. . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Matia\u0161ovic, J\u00E1n" . "\u0160t\u011Bp\u00E1nov\u00E1, Hana" . . . . . . "Pavlov\u00E1, Barbora" . . . "RIV/00027162:_____/11:#0000751" . . "Ondr\u00E1\u010Dkov\u00E1, Petra" . "10.1186/1297-9716-42-16" . . . . "NITRIC-OXIDE PRODUCTION; SEROVAR TYPHIMURIUM; PATHOGENICITY ISLANDS; SPI-1 GENES; CELL-DEATH; APOPTOSIS; INVASION; SIPB; ENTERITIDIS; CASPASE-1"@en . "P(1B44020), P(ED0006/01/01), P(GA524/08/1606), Z(MZE0002716202)" . "0928-4249" . . . . "Volf, Ji\u0159\u00ED" . "Karasov\u00E1, Daniela" . "[A20432429C61]" . . . "SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression" . "Veterinary Research" . . "000290659600005" . . . "Genes localized at Salmonella pathogenicity island-1 (SPI-1) are involved in Salmonella invasion of host non-professional phagocytes. In addition to invasion, SPI-1-encoded proteins induce cell death of macrophages via activation of caspase-1 which also cleaves proIL-1 beta and proIL-18, precursors of 2 proinflammatory cytokines. We were therefore interested in whether SPI-1-encoded type III secretion system (T3SS) may influence proinflammatory response of macrophages. We infected primary porcine alveolar macrophages with wild-type S. Typhimurium and S. Enteritidis and their SPI-1 deletion mutants. SPI1 mutants of both serovars invaded approx. 5 times less efficiently than the wild-type strains and despite this, macrophages responded to the infection with Delta SPI1 mutants by increased expression of proinflammatory cytokines. Our results showed that the SPI-1-encoded T3SS is required not only for cell invasion but in macrophages also for the suppression of early proinflammatory cytokine expression." . . . "Faldyna, Martin" . "16" . . . . . "9"^^ . . "9"^^ . . . "SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression" . . "Crh\u00E1nov\u00E1, Magdal\u00E9na" . . "231474" . "RIV/00027162:_____/11:#0000751!RIV12-MZE-00027162" . "6"^^ . "SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression"@en . "SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression"@en . "Rychl\u00EDk, Ivan" . "Genes localized at Salmonella pathogenicity island-1 (SPI-1) are involved in Salmonella invasion of host non-professional phagocytes. In addition to invasion, SPI-1-encoded proteins induce cell death of macrophages via activation of caspase-1 which also cleaves proIL-1 beta and proIL-18, precursors of 2 proinflammatory cytokines. We were therefore interested in whether SPI-1-encoded type III secretion system (T3SS) may influence proinflammatory response of macrophages. We infected primary porcine alveolar macrophages with wild-type S. Typhimurium and S. Enteritidis and their SPI-1 deletion mutants. SPI1 mutants of both serovars invaded approx. 5 times less efficiently than the wild-type strains and despite this, macrophages responded to the infection with Delta SPI1 mutants by increased expression of proinflammatory cytokines. Our results showed that the SPI-1-encoded T3SS is required not only for cell invasion but in macrophages also for the suppression of early proinflammatory cytokine expression."@en . "42" . . . .