"9"^^ . "We found that phosphorylation of p53 by CK2 on Ser392 induces its DNA binding aktivity of p53 to a much lower extent than phosphorylation by cdk2/cyclin A or PKC. In addition, we found that phosphorylation by CK2 strongly inhibits PKC-induced activation of p53 DNA binding, while the activation of p53 by cdk2/cyclin A is not affected by CK2. The presence of CK2-mediated phosphorylation promotes PKC binding to its docking site within the p53 oligomerization domain, but decreases phosphorylation of p53 by PKC, suggesting that competition between CK2 and PKC does not rely on the inhibition of PKC?p53 complex formation. These results indicate the crucial role of p53 C-terminal phosphorylation in the regulation of its DNA-binding activity, but also suggest that antagonistic relationships exist between different stress signalling pathways." . "Activation of DNA binding ability of latent p53 protein by protein kinase C is abolished by casein kinase II" . "939; 947" . "Activation of DNA binding ability of latent p53 protein by protein kinase C is abolished by casein kinase II"@en . . "8"^^ . . . . "We found that phosphorylation of p53 by CK2 on Ser392 induces its DNA binding aktivity of p53 to a much lower extent than phosphorylation by cdk2/cyclin A or PKC. In addition, we found that phosphorylation by CK2 strongly inhibits PKC-induced activation of p53 DNA binding, while the activation of p53 by cdk2/cyclin A is not affected by CK2. The presence of CK2-mediated phosphorylation promotes PKC binding to its docking site within the p53 oligomerization domain, but decreases phosphorylation of p53 by PKC, suggesting that competition between CK2 and PKC does not rely on the inhibition of PKC?p53 complex formation. These results indicate the crucial role of p53 C-terminal phosphorylation in the regulation of its DNA-binding activity, but also suggest that antagonistic relationships exist between different stress signalling pathways."@en . "RIV/00209805:_____/04:00013927!RIV/2005/GA0/L26005/N" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "pt. 3" . "RIV/00209805:_____/04:00013927" . . . "P(GA301/02/0831)" . "0264-6021" . . . . . "[F9F4EDC19AAF]" . "Activation of DNA binding ability of latent p53 protein by protein kinase C is abolished by casein kinase II" . "Zjistili jsme \u017Ee fosforylace p53 CK2 na Ser392 indukuje DNA vazebnou aktivitu p53 mnohem m\u00E9n\u011B ne\u017E fosforylace cdk2/cyclin A nebo PKC. Nav\u00EDc jsme zjistili, \u017Ee fosforylace CK2 siln\u011B inhibuje PKC indukovanou aktivaci vazby p53 na DNA, zat\u00EDmco aktivace p53 cdk2/cyclin A nen\u00ED ovlivn\u011Bna. Fosforylace CK2 zvy\u0161uje vazbu PKC do jej\u00EDho vazebn\u00E9ho m\u00EDsta v p53 oligomeriza\u010Dn\u00ED dom\u00E9n\u011B, ale p\u0159itom sni\u017Euje fosforylaci p53 PKC. Tento poznatek potvrzuje, \u017Ee kompetici mezi CK2 a PKC nen\u00ED z\u00E1visl\u00E1 na inhibici tvorby komplexu p53-PKC. Tyto v\u00FDsledky potvrzuj\u00ED d\u016Fle\u017Eitost C-koncov\u00E9 fosforylace p\u0159i regulaci DNA vazebn\u00E9 aktivity proteinu p53 a sou\u010Dasn\u011B potvrzuj\u00ED antagonistick\u00FD vztah existuj\u00EDc\u00ED mezi rozd\u00EDln\u00FDmi stresov\u00FDmi drahami."@cs . "Aktivace DNA vazebne schopnosti latentni formy p53 protin kinasou C je inhibovana kasein kinasou II"@cs . . . . "Activation of DNA binding ability of latent p53 protein by protein kinase C is abolished by casein kinase II"@en . "Vojt\u011B\u0161ek, Bo\u0159ivoj" . . . . . "Aktivace DNA vazebne schopnosti latentni formy p53 protin kinasou C je inhibovana kasein kinasou II"@cs . "378" . "The Biochemical journal" . "553448" . "activation;cdk2/cyclin A;DNA binding;electrophoretic mobility shift assay (EMSA);p53;phosphorylation"@en . "2"^^ .