. "Matulov\u00E1, Marta" . . "Plos One" . "In this study we were interested in identification of new markers of chicken response to Salmonella Enteritidis infection. To reach this aim, gene expression in the spleens of naive chickens and those intravenously infected with S. Enteritidis with or without previous oral vaccination was determined by 454 pyrosequencing of splenic mRNA/cDNA. Forty genes with increased expression at the level of transcription were identified. The most inducible genes encoded avidin (AVD), extracellular fatty acid binding protein (EXFABP), immune responsive gene 1 (IRG1), chemokine ah221 (AH221), trappin-6-like protein (TRAP6) and serum amyloid A (SAA). Using cDNA from sorted splenic B-lymphocytes, macrophages, CD4, CD8 and gamma delta T-lymphocytes, we found that the above mentioned genes were preferentially expressed in macrophages. AVD, EXFABP, IRG1, AH221, TRAP6 and SAA were induced also in the cecum of chickens orally infected with S. Enteritidis on day 1 of life or day 42 of life. Unusual results were obtained for the immunoglobulin encoding transcripts. Prior to the infection, transcripts coding for the constant parts of IgM, IgY, IgA and Ig light chain were detected in B-lymphocytes. However, after the infection, immunoglobulin encoding transcripts were expressed also by T-lymphocytes and macrophages. Expression of AVD, EXFABP, IRG1, AH221, TRAP6, SAA and all immunoglobulin genes can be therefore used for the characterization of the course of S. Enteritidis infection in chickens."@en . . "Volf, Ji\u0159\u00ED" . . . . . . "Vlasat\u00EDkov\u00E1, Lenka" . . "Characterization of Chicken Spleen Transcriptome after Infection with Salmonella enterica Serovar Enteritidis"@en . . . "RIV/00027162:_____/12:#0000933" . . . "P(ED0006/01/01), P(GAP502/12/0303), Z(MZE0002716202)" . "000310050200059" . . . . "\u0160i\u0161\u00E1k, Franti\u0161ek" . . . "R\u00E1jov\u00E1, Jana" . . . . . "10.1371/journal.pone.0048101" . "RIV/00027162:_____/12:#0000933!RIV13-MZE-00027162" . "7"^^ . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . . "7"^^ . "Characterization of Chicken Spleen Transcriptome after Infection with Salmonella enterica Serovar Enteritidis" . "[1F48D32A261A]" . . . "126807" . "10" . "1932-6203" . . . "Characterization of Chicken Spleen Transcriptome after Infection with Salmonella enterica Serovar Enteritidis" . . "SUPEROXIDE-DISMUTASE PLAYS; IMMUNE-RESPONSE; CELL-PROLIFERATION; IN-VIVO; B-CELLS; EXPRESSION; TYPHIMURIUM; LIPOPOLYSACCHARIDE; MACROPHAGES; INHIBITION"@en . "7" . "In this study we were interested in identification of new markers of chicken response to Salmonella Enteritidis infection. To reach this aim, gene expression in the spleens of naive chickens and those intravenously infected with S. Enteritidis with or without previous oral vaccination was determined by 454 pyrosequencing of splenic mRNA/cDNA. Forty genes with increased expression at the level of transcription were identified. The most inducible genes encoded avidin (AVD), extracellular fatty acid binding protein (EXFABP), immune responsive gene 1 (IRG1), chemokine ah221 (AH221), trappin-6-like protein (TRAP6) and serum amyloid A (SAA). Using cDNA from sorted splenic B-lymphocytes, macrophages, CD4, CD8 and gamma delta T-lymphocytes, we found that the above mentioned genes were preferentially expressed in macrophages. AVD, EXFABP, IRG1, AH221, TRAP6 and SAA were induced also in the cecum of chickens orally infected with S. Enteritidis on day 1 of life or day 42 of life. Unusual results were obtained for the immunoglobulin encoding transcripts. Prior to the infection, transcripts coding for the constant parts of IgM, IgY, IgA and Ig light chain were detected in B-lymphocytes. However, after the infection, immunoglobulin encoding transcripts were expressed also by T-lymphocytes and macrophages. Expression of AVD, EXFABP, IRG1, AH221, TRAP6, SAA and all immunoglobulin genes can be therefore used for the characterization of the course of S. Enteritidis infection in chickens." . "Rychl\u00EDk, Ivan" . "Characterization of Chicken Spleen Transcriptome after Infection with Salmonella enterica Serovar Enteritidis"@en . "9"^^ . "\u0160t\u011Bp\u00E1nov\u00E1, Hana" . . .