. "Kest\u0159\u00E1nek, Jan" . . "RIV/00179906:_____/12:10128441!RIV13-MZ0-00179906" . "polyadenylated primer; PCR; sequencing; polymorphism; DNA"@en . "Buchta, Vladim\u00EDr" . . . "106" . "5"^^ . "\u0160pa\u010Dek, Ji\u0159\u00ED" . "Single-Strand Sequencing of PCR Products of the MBL2 Gene with a Polyadenylated Sequencing Primer"@en . . . . . "[A688DBE0FE82]" . . "RIV/00179906:_____/12:10128441" . . "Petera, Ji\u0159\u00ED" . "8" . "Jednosm\u011Brn\u00E9 sekvenov\u00E1n\u00ED PCR produkt\u016F MBL2 genu pomoc\u00ED polyadenylovan\u00E9ho sekvena\u010Dn\u00EDho primeru" . "6"^^ . . "http://www.chemicke-listy.cz/docs/full/2012_08_783-787.pdf" . "Single-Strand Sequencing of PCR Products of the MBL2 Gene with a Polyadenylated Sequencing Primer"@en . . "Chemick\u00E9 listy" . . . "Jednosm\u011Brn\u00E9 sekvenov\u00E1n\u00ED PCR produkt\u016F MBL2 genu pomoc\u00ED polyadenylovan\u00E9ho sekvena\u010Dn\u00EDho primeru"@cs . "In a group of 24 patients with recurrent vulvovaginal candidiasis and 24 healthy subjects we analysed polymorphic sites in the 5' untranslated region (5' UTR) and codons 52, 54, and 57 of the MBL2 gene. Using PCR we amplified 317 bp MBL2 products covering all the polymorphisms. Purified amplicons were used in cycle sequencing reactions containing conventional or polyadenylated forward primers. We revealed that the polyadenylated primer used in the cycle sequencing extends the readable range of MBL2 amplicons without necessity of doublestrand sequencing, subcloning or reamplification with other pair of primers. Our preliminary data showed that the distribution of MBL2 alleles, haplotypes, and genotypes in the patients and healthy subjects was very similar and the differences were not statistically significant."@en . "Drast\u00EDkov\u00E1, Monika" . "000314233700011" . "143672" . "Jednosm\u011Brn\u00E9 sekvenov\u00E1n\u00ED PCR produkt\u016F MBL2 genu pomoc\u00ED polyadenylovan\u00E9ho sekvena\u010Dn\u00EDho primeru"@cs . "6"^^ . "0009-2770" . "CZ - \u010Cesk\u00E1 republika" . . . "Ve skupin\u011B 24 pacientek s rekurentn\u00ED vulvovagin\u00E1ln\u00ED kandid\u00F3zou a 24 zdrav\u00FDch \u017Een jsme analyzovali polymorfn\u00ED m\u00EDsta v 5' nep\u0159ekl\u00E1dan\u00E9m konci a kodonech 52, 54 a 57 genu MBL2. Pomoc\u00ED PCR jsme amplifikovali produkty o d\u00E9lce 317 bp, kter\u00E9 obsahovaly v\u0161echny zm\u00EDn\u011Bn\u00E9 polymorfn\u00ED m\u00EDsta. Purifikovan\u00E9 amplikony vyly pou\u017Eity pro sekvena\u010Dn\u00ED reakce obsahuj\u00EDc\u00ED konven\u010Dn\u00ED a polyadenylovan\u00E9 p\u0159\u00EDm\u00E9 (forward) primery. Prok\u00E1zali jsme, \u017Ee polyadenylovan\u00FD primer pou\u017Eit\u00FD p\u0159i sekvena\u010Dn\u00ED reakci prodlu\u017Euje \u010Ditelnost MBL2 amplikon\u016F bez nutnosti oboustrann\u00E9ho sekvenov\u00E1n\u00ED, subklonov\u00E1n\u00ED nebo reamplifikace s jin\u00FDm p\u00E1rem primer\u016F. Na\u0161e p\u0159edb\u011B\u017En\u00E1 data uk\u00E1zala, \u017Ee distribuce alel, haplotyp\u016F a genotyp\u016F genu MBL2 u osob vulvovagin\u00E1ln\u00ED kandid\u00F3zou a zdrav\u00FDch osob se vz\u00E1jemn\u011B statisticky v\u00FDznamn\u011B neli\u0161\u00ED." . . . . . "I, P(LC531), P(NT11334)" . . "Ve skupin\u011B 24 pacientek s rekurentn\u00ED vulvovagin\u00E1ln\u00ED kandid\u00F3zou a 24 zdrav\u00FDch \u017Een jsme analyzovali polymorfn\u00ED m\u00EDsta v 5' nep\u0159ekl\u00E1dan\u00E9m konci a kodonech 52, 54 a 57 genu MBL2. Pomoc\u00ED PCR jsme amplifikovali produkty o d\u00E9lce 317 bp, kter\u00E9 obsahovaly v\u0161echny zm\u00EDn\u011Bn\u00E9 polymorfn\u00ED m\u00EDsta. Purifikovan\u00E9 amplikony vyly pou\u017Eity pro sekvena\u010Dn\u00ED reakce obsahuj\u00EDc\u00ED konven\u010Dn\u00ED a polyadenylovan\u00E9 p\u0159\u00EDm\u00E9 (forward) primery. Prok\u00E1zali jsme, \u017Ee polyadenylovan\u00FD primer pou\u017Eit\u00FD p\u0159i sekvena\u010Dn\u00ED reakci prodlu\u017Euje \u010Ditelnost MBL2 amplikon\u016F bez nutnosti oboustrann\u00E9ho sekvenov\u00E1n\u00ED, subklonov\u00E1n\u00ED nebo reamplifikace s jin\u00FDm p\u00E1rem primer\u016F. Na\u0161e p\u0159edb\u011B\u017En\u00E1 data uk\u00E1zala, \u017Ee distribuce alel, haplotyp\u016F a genotyp\u016F genu MBL2 u osob vulvovagin\u00E1ln\u00ED kandid\u00F3zou a zdrav\u00FDch osob se vz\u00E1jemn\u011B statisticky v\u00FDznamn\u011B neli\u0161\u00ED."@cs . "Ber\u00E1nek, Martin" . . "Jednosm\u011Brn\u00E9 sekvenov\u00E1n\u00ED PCR produkt\u016F MBL2 genu pomoc\u00ED polyadenylovan\u00E9ho sekvena\u010Dn\u00EDho primeru" . . . .