"Vliv matrixov\u00FDch metaloprotein\u00E1z na hojen\u00ED ran po operaci vrozen\u00E9ho roz\u0161t\u011Bpu rtu" . "Role of matrix metalloproteinases in wound healing after congenital cleft lip surgery"@en . . "9"^^ . . "Otoupalova, E." . . "Matejova, R." . . "1" . "RIV/00064203:_____/12:8329!RIV13-MZ0-00064203" . . "Role of matrix metalloproteinases in wound healing after congenital cleft lip surgery"@en . "Dost\u00E1lov\u00E1, Ta\u0165jana" . "RIV/00064203:_____/12:8329" . "Vliv matrixov\u00FDch metaloprotein\u00E1z na hojen\u00ED ran po operaci vrozen\u00E9ho roz\u0161t\u011Bpu rtu" . . "Borsky, J." . "http://www.prolekare.cz/cesko-slovenska-pediatrie-archiv-cisel?id=3654" . . "I, P(NS10012)" . "Cleft lip; Matrix metalloproteinases; Wound healing"@en . . . "177923" . . . . . . "Steklacova, A." . "C\u00EDlem bylo analyzovat slo\u017Eky extracelul\u00E1rn\u00ED matrix zodpov\u011Bdn\u00E9 za hojen\u00ED, p\u0159edev\u0161\u00EDm stanovit koncentrace matrixov\u00FDch metaloprotein\u00E1z (MMP-1, MMP-2 a MMP-3) v tk\u00E1ni odstran\u011Bn\u00E9 p\u0159i operaci. Porovnat hojen\u00ED ran po operaci roz\u0161t\u011Bpu rtu do sedmi dn\u016F po narozen\u00ED a hojen\u00ED ran po operaci podle klasick\u00E9ho protokolu ve t\u0159ech m\u011Bs\u00EDc\u00EDch v\u011Bku. Materi\u00E1l a metody: Vzorky tk\u00E1n\u011B byly odebr\u00E1ny od 30 d\u011Bt\u00ED (16 chlapc\u016F a 14 d\u00EDvek), 17 d\u011Bt\u00ED bylo operov\u00E1no b\u011Bhem prvn\u00EDho t\u00FDdne po narozen\u00ED a 13 d\u011Bt\u00ED bylo operov\u00E1no ve 2\u20134 m\u011Bs\u00EDc\u00EDch v\u011Bku. Stanoven\u00ED MMP bylo prov\u00E1d\u011Bno v tk\u00E1\u0148ov\u00FDch zbytc\u00EDch z\u00EDskan\u00FDch chirurgickou exciz\u00ED okraj\u016F roz\u0161t\u011Bpu rtu, kter\u00E9 jsou p\u0159i v\u00FDkonu odstra\u0148ov\u00E1ny jako nadbyte\u010Dn\u00E9. Po odb\u011Bru byla tk\u00E1\u0148 skladov\u00E1na zamrazen\u00EDm na -70 \u02DAC. Extrakce prob\u011Bhla v kakodyl\u00E1tov\u00E9m pufru po dobu 24 hodin p\u0159i teplot\u011B 2\u20138 \u02DAC. Ke stanoven\u00ED koncentrace celkov\u00E9 b\u00EDlkoviny v tk\u00E1ni byla zvolena Lowryho metoda. Koncentrace MMP-1, MMP-2, MMP-3 byly stanovov\u00E1ny metodou ELISA, pomoc\u00ED kit\u016F Amersham Biotrak Activity Assay (GE Healthcare UK). V\u00FDsledky: MMP-1: u d\u011Bt\u00ED operovan\u00FDch b\u011Bhem prvn\u00EDho t\u00FDdne po narozen\u00ED byly nam\u011B\u0159eny koncentrace 0,017 +/- 0,023 \u00B5g/g b\u00EDlkoviny (pr\u016Fm\u011Br +/- SD), u d\u011Bt\u00ED operovan\u00FDch ve 2\u20134 m\u011Bs\u00EDc\u00EDch v\u011Bku 0,028 +/- 0,026 \u00B5g/g b\u00EDlkoviny. MMP-3: u d\u011Bt\u00ED operovan\u00FDch b\u011Bhem prvn\u00EDho t\u00FDdne po narozen\u00ED byly nam\u011B\u0159eny koncentrace 0,200 +/- 0,142 \u00B5g/g b\u00EDlkoviny, u d\u011Bt\u00ED operovan\u00FDch ve 2\u20134 m\u011Bs\u00EDc\u00EDch v\u011Bku 0,155 +/- 0,093 \u00B5g/g b\u00EDlkoviny. Koncentrace MMP-2 byly ve v\u0161ech m\u011B\u0159en\u00FDch vzorc\u00EDch I po z\u0159ed\u011Bn\u00ED vy\u0161\u0161\u00ED ne\u017E horn\u00ED mez koncentra\u010Dn\u00EDho rozmez\u00ED kit\u016F 12 \u00B5g/l. Z\u00E1v\u011Br: Nezjistili jsme statisticky v\u00FDznamn\u00E9 rozd\u00EDly v koncentrac\u00EDch celkov\u00E9 b\u00EDlkoviny, \u010Di koncentrac\u00EDch MMP-1, MMP-2 a MMP-3 mezi skupinou d\u011Bt\u00ED operovan\u00FDch b\u011Bhem prvn\u00EDho t\u00FDdne po narozen\u00ED a skupinou d\u011Bt\u00ED operovan\u00FDch ve 2\u20134 m\u011Bs\u00EDc\u00EDch v\u011Bku."@cs . "Objective: The purpose of this study was to compare the aspects of wound healing after cleft lip surgery within one week of age and wound healing after surgery within 2-4 months of age. We examined extracellular matrix proteins involved in wound healing, especially concentrations of matrix metalloproteinases in tissue removed during surgery. Material and methods: 30 tissue samples (16 boys and 14 girls) were removed during surgery within one week of age 17 or within 2-4 months of age 13. Proteins were extracted in cacodylic buffer for 24 hours in temperature 2-8 \u00B0C. Total protein concentrations were examined using modification of Lowry method. Samples were examined using ELISA kit Amersham Biotrak Activity Assay (GE Healthcare UK) for detection of MMP-1, MMP-2, and MMP-3 concentrations. Results: MMP-1: early surgery 0.017 +/- 0.023 \u03BCg/g of protein (mean +/- SD), 2-4 months surgery 0.028 +/- 0.026 \u03BCg/g. MMP-3: early surgery 0.200 +/- 0.142 \u03BCg/g, 2-4 months surgery 0.155 +/- 0.093 \u03BCg/g. Concentrations of MMP-2 were after dilution above working range at 12 \u03BCg/l in all tested samples. Conclusion: No significant differences in concentrations of protein and MMPs, MMP-1 and MMP-3 according to time of surgery were found."@en . "\u010Cesko-slovensk\u00E1 pediatrie" . . "Pr\u016F\u0161a, Richard" . . "0069-2328" . . "Kota\u0161ka, Karel" . "C\u00EDlem bylo analyzovat slo\u017Eky extracelul\u00E1rn\u00ED matrix zodpov\u011Bdn\u00E9 za hojen\u00ED, p\u0159edev\u0161\u00EDm stanovit koncentrace matrixov\u00FDch metaloprotein\u00E1z (MMP-1, MMP-2 a MMP-3) v tk\u00E1ni odstran\u011Bn\u00E9 p\u0159i operaci. Porovnat hojen\u00ED ran po operaci roz\u0161t\u011Bpu rtu do sedmi dn\u016F po narozen\u00ED a hojen\u00ED ran po operaci podle klasick\u00E9ho protokolu ve t\u0159ech m\u011Bs\u00EDc\u00EDch v\u011Bku. Materi\u00E1l a metody: Vzorky tk\u00E1n\u011B byly odebr\u00E1ny od 30 d\u011Bt\u00ED (16 chlapc\u016F a 14 d\u00EDvek), 17 d\u011Bt\u00ED bylo operov\u00E1no b\u011Bhem prvn\u00EDho t\u00FDdne po narozen\u00ED a 13 d\u011Bt\u00ED bylo operov\u00E1no ve 2\u20134 m\u011Bs\u00EDc\u00EDch v\u011Bku. Stanoven\u00ED MMP bylo prov\u00E1d\u011Bno v tk\u00E1\u0148ov\u00FDch zbytc\u00EDch z\u00EDskan\u00FDch chirurgickou exciz\u00ED okraj\u016F roz\u0161t\u011Bpu rtu, kter\u00E9 jsou p\u0159i v\u00FDkonu odstra\u0148ov\u00E1ny jako nadbyte\u010Dn\u00E9. Po odb\u011Bru byla tk\u00E1\u0148 skladov\u00E1na zamrazen\u00EDm na -70 \u02DAC. Extrakce prob\u011Bhla v kakodyl\u00E1tov\u00E9m pufru po dobu 24 hodin p\u0159i teplot\u011B 2\u20138 \u02DAC. Ke stanoven\u00ED koncentrace celkov\u00E9 b\u00EDlkoviny v tk\u00E1ni byla zvolena Lowryho metoda. Koncentrace MMP-1, MMP-2, MMP-3 byly stanovov\u00E1ny metodou ELISA, pomoc\u00ED kit\u016F Amersham Biotrak Activity Assay (GE Healthcare UK). V\u00FDsledky: MMP-1: u d\u011Bt\u00ED operovan\u00FDch b\u011Bhem prvn\u00EDho t\u00FDdne po narozen\u00ED byly nam\u011B\u0159eny koncentrace 0,017 +/- 0,023 \u00B5g/g b\u00EDlkoviny (pr\u016Fm\u011Br +/- SD), u d\u011Bt\u00ED operovan\u00FDch ve 2\u20134 m\u011Bs\u00EDc\u00EDch v\u011Bku 0,028 +/- 0,026 \u00B5g/g b\u00EDlkoviny. MMP-3: u d\u011Bt\u00ED operovan\u00FDch b\u011Bhem prvn\u00EDho t\u00FDdne po narozen\u00ED byly nam\u011B\u0159eny koncentrace 0,200 +/- 0,142 \u00B5g/g b\u00EDlkoviny, u d\u011Bt\u00ED operovan\u00FDch ve 2\u20134 m\u011Bs\u00EDc\u00EDch v\u011Bku 0,155 +/- 0,093 \u00B5g/g b\u00EDlkoviny. Koncentrace MMP-2 byly ve v\u0161ech m\u011B\u0159en\u00FDch vzorc\u00EDch I po z\u0159ed\u011Bn\u00ED vy\u0161\u0161\u00ED ne\u017E horn\u00ED mez koncentra\u010Dn\u00EDho rozmez\u00ED kit\u016F 12 \u00B5g/l. Z\u00E1v\u011Br: Nezjistili jsme statisticky v\u00FDznamn\u00E9 rozd\u00EDly v koncentrac\u00EDch celkov\u00E9 b\u00EDlkoviny, \u010Di koncentrac\u00EDch MMP-1, MMP-2 a MMP-3 mezi skupinou d\u011Bt\u00ED operovan\u00FDch b\u011Bhem prvn\u00EDho t\u00FDdne po narozen\u00ED a skupinou d\u011Bt\u00ED operovan\u00FDch ve 2\u20134 m\u011Bs\u00EDc\u00EDch v\u011Bku." . . "67" . "Vliv matrixov\u00FDch metaloprotein\u00E1z na hojen\u00ED ran po operaci vrozen\u00E9ho roz\u0161t\u011Bpu rtu"@cs . . . "4"^^ . "Vliv matrixov\u00FDch metaloprotein\u00E1z na hojen\u00ED ran po operaci vrozen\u00E9ho roz\u0161t\u011Bpu rtu"@cs . "Pechov\u00E1, Marta" . . "5"^^ . "CZ - \u010Cesk\u00E1 republika" . "Bl\u00E1ha, Karel" . "[B0FE2623F6FF]" .