"RIV/00179906:_____/12:10124568!RIV13-MZ0-00179906" . "Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia" . . . . . "[BC1732549AC5]" . "10.1179/1607845412Y.0000000015" . "Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia" . . "Zeta-associated protein of 70 kDa (ZAP-70) is a tyrosine kinase that plays a role in signal transduction from the T-cell receptor. ZAP-70 is expressed in normal T-cells and NK-cells. Increased expression of ZAP-70 has been identified in chronic lymphocytic leukemia (CLL). CLL patients with increased ZAP-70 expression have significantly worse prognosis in terms of both progression-free survival and overall survival. There are several methods to quantify ZAP-70: polymerase chain reaction (PCR), immunoblotting, immunohistochemistry, and flow cytometry. Use of flow cytometry for ZAP-70 detection seems to be advantageous as this technique enables us to assess the presence of ZAP-70 separately on CLL clone, T-cells, and NK-cells. On the other hand, detection of ZAP-70 by flow cytometry is substantially influenced by many variables. The principal drawback of flow cytometry is the absence of consensus regarding selection of optimal anti-ZAP-70 antibody, fluorochrome conjugate, the most reliable staining technique, and optimal positivity threshold. This article summarizes pitfalls of flow cytometric analysis of ZAP-70 in CLL."@en . "Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia"@en . . "3"^^ . "000308350000004" . . . . "Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia"@en . . "158766" . . "3"^^ . "1024-5332" . . "RIV/00179906:_____/12:10124568" . . "http://dx.doi.org/10.1179/1607845412Y.0000000015" . . "I, P(NT13412)" . "17" . "Standardization; Prognosis; Flow cytometry; CLL; ZAP-70"@en . . . "Zeta-associated protein of 70 kDa (ZAP-70) is a tyrosine kinase that plays a role in signal transduction from the T-cell receptor. ZAP-70 is expressed in normal T-cells and NK-cells. Increased expression of ZAP-70 has been identified in chronic lymphocytic leukemia (CLL). CLL patients with increased ZAP-70 expression have significantly worse prognosis in terms of both progression-free survival and overall survival. There are several methods to quantify ZAP-70: polymerase chain reaction (PCR), immunoblotting, immunohistochemistry, and flow cytometry. Use of flow cytometry for ZAP-70 detection seems to be advantageous as this technique enables us to assess the presence of ZAP-70 separately on CLL clone, T-cells, and NK-cells. On the other hand, detection of ZAP-70 by flow cytometry is substantially influenced by many variables. The principal drawback of flow cytometry is the absence of consensus regarding selection of optimal anti-ZAP-70 antibody, fluorochrome conjugate, the most reliable staining technique, and optimal positivity threshold. This article summarizes pitfalls of flow cytometric analysis of ZAP-70 in CLL." . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Hematology" . "5" . . "Krejsek, Jan" . "Smolej, Luk\u00E1\u0161" . . "7"^^ . . . "Vroblov\u00E1, Vladim\u00EDra" .