"Almeida, J." . . . "te Marvelde, JG" . "9" . . "000308342900004" . . "RIV/00064203:_____/12:8232" . . "P(NT12425), Z(MSM0021620813)" . . "Vidriales, MB" . . "25"^^ . "Orfao, A." . . "Perez, JJ" . "Asnafi, V." . "Lhermitte, L." . "EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols" . . "Szczepanski, T." . . "Flores-Montero, J." . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "The EU-supported EuroFlow Consortium aimed at innovation and standardization of immunophenotyping for diagnosis and classification of hematological malignancies by introducing 8-color flow cytometry with fully standardized laboratory procedures and antibody panels in order to achieve maximally comparable results among different laboratories. This required the selection of optimal combinations of compatible fluorochromes and the design and evaluation of adequate standard operating procedures (SOPs) for instrument setup, fluorescence compensation and sample preparation. Additionally, we developed software tools for the evaluation of individual antibody reagents and antibody panels. Each section describes what has been evaluated experimentally versus adopted based on existing data and experience. Multicentric evaluation demonstrated high levels of reproducibility based on strict implementation of the EuroFlow SOPs and antibody panels. Overall, the 6 years of extensive collaborative experiments and the analysis of hundreds of cell samples of patients and healthy controls in the EuroFlow centers have provided for the first time laboratory protocols and software tools for fully standardized 8-color flow cytometric immunophenotyping of normal and malignant leukocytes in bone marrow and blood; this has yielded highly comparable data sets, which can be integrated in a single database."@en . . . . "0887-6924" . "[56D55CFBCC4D]" . "Kalina, Tom\u00E1\u0161" . . . "flow cytometry; standardization; compensation; software; fluorochromes; immunophenotyping; minimal residual disease; chronic lymphoproliferative disorders; international consensus recommendations; acute lymphoblastic-leukemia; hematolymphoid neoplasia; hematologic neoplasia; medical indications; quality-control; compensation; reagents"@en . "Bottcher, S." . "Hru\u0161\u00E1k, Ond\u0159ej" . "21"^^ . "RIV/00064203:_____/12:8232!RIV13-MZ0-00064203" . . "http://dx.doi.org/10.1038/leu.2012.122" . "EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols"@en . "Leukemia" . "EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols"@en . . "Cullen, M." . . . . "134938" . "van Dongen, JJM" . . "Mendonca, A." . "26" . . "EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols" . . . . "The EU-supported EuroFlow Consortium aimed at innovation and standardization of immunophenotyping for diagnosis and classification of hematological malignancies by introducing 8-color flow cytometry with fully standardized laboratory procedures and antibody panels in order to achieve maximally comparable results among different laboratories. This required the selection of optimal combinations of compatible fluorochromes and the design and evaluation of adequate standard operating procedures (SOPs) for instrument setup, fluorescence compensation and sample preparation. Additionally, we developed software tools for the evaluation of individual antibody reagents and antibody panels. Each section describes what has been evaluated experimentally versus adopted based on existing data and experience. Multicentric evaluation demonstrated high levels of reproducibility based on strict implementation of the EuroFlow SOPs and antibody panels. Overall, the 6 years of extensive collaborative experiments and the analysis of hundreds of cell samples of patients and healthy controls in the EuroFlow centers have provided for the first time laboratory protocols and software tools for fully standardized 8-color flow cytometric immunophenotyping of normal and malignant leukocytes in bone marrow and blood; this has yielded highly comparable data sets, which can be integrated in a single database." . . "Martin-Ayuso, M." . "Mejst\u0159\u00EDkov\u00E1, Ester" . . "Ritgen, M." . . . "Sedek, L." . "3"^^ . . "de Tute, R." . "van der Velden, VHJ" .