovin), a to pomoc\u00ED elektroaktivn\u00EDch zna\u010Dek specifick\u00FDch pro sacharidy anebo konstrukc\u00ED lektinov\u00FDch biosenzor\u016F vyu\u017E\u00EDvaj\u00EDc\u00EDch lektin\u016F, kter\u00E9 se siln\u011B v\u00E1\u017E\u00ED pr\u00E1v\u011B na glykany. Elektrochemick\u00E1 anal\u00FDza se tak jev\u00ED jako zaj\u00EDmav\u00FD n\u00E1stroj v sou\u010Dasn\u00E9m v\u00FDzkumu na poli genomiky, proteomiky i glykomiky, v\u010Detn\u011B dia gnostiky n\u00E1dorov\u00FDch onemocn\u011Bn\u00ED." . . "14241" . . . "RIV/00209805:_____/14:#0000529!RIV15-GA0-00209805" . "Elektrochemick\u00E1 anal\u00FDza nukleov\u00FDch kyselin, b\u00EDlkovin a polysacharid\u016F v biomedic\u00EDn\u011B"@cs . .