"77105" . "HDL: funkce, dysfunkce a laboratorn\u00ED metody stanoven\u00ED" . "HDL: function, dysfunction and laboratory methods of determination"@en . "HDL: funkce, dysfunkce a laboratorn\u00ED metody stanoven\u00ED" . "RIV/00098892:_____/13:#0000500" . "HDL: funkce, dysfunkce a laboratorn\u00ED metody stanoven\u00ED"@cs . "RIV/00098892:_____/13:#0000500!RIV14-MZ0-00098892" . "HDL: funkce, dysfunkce a laboratorn\u00ED metody stanoven\u00ED"@cs . . . . "dysfunctional HDL, reverse cholesterol transport, inflammation, paraoxonase 1"@en . "Objective: on the basis of recent studies to make an overview concerning the crucial biological functions of high density lipoproteins (HDL), with emphasis on the role in reverse cholesterol transport and their antiinflammatory traits. The aim was to describe probable mechanisms of dysfunctional proinflammatory HDL formation with introduction some of associated parameters. To present laboratory methods for determination of quantitative, structural and functional qualities of HDL, including advanced mass spectrometry techniques. Conclusion: in the case of systemic inflammation and/or oxidative stress, the formation of dysfunctional HDL accumulating oxidants takes place, including apo AI structural modifications. This process can lead to the inhibition of reverse cholesterol transport and proinflammatory HDL generation, among others. Loss of beneficial qualities results from modification of both lipid and protein components of HDL. In some cases, an isolated quantitative measurement of HDL cholesterol may not fully correlate with cardiovascular risk and it is eligible to determine some of structural and/or funkctional markers of HDL."@en . "C\u00EDl: na z\u00E1klad\u011B soudob\u00FDch studi\u00ED podat p\u0159ehled o hlavn\u00EDch biologick\u00FDch funkc\u00EDch lipoprotein\u016F o vysok\u00E9 hustot\u011B (HDL), zejm\u00E9na ve vztahu k reverzn\u00EDmu transportu cholesterolu a k jejich protiz\u00E1n\u011Btliv\u00E9mu p\u016Fsoben\u00ED. Popsat pravd\u011Bpodobn\u00E9 mechanismy vzniku dysfunk\u010Dn\u00EDch proz\u00E1n\u011Btliv\u00FDch HDL a uv\u00E9st n\u011Bkter\u00E9 parametry asociovan\u00E9 s t\u00EDmto procesem. Podat p\u0159ehled o laboratorn\u00EDch metod\u00E1ch stanoven\u00ED kvantitativn\u00EDch, strukturn\u00EDch a funk\u010Dn\u00EDch vlastnost\u00ED HDL, v\u010Detn\u011B modern\u00EDch metod zalo\u017Een\u00FDch na hmotov\u00E9 spektrometrii. Z\u00E1v\u011Br: v p\u0159\u00EDpad\u011B syst\u00E9mov\u00E9ho z\u00E1n\u011Btu a/nebo oxida\u010Dn\u00EDho stresu doch\u00E1z\u00ED ke vzniku dysfunk\u010Dn\u00EDch HDL akumuluj\u00EDc\u00EDch oxidanty. Prob\u00EDh\u00E1 strukturn\u00ED modifikace apolipoproteinu AI, co\u017E vede mimo jin\u00E9 k inhibici reverzn\u00EDho transportu cholesterolu a tvorb\u011B proz\u00E1n\u011Btliv\u00FDch HDL. Na ztr\u00E1t\u011B p\u0159\u00EDzniv\u00FDch vlastnost\u00ED HDL se pod\u00EDl\u00ED modifikace proteinov\u00E9 i lipidov\u00E9 slo\u017Eky HDL. Izolovan\u00E9 m\u011B\u0159en\u00ED HDL cholesterolu nemus\u00ED u n\u011Bkter\u00FDch syndrom\u016F bezv\u00FDhradn\u011B korelovat s m\u00EDrou kardiovaskul\u00E1rn\u00EDho rizika a v n\u011Bkter\u00FDch situac\u00EDch je vhodn\u00E9 stanovit markery mapuj\u00EDc\u00ED strukturu a funkci HDL."@cs . . . "HDL: function, dysfunction and laboratory methods of determination"@en . "3" . "CZ - \u010Cesk\u00E1 republika" . . "I" . "7"^^ . "Klinick\u00E1 biochemie a metabolismus" . . . . . . . . "1"^^ . . "C\u00EDl: na z\u00E1klad\u011B soudob\u00FDch studi\u00ED podat p\u0159ehled o hlavn\u00EDch biologick\u00FDch funkc\u00EDch lipoprotein\u016F o vysok\u00E9 hustot\u011B (HDL), zejm\u00E9na ve vztahu k reverzn\u00EDmu transportu cholesterolu a k jejich protiz\u00E1n\u011Btliv\u00E9mu p\u016Fsoben\u00ED. Popsat pravd\u011Bpodobn\u00E9 mechanismy vzniku dysfunk\u010Dn\u00EDch proz\u00E1n\u011Btliv\u00FDch HDL a uv\u00E9st n\u011Bkter\u00E9 parametry asociovan\u00E9 s t\u00EDmto procesem. Podat p\u0159ehled o laboratorn\u00EDch metod\u00E1ch stanoven\u00ED kvantitativn\u00EDch, strukturn\u00EDch a funk\u010Dn\u00EDch vlastnost\u00ED HDL, v\u010Detn\u011B modern\u00EDch metod zalo\u017Een\u00FDch na hmotov\u00E9 spektrometrii. Z\u00E1v\u011Br: v p\u0159\u00EDpad\u011B syst\u00E9mov\u00E9ho z\u00E1n\u011Btu a/nebo oxida\u010Dn\u00EDho stresu doch\u00E1z\u00ED ke vzniku dysfunk\u010Dn\u00EDch HDL akumuluj\u00EDc\u00EDch oxidanty. Prob\u00EDh\u00E1 strukturn\u00ED modifikace apolipoproteinu AI, co\u017E vede mimo jin\u00E9 k inhibici reverzn\u00EDho transportu cholesterolu a tvorb\u011B proz\u00E1n\u011Btliv\u00FDch HDL. Na ztr\u00E1t\u011B p\u0159\u00EDzniv\u00FDch vlastnost\u00ED HDL se pod\u00EDl\u00ED modifikace proteinov\u00E9 i lipidov\u00E9 slo\u017Eky HDL. Izolovan\u00E9 m\u011B\u0159en\u00ED HDL cholesterolu nemus\u00ED u n\u011Bkter\u00FDch syndrom\u016F bezv\u00FDhradn\u011B korelovat s m\u00EDrou kardiovaskul\u00E1rn\u00EDho rizika a v n\u011Bkter\u00FDch situac\u00EDch je vhodn\u00E9 stanovit markery mapuj\u00EDc\u00ED strukturu a funkci HDL." . "1210-7921" . . "4"^^ . "21" . . "[FFFE88B51A63]" . "Novotn\u00FD, Dalibor" .