"8"^^ . "V" . "1" . . "Cx43; cardiomyocytes; beta-adrenoceptor; isoprenaline; MAPK; congestive heart failure; hypertrophic cardiomyopathy; human cardiac biopsy"@en . . . "The signal transduction cascade regulating the expression of the gap junction protein connexin43 by beta-adrenoceptors"@en . "341126" . "British Journal of Pharmacology" . . "1"^^ . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . . . . . "Janou\u0161ek, Jan" . "158" . "Blanke, K." . "BACKGROUND AND PURPOSE: In mammalian heart, connexin43 (Cx43) represents the predominant connexin in the working myocardium. As the beta-adrenoceptor is involved in many cardiac diseases, we wanted to clarify the pathway by which beta-adrenoceptor stimulation may control Cx43 expression. EXPERIMENTAL APPROACH: Cultured neonatal rat cardiomyocytes were stimulated with isoprenaline. Cx43 expression as well as activation of p38 mitogen-activated protein kinase (MAPK), p42/44 MAPK, JUN NH(2)-terminal kinase (JNK) and nuclear translocation of the transcription factors activator protein 1 (AP1) and CRE-binding protein (CREB) were investigated. Additionally, we assessed Cx43 expression and distribution in left ventricular biopsies from patients without any significant heart disease, and from patients with either congestive heart failure [dilated cardiomyopathy (DCM)] or hypertrophic cardiomyopathy (HCM). KEY RESULTS: Isoprenaline exposure caused about twofold up-regulation of Cx43 protein with a pEC(50) of 7"@en . "Salameh, Aida" . "000269315700020" . "The signal transduction cascade regulating the expression of the gap junction protein connexin43 by beta-adrenoceptors" . . "0007-1188" . "BACKGROUND AND PURPOSE: In mammalian heart, connexin43 (Cx43) represents the predominant connexin in the working myocardium. As the beta-adrenoceptor is involved in many cardiac diseases, we wanted to clarify the pathway by which beta-adrenoceptor stimulation may control Cx43 expression. EXPERIMENTAL APPROACH: Cultured neonatal rat cardiomyocytes were stimulated with isoprenaline. Cx43 expression as well as activation of p38 mitogen-activated protein kinase (MAPK), p42/44 MAPK, JUN NH(2)-terminal kinase (JNK) and nuclear translocation of the transcription factors activator protein 1 (AP1) and CRE-binding protein (CREB) were investigated. Additionally, we assessed Cx43 expression and distribution in left ventricular biopsies from patients without any significant heart disease, and from patients with either congestive heart failure [dilated cardiomyopathy (DCM)] or hypertrophic cardiomyopathy (HCM). KEY RESULTS: Isoprenaline exposure caused about twofold up-regulation of Cx43 protein with a pEC(50) of 7" . . "11"^^ . . "The signal transduction cascade regulating the expression of the gap junction protein connexin43 by beta-adrenoceptors"@en . . "Karl, S." . "Krautblatter, S." . . . "RIV/00064203:_____/09:5985!RIV10-MZ0-00064203" . . . "[F1CC08EB5BDE]" . . . "RIV/00064203:_____/09:5985" . "The signal transduction cascade regulating the expression of the gap junction protein connexin43 by beta-adrenoceptors" . "Dhein, S." . "Gomez, DR" . "Pfeiffer, D." .