. "Faldyna, Martin" . . . "Adenosine is a well described anti-inflammatory modulator of immune responses. The aim of the present study was to describe the role of common adenosine agonist 5'-N-ethylcarboxamidoadenosine (NECA) in cytokine production by main porcine T cell subpopulations. TNF-alpha, IFN-gamma, IL-2 and IL-10 were detected by multicolor flow cytometry together with cell surface markers CD3, CD4 and CD8. It was found that NECA inhibits (in a dose-dependent manner) production of pro-inflammatory TNF-alpha and Th1-associated cytokines IFN-gamma, IL-2 in all concanavalin A-stimulated T cell subpopulations. Moreover, production of IL-10 was potentiated in all T cell subpopulations tested. These corresponded well with the fact that all T cell subsets expressed mRNA for adenosine receptor (AR) subtypes to comparable extents. Contrary to concanavalin A-stimulated cells, NECA had a moderate effect on PMA-stimulated T cells, suggesting that AR in pigs acts via signaling pathways not associated with protein-kinase C. Non-selective antagonist CGS15943 as well as allosteric modulator SCH202676 failed to reverse the effect of NECA in pigs. In conclusion, NECA has an anti-inflammatory effect on porcine T cell subpopulations." . . . . "RIV/00027162:_____/12:#0000916" . "Adenosine is a well described anti-inflammatory modulator of immune responses. The aim of the present study was to describe the role of common adenosine agonist 5'-N-ethylcarboxamidoadenosine (NECA) in cytokine production by main porcine T cell subpopulations. TNF-alpha, IFN-gamma, IL-2 and IL-10 were detected by multicolor flow cytometry together with cell surface markers CD3, CD4 and CD8. It was found that NECA inhibits (in a dose-dependent manner) production of pro-inflammatory TNF-alpha and Th1-associated cytokines IFN-gamma, IL-2 in all concanavalin A-stimulated T cell subpopulations. Moreover, production of IL-10 was potentiated in all T cell subpopulations tested. These corresponded well with the fact that all T cell subsets expressed mRNA for adenosine receptor (AR) subtypes to comparable extents. Contrary to concanavalin A-stimulated cells, NECA had a moderate effect on PMA-stimulated T cells, suggesting that AR in pigs acts via signaling pathways not associated with protein-kinase C. Non-selective antagonist CGS15943 as well as allosteric modulator SCH202676 failed to reverse the effect of NECA in pigs. In conclusion, NECA has an anti-inflammatory effect on porcine T cell subpopulations."@en . "The effect of adenosine on pro-inflammatory cytokine production by porcine T cells"@en . "000301159200038" . . . "Pig; T cell subpopulations; Cytokines; 5 '-N-ethylcarboxamidoadenosine; Adenosine receptor subtypes"@en . . . "NL - Nizozemsko" . . . "The effect of adenosine on pro-inflammatory cytokine production by porcine T cells" . . . . "10.1016/j.vetimm.2011.12.002" . "145" . "6"^^ . "RIV/00027162:_____/12:#0000916!RIV13-MZE-00027162" . . "8"^^ . "Veterinary Immunology and Immunopathology" . . "Ondr\u00E1\u010Dkov\u00E1, Petra" . . "4"^^ . "The effect of adenosine on pro-inflammatory cytokine production by porcine T cells"@en . . "Kov\u00E1\u0159\u016F, F." . "132914" . "Lev\u00E1, Lenka" . "1-2" . . . "0165-2427" . "[6EEEF0DA6F06]" . . . "Kov\u00E1\u0159\u016F, H." . "Matia\u0161ovic, J\u00E1n" . . "P(ED0006/01/01), P(IAA601680801), Z(MSM0021620849), Z(MZE0002716202)" . "The effect of adenosine on pro-inflammatory cytokine production by porcine T cells" . .