"RIV/00027162:_____/12:#0000917" . . "Men\u0161\u00EDkov\u00E1, Mark\u00E9ta" . . "000303099200003" . "4"^^ . . . . "Chlebov\u00E1, Katar\u00EDna" . "ACTINOBACILLUS-PLEUROPNEUMONIAE INFECTION; SALMONELLA-ENTERICA; LYMPHOID-TISSUE; IMMUNE-SYSTEM; CELLS; PIGLETS; EXPRESSION; IL-17; PIGS; DISTINCT"@en . . . "6"^^ . . "10.1016/j.cyto.2012.01.004" . . "126170" . "Cytokine" . . "In the veterinary field, only limited information is available about interleukin-17A (IL-17), despite the fact that this cytokine plays an important role during pro-inflammatory immune responses and induces the production of chemotactic factors for neutrophils. The aim of this study was to characterize porcine IL-17-producing cells. We tested the cross-reactivity of five anti-human IL-17 monoclonal antibodies because such antibodies against porcine IL-17 are currently unavailable. Whole blood cells (WBCs) were stimulated with phorbol-myristate-acetate (PMA) and ionomycin and subsequently analyzed by flow cytometry. The antibody clone SCPL1362 was found to cross-react with porcine IL-17, whereas the other four antibodies tested did not recognize this cytokine. Using this antibody, we characterized porcine WBC-secreting IL-17 after PMA and ionomycin stimulation. All IL-17-producing WBCs were positive for the T lymphocyte marker CD3. Myeloid cells (CD172 alpha(+)) and B lymphocytes (CD79 alpha(+)) were IL-17 negative. The major subset of IL-17 positive T lymphocytes was the CD4(+) lymphocytes (about 60% of all IL-17 positive WBCs). The remaining IL-17 positive WBCs were gamma delta TCR+ lymphocytes. CD8 positive and CD8 negative cells were found within both CD4(+) and gamma delta TCR+ cells producing the cytokine. Moreover, IL-17 positive cells were mostly CD45RA negative, therefore activated cells or memory cells. Flow cytometry data were confirmed using sorted cells. Both sorted CD4(+) and gamma delta TCR+ cells produced IL-17 at mRNA level after PMA and ionomycin stimulation while double negative CD4(-)gamma delta TCR- cells were negative for IL-17. We can conclude that only two subpopulations of porcine WBCs are sources of IL-17 after non-specific stimulation: CD3(+)CD4(+) and CD3(+)gamma delta TCR+. (C) 2012." . . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . . "RIV/00027162:_____/12:#0000917!RIV13-MZE-00027162" . "In the veterinary field, only limited information is available about interleukin-17A (IL-17), despite the fact that this cytokine plays an important role during pro-inflammatory immune responses and induces the production of chemotactic factors for neutrophils. The aim of this study was to characterize porcine IL-17-producing cells. We tested the cross-reactivity of five anti-human IL-17 monoclonal antibodies because such antibodies against porcine IL-17 are currently unavailable. Whole blood cells (WBCs) were stimulated with phorbol-myristate-acetate (PMA) and ionomycin and subsequently analyzed by flow cytometry. The antibody clone SCPL1362 was found to cross-react with porcine IL-17, whereas the other four antibodies tested did not recognize this cytokine. Using this antibody, we characterized porcine WBC-secreting IL-17 after PMA and ionomycin stimulation. All IL-17-producing WBCs were positive for the T lymphocyte marker CD3. Myeloid cells (CD172 alpha(+)) and B lymphocytes (CD79 alpha(+)) were IL-17 negative. The major subset of IL-17 positive T lymphocytes was the CD4(+) lymphocytes (about 60% of all IL-17 positive WBCs). The remaining IL-17 positive WBCs were gamma delta TCR+ lymphocytes. CD8 positive and CD8 negative cells were found within both CD4(+) and gamma delta TCR+ cells producing the cytokine. Moreover, IL-17 positive cells were mostly CD45RA negative, therefore activated cells or memory cells. Flow cytometry data were confirmed using sorted cells. Both sorted CD4(+) and gamma delta TCR+ cells produced IL-17 at mRNA level after PMA and ionomycin stimulation while double negative CD4(-)gamma delta TCR- cells were negative for IL-17. We can conclude that only two subpopulations of porcine WBCs are sources of IL-17 after non-specific stimulation: CD3(+)CD4(+) and CD3(+)gamma delta TCR+. (C) 2012."@en . . "CD4(+) and gamma delta TCR+ T lymphocytes are sources of interleukin-17 in swine" . . "2" . . "\u0160t\u011Bp\u00E1nov\u00E1, Hana" . . . "1043-4666" . . "P(ED0006/01/01), P(GA524/08/1606), Z(MZE0002716202)" . "58" . "CD4(+) and gamma delta TCR+ T lymphocytes are sources of interleukin-17 in swine"@en . . . "CD4(+) and gamma delta TCR+ T lymphocytes are sources of interleukin-17 in swine" . . . . "Faldyna, Martin" . . "4"^^ . . "[7467CA73D848]" . . . "CD4(+) and gamma delta TCR+ T lymphocytes are sources of interleukin-17 in swine"@en .