. "\u0160t\u011Bp\u00E1nov\u00E1, Hana" . "Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis"@en . "Kaevska, Marija" . . . "G\u00F6pfert, Eduard" . "176263" . . "RIV/00027162:_____/12:#0000920" . "Faldyna, Martin" . . "24" . "[ADF8E20D7745]" . . "2" . "Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis" . "Slan\u00E1, Iva" . . "10.1177/1040638711435141" . . . . . . "Pavl\u00EDk, Ivo" . . "7"^^ . . . "000307114500021" . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis"@en . "Gamma interferon; infection; tuberculination"@en . "Kaevska, Marija" . "7"^^ . . "3"^^ . "P(1B53009), P(ED0006/01/01), Z(MZE0002716202)" . "RIV/00027162:_____/12:#0000920!RIV13-MZE-00027162" . . . "Kudl\u00E1\u010Dkov\u00E1, Hana" . "Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis" . . "Journal of Veterinary Diagnostic Investigation" . "In the current study, the results of an intradermal tuberculin test and a gamma interferon (INF-?) release assay were compared based on the detection of interferon-gamma production after in vitro stimulation with Mycobacterium avium subsp. avium-specific antigen for the discrimination of pigs naturally infected with M. avium subsp. hominissuis. Fifty-five clinically healthy pigs were used in the study. Three of these were proven by culture and real-time quantitative polymerase chain reaction methods to be infected with M. avium subsp. hominissuis (2 animals) and Mycobacterium xenopi (1 animal). No animals were positive by the tuberculin test. Both M. avium subsp. hominissuis-positive pigs were evaluated as positive by the INF-? release assay. Bacteriologically negative and M. xenopi-positive pigs were unresponsive in the INF-? release assay, indicating the specificity of the method. The results suggest that the INF-? release assay has a higher sensitivity than the tuberculin test and that the assay can be used for diagnosis of M. avium infections in live, naturally infected pigs." . . "1040-6387" . "In the current study, the results of an intradermal tuberculin test and a gamma interferon (INF-?) release assay were compared based on the detection of interferon-gamma production after in vitro stimulation with Mycobacterium avium subsp. avium-specific antigen for the discrimination of pigs naturally infected with M. avium subsp. hominissuis. Fifty-five clinically healthy pigs were used in the study. Three of these were proven by culture and real-time quantitative polymerase chain reaction methods to be infected with M. avium subsp. hominissuis (2 animals) and Mycobacterium xenopi (1 animal). No animals were positive by the tuberculin test. Both M. avium subsp. hominissuis-positive pigs were evaluated as positive by the INF-? release assay. Bacteriologically negative and M. xenopi-positive pigs were unresponsive in the INF-? release assay, indicating the specificity of the method. The results suggest that the INF-? release assay has a higher sensitivity than the tuberculin test and that the assay can be used for diagnosis of M. avium infections in live, naturally infected pigs."@en . .