. . "We analysed porcine antigen-specific immunity six months after experimental infection with Mycobacterium avium subsp. avium (MAA) by measuring IFN-gamma production (ELISA, qRT-PCR and flow cytometry) and lymphocyte transformation test after in vitro re-stimulation with the MAA-derived antigen. In MAA-infected animals, IFN-gamma was increased at both mRNA and protein levels in response to the specific MAA antigen in the blood, spleen and mesenteric lymph nodes. Similarly, a positive antigen-driven response was detected by proliferation assay. Using flow cytometry, we found double-positive CD4+CD8+ lymphocytes as the major T lymphocyte subset producing IFN-gamma after specific re-stimulation. In conclusion, antigen-specific immune response in swine is retained for at least six months after infection with MAA. Furthermore, we identified the presence of antigen-specific double-positive CD4+CD8+ cells in MAA-infected pigs."@en . . "Kaevska, Marija" . . . . . . "\u0160tromerov\u00E1, Nikola" . . . . . "P(1B53009), P(ED0006/01/01), Z(MZE0002716202)" . "\u0160t\u011Bp\u00E1nov\u00E1, Hana" . . "NL - Nizozemsko" . "RIV/00027162:_____/11:#0000744" . . "pig; avian tuberculosis; interferon-gamma; CD4+CD8+ T cell"@en . "Pavl\u00EDk, Ivo" . . "Kaevska, Marija" . "Matia\u0161ovic, J\u00E1n" . "Faldyna, Martin" . "Veterinary Immunology and Immunopathology" . . . "189382" . "Cell-mediated immune response in swine infected with Mycobacterium avium subsp. avium"@en . . . . "6"^^ . "1-2" . . "Pavlov\u00E1, Barbora" . "7"^^ . "[EBE78E0575D1]" . "000292486400013" . "Cell-mediated immune response in swine infected with Mycobacterium avium subsp. avium"@en . "0165-2427" . "Cell-mediated immune response in swine infected with Mycobacterium avium subsp. avium" . "7"^^ . . "142" . . "RIV/00027162:_____/11:#0000744!RIV12-MZE-00027162" . . "We analysed porcine antigen-specific immunity six months after experimental infection with Mycobacterium avium subsp. avium (MAA) by measuring IFN-gamma production (ELISA, qRT-PCR and flow cytometry) and lymphocyte transformation test after in vitro re-stimulation with the MAA-derived antigen. In MAA-infected animals, IFN-gamma was increased at both mRNA and protein levels in response to the specific MAA antigen in the blood, spleen and mesenteric lymph nodes. Similarly, a positive antigen-driven response was detected by proliferation assay. Using flow cytometry, we found double-positive CD4+CD8+ lymphocytes as the major T lymphocyte subset producing IFN-gamma after specific re-stimulation. In conclusion, antigen-specific immune response in swine is retained for at least six months after infection with MAA. Furthermore, we identified the presence of antigen-specific double-positive CD4+CD8+ cells in MAA-infected pigs." . "Cell-mediated immune response in swine infected with Mycobacterium avium subsp. avium" . .