"CZ - \u010Cesk\u00E1 republika" . "Chemick\u00E9 listy" . . "208673" . "Kvantifikace proteinov\u00FDch biomarker\u016F pomoc\u00ED hmotnostn\u00ED spektrometrie pracuj\u00EDc\u00ED v re\u017Eimu monitorov\u00E1n\u00ED vybran\u00FDch reakc\u00ED" . . "0009-2770" . "Quantitation of protein biomarkers by mass spectrometry in selected reaction monitoring mode"@en . "RIV/00209805:_____/11:#0000153" . "http://www.chemicke-listy.cz/docs/full/2011_11_846-850.pdf" . "6"^^ . . . . "Kvantifikace proteinov\u00FDch biomarker\u016F pomoc\u00ED hmotnostn\u00ED spektrometrie pracuj\u00EDc\u00ED v re\u017Eimu monitorov\u00E1n\u00ED vybran\u00FDch reakc\u00ED"@cs . . "RIV/00209805:_____/11:#0000153!RIV12-MSM-00209805" . "Quantitation of protein biomarkers by mass spectrometry in selected reaction monitoring mode"@en . . "Monitorov\u00E1n\u00ED vybran\u00FDch reakc\u00ED (SRM) pat\u0159\u00ED do kvantitativn\u00EDch metod hmotnostn\u00ED spektrometrie, kter\u00E9 lze tak\u00E9 uplatnit v anal\u00FDze peptid\u016F. Hmotnostn\u00ED spektrometry ur\u010Den\u00E9 pro SRM anal\u00FDzu jsou spojeny s nanoHPLC, maj\u00ED iontov\u00FD zdroj nanoelektrosprej a trojit\u00FD kvadrup\u00F3lov\u00FD analyz\u00E1tor. V prinicpu, prvn\u00ED kvadrupol funguje jako hmotnostn\u00ED filtr, kter\u00FD propust\u00ED pouze vybran\u00FD peptidov\u00FD iont, kter\u00FD je fragmentov\u00E1n v druh\u00E9m kvadrup\u00F3lu quadupole p\u0159es kolizn\u00ED celu. T\u0159et\u00ED kvadrup\u00F3l p\u0159en\u00E1\u0161\u00ED vybran\u00E9 peptidoc\u00E9 fragmenty sm\u011Brem k detektoru. Tento p\u0159\u00EDstup umo\u017E\u0148uje c\u00EDlenou kvantifikaci des\u00EDtek protein\u016F v r\u00E1mci jedin\u00E9 anal\u00FDzy a to s velmi dobrou citlivost\u00ED a selektivnost\u00ED. SRM se tak st\u00E1v\u00E1 kl\u00ED\u010Dovou metodou proteomiky. Hlavn\u00EDm odv\u011Btv\u00EDm jej\u00EDho pou\u017Eit\u00ED spo\u010D\u00EDv\u00E1 v rychl\u00E9m stanoven\u00ED protein\u016F v r\u00E1mci rozs\u00E1hl\u00FDch soubor\u016F vzork\u016F." . "3"^^ . . . . . "Monitorov\u00E1n\u00ED vybran\u00FDch reakc\u00ED (SRM) pat\u0159\u00ED do kvantitativn\u00EDch metod hmotnostn\u00ED spektrometrie, kter\u00E9 lze tak\u00E9 uplatnit v anal\u00FDze peptid\u016F. Hmotnostn\u00ED spektrometry ur\u010Den\u00E9 pro SRM anal\u00FDzu jsou spojeny s nanoHPLC, maj\u00ED iontov\u00FD zdroj nanoelektrosprej a trojit\u00FD kvadrup\u00F3lov\u00FD analyz\u00E1tor. V prinicpu, prvn\u00ED kvadrupol funguje jako hmotnostn\u00ED filtr, kter\u00FD propust\u00ED pouze vybran\u00FD peptidov\u00FD iont, kter\u00FD je fragmentov\u00E1n v druh\u00E9m kvadrup\u00F3lu quadupole p\u0159es kolizn\u00ED celu. T\u0159et\u00ED kvadrup\u00F3l p\u0159en\u00E1\u0161\u00ED vybran\u00E9 peptidoc\u00E9 fragmenty sm\u011Brem k detektoru. Tento p\u0159\u00EDstup umo\u017E\u0148uje c\u00EDlenou kvantifikaci des\u00EDtek protein\u016F v r\u00E1mci jedin\u00E9 anal\u00FDzy a to s velmi dobrou citlivost\u00ED a selektivnost\u00ED. SRM se tak st\u00E1v\u00E1 kl\u00ED\u010Dovou metodou proteomiky. Hlavn\u00EDm odv\u011Btv\u00EDm jej\u00EDho pou\u017Eit\u00ED spo\u010D\u00EDv\u00E1 v rychl\u00E9m stanoven\u00ED protein\u016F v r\u00E1mci rozs\u00E1hl\u00FDch soubor\u016F vzork\u016F."@cs . "Kvantifikace proteinov\u00FDch biomarker\u016F pomoc\u00ED hmotnostn\u00ED spektrometrie pracuj\u00EDc\u00ED v re\u017Eimu monitorov\u00E1n\u00ED vybran\u00FDch reakc\u00ED" . "P(ED2.1.00/03.0101), P(GAP304/10/0868), Z(MSM0021622413), Z(MZ0MOU2005)" . . "000297567400004" . . "protein quantitation; mass spektrometry; proteomics"@en . . "Struh\u00E1rov\u00E1, Iva" . "11" . . "Kvantifikace proteinov\u00FDch biomarker\u016F pomoc\u00ED hmotnostn\u00ED spektrometrie pracuj\u00EDc\u00ED v re\u017Eimu monitorov\u00E1n\u00ED vybran\u00FDch reakc\u00ED"@cs . . "105" . "5"^^ . . "[72B09224F4D1]" . . . . "Selected reaction monitoring (SRM) is a quantitative MS-based method which recently expanded to analysis of proteins by determination of peptides. The instrumentation consists of a nanoflow liquid chromatograph coupled with nanoelectrospray ionization and a triple-quadrupole mass spectrometer. In principle, the first quadrupole works as a mass filter, transmitting only a selected peptide, which is fragmented in a second quadupole via collision-induced dissociation. The third quadrupole transmits the selected peptide fragments, which are then directed to the detector. This approach enables targeted quantification of tens of proteins in a single analysis with very good sensitivity and selectivity. The SRM becomes a key method in a new targeted-proteomics toolbox. The most important field of its applications lies in the life sciences research where SRM enables fast, accurate and cost-effective determination of proteins in large sets of samples."@en . "Vojt\u011B\u0161ek, Bo\u0159ivoj" . . "Bouchal, Pavel" . .