"Zeptomole Electrochemical Detection of Metallothioneins"@en . "5" . "Wang, J." . "Zeptomole Electrochemical Detection of Metallothioneins" . . . "RIV/00064203:_____/10:6272!RIV11-MZ0-00064203" . "I, P(IAA401990701)" . . "plasma-mass spectrometry; cancer; chronopotentiometry; speciation; proteomics; proteins; level"@en . "Kizek, R." . "299929" . . . . . "Zeptomole Electrochemical Detection of Metallothioneins"@en . . "Eckschlager, Tom\u00E1\u0161" . "Petrlova, J." . "000279522500009" . . . . "Zeptomole Electrochemical Detection of Metallothioneins" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "6"^^ . . . "Adam, V." . "8"^^ . . "We used adsorptive transfer stripping technique coupled with CPSA for detection of cysteine, glutathione oxidized and reduced, phytochelatin, bovine serum albumin, and metallothionein (MT). We estimated detection limits down to tens of fg per ml. This method was applied to detect metallothioneins in serum from patients with breast cancer and in neuroblastoma cells resistant and sensitive to cisplatin. It was found that MT level in serum was almost twice higher as the level in healthy individuals. This paper brings results on the application of ultra-sensitive electroanalytical method for MT detection. It is worth mentioning that detection of other biologically important molecules is possible by this method. Coupling of this method with isolation by antibody-modified paramagnetic particles may be implemented to lab-on-chip instrument."@en . . "Trnkov\u00E1, L." . . "1932-6203" . . . "PLoS One" . "1"^^ . "We used adsorptive transfer stripping technique coupled with CPSA for detection of cysteine, glutathione oxidized and reduced, phytochelatin, bovine serum albumin, and metallothionein (MT). We estimated detection limits down to tens of fg per ml. This method was applied to detect metallothioneins in serum from patients with breast cancer and in neuroblastoma cells resistant and sensitive to cisplatin. It was found that MT level in serum was almost twice higher as the level in healthy individuals. This paper brings results on the application of ultra-sensitive electroanalytical method for MT detection. It is worth mentioning that detection of other biologically important molecules is possible by this method. Coupling of this method with isolation by antibody-modified paramagnetic particles may be implemented to lab-on-chip instrument." . "[ECC53BD87802]" . "RIV/00064203:_____/10:6272" . "7" . .