"22" . "V\u00FDvoj konven\u010Dn\u00ED PCR metody pro detekci parci\u00E1ln\u00ED sekvence genu k\u00F3duj\u00EDc\u00EDho alergenn\u00ED protein parvalbumin makrely obecn\u00E9 (Scomber scombrus)"@cs . "C\u00EDlem studie bylo detekovat specifickou sekvenci genu k\u00F3duj\u00EDc\u00EDho alergenn\u00ED protein parvalbumin makrely obecn\u00E9 (Scomber scombrus). Byla vyvinuta konven\u010Dn\u00ED PCR metoda. Byly navr\u017Eeny specifick\u00E9 primery Specificita metody byla testov\u00E1na na vzorc\u00EDch 23 r\u016Fzn\u00FDch druh\u016F ryb. Separace a vizualizace PCR produktu o velikosti 248 bp byla provedena pomoc\u00ED horizont\u00E1ln\u00ED elektrofor\u00E9zy. Citlivost metody byla 10 pg/ul. Bylo vy\u0161et\u0159eno prvn\u00EDch 12 vzork\u016F ryb\u00EDch v\u00FDrobk\u016F z tr\u017En\u00ED s\u00EDt\u011B. Alergenn\u00ED parvalbumin byl detekov\u00E1n u sedmi v\u00FDrobk\u016F."@cs . . "Development of conventional PCR method to detect the partial sequence of gene encoding an allergenic protein parvalbumin of Atlantic mackerel (Scomber scombrus)"@en . "scombroid fish; parvalbumin; allergen; fish products, PCR"@en . "2"^^ . . "3"^^ . . "Maso" . . "V\u00FDvoj konven\u010Dn\u00ED PCR metody pro detekci parci\u00E1ln\u00ED sekvence genu k\u00F3duj\u00EDc\u00EDho alergenn\u00ED protein parvalbumin makrely obecn\u00E9 (Scomber scombrus)" . "CZ - \u010Cesk\u00E1 republika" . "C\u00EDlem studie bylo detekovat specifickou sekvenci genu k\u00F3duj\u00EDc\u00EDho alergenn\u00ED protein parvalbumin makrely obecn\u00E9 (Scomber scombrus). Byla vyvinuta konven\u010Dn\u00ED PCR metoda. Byly navr\u017Eeny specifick\u00E9 primery Specificita metody byla testov\u00E1na na vzorc\u00EDch 23 r\u016Fzn\u00FDch druh\u016F ryb. Separace a vizualizace PCR produktu o velikosti 248 bp byla provedena pomoc\u00ED horizont\u00E1ln\u00ED elektrofor\u00E9zy. Citlivost metody byla 10 pg/ul. Bylo vy\u0161et\u0159eno prvn\u00EDch 12 vzork\u016F ryb\u00EDch v\u00FDrobk\u016F z tr\u017En\u00ED s\u00EDt\u011B. Alergenn\u00ED parvalbumin byl detekov\u00E1n u sedmi v\u00FDrobk\u016F." . "The aim of this study was to develop the conventional PCR method to detect the major allergenic protein parvalbumin beta of Atlantic mackerel (Scomber scombrus). The specific set of primers for the amplification of the partial genomic sequence of the parvalbumin gene (248 bp) was designed and applied to the investigation of first twelve commercial fish products with Atlantic mackerel declaration. The specificity of the designed primer pair using 23 various fish species was tested. The intrinsic detection limit was 10 pg/ul of the present specific DNA. Atlantic mackerel allergenic parvalbumin was detected in seven food products only."@en . . . "Tremlov\u00E1, B." . "V\u00FDvoj konven\u010Dn\u00ED PCR metody pro detekci parci\u00E1ln\u00ED sekvence genu k\u00F3duj\u00EDc\u00EDho alergenn\u00ED protein parvalbumin makrely obecn\u00E9 (Scomber scombrus)"@cs . . . "RIV/00027162:_____/11:#0000834!RIV12-MZE-00027162" . . . . "Ren\u010Dov\u00E1, Eva" . "V\u00FDvoj konven\u010Dn\u00ED PCR metody pro detekci parci\u00E1ln\u00ED sekvence genu k\u00F3duj\u00EDc\u00EDho alergenn\u00ED protein parvalbumin makrely obecn\u00E9 (Scomber scombrus)" . . . "5" . "3"^^ . "[4B2760BFD7AB]" . . . . "Development of conventional PCR method to detect the partial sequence of gene encoding an allergenic protein parvalbumin of Atlantic mackerel (Scomber scombrus)"@en . "240871" . . . "Z(MZE0002716202)" . . "RIV/00027162:_____/11:#0000834" . "1210-4086" . "Kosteln\u00EDkov\u00E1, Darina" .