"Bilirubin accumulation and Cyp mRNA expression in selected brain regions of jaundiced Gunn rat pups" . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Kon\u00ED\u010Dkov\u00E1, Renata" . . "INTRODUCTION: Few data exist on regional brain bilirubin content in the neonatal period when acute bilirubin-induced neurologic damage (BIND) may occur, and no information is available on regional brain expression of cytochrome P450 monooxygenases (Cyps) that oxidize bilirubin. METHODS: Bilirubin content was analyzed by high-performance liquid chromatography and Cyp1a1, 1a2, and 2a3 mRNA expression was analyzed by quantitative PCR (qPCR) in cortex (Cx), cerebellum (CII), superior colliculi (SC), and inferior colliculi (IC) of 17-d-old hyperbilirubinemic (jj) Gunn rat pups before and after administration of sulphadimethoxine to acutely displace bilirubin from plasma albumin. RESULTS:There was no difference in bilirubin content among brain regions in untreated rats. After intraperitoneal sulphadimethoxine, bilirubin content peaked at fourfold in Cx and SC at 1h; but at 11- to 13-fold in CII and IC at 24h; returning to control levels at 72h. The Cyp mRNA peaked at 30-70 times control at 1 h in Cx and SC, but at 3-9 times control at 24 h in CII and IC. DISCUSSION: The close relationship in distinct brain regions between the extent of bilirubin accumulation and induction of mRNA of Cyps suggests Cyps may have a role in protecting selected brain areas from bilirubin neurotoxicity." . "8"^^ . . "Bilirubin accumulation and Cyp mRNA expression in selected brain regions of jaundiced Gunn rat pups"@en . "Robert, Maria C." . "Pediatric Research" . "INTRODUCTION: Few data exist on regional brain bilirubin content in the neonatal period when acute bilirubin-induced neurologic damage (BIND) may occur, and no information is available on regional brain expression of cytochrome P450 monooxygenases (Cyps) that oxidize bilirubin. METHODS: Bilirubin content was analyzed by high-performance liquid chromatography and Cyp1a1, 1a2, and 2a3 mRNA expression was analyzed by quantitative PCR (qPCR) in cortex (Cx), cerebellum (CII), superior colliculi (SC), and inferior colliculi (IC) of 17-d-old hyperbilirubinemic (jj) Gunn rat pups before and after administration of sulphadimethoxine to acutely displace bilirubin from plasma albumin. RESULTS:There was no difference in bilirubin content among brain regions in untreated rats. After intraperitoneal sulphadimethoxine, bilirubin content peaked at fourfold in Cx and SC at 1h; but at 11- to 13-fold in CII and IC at 24h; returning to control levels at 72h. The Cyp mRNA peaked at 30-70 times control at 1 h in Cx and SC, but at 3-9 times control at 24 h in CII and IC. DISCUSSION: The close relationship in distinct brain regions between the extent of bilirubin accumulation and induction of mRNA of Cyps suggests Cyps may have a role in protecting selected brain areas from bilirubin neurotoxicity."@en . "Bilirubin accumulation and Cyp mRNA expression in selected brain regions of jaundiced Gunn rat pups"@en . . . "71" . "000304296200005" . . "V\u00EDtek, Libor" . . "Gazzin, Silvia" . . . "Bilirubin accumulation and Cyp mRNA expression in selected brain regions of jaundiced Gunn rat pups" . . "http://dx.doi.org/10.1038/pr.2012.23" . "124942" . "1"^^ . "6" . "Giraudi, Pablo J." . . "0031-3998" . . "Zdr\u00E1halov\u00E1, Lucie" . . "Zabetta, Carlos Coda" . "Bilirubin; unconjugated bilirubin; CYP mRNA; cytochrome-p450; hyperbilirubinemia; Crigler-Najjar syndrome; BIND; albumin plasma; cytotoxicity"@en . . . . . . "RIV/00064165:_____/12:13165" . "Tiribelli, Claudio" . "RIV/00064165:_____/12:13165!RIV13-MZ0-00064165" . "Berengeno, Andrea L." . "11"^^ . . . "Zelenka, Jaroslav" . "I, P(2B06155), P(GAP206/11/0836)" . . "Raseni, Alan" . . "[3B9B5B55A015]" . . .