. "Pavl\u00EDk, Ivo" . "[C72BD71CEDB8]" . . "Journal of Veterinary Medical Science" . . . "10" . "251871" . "4"^^ . . "RIV/00027162:_____/10:#0000695!RIV11-MZE-00027162" . "Kr\u00E1l\u00EDk, Petr" . . "Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations"@en . . . . . . "000284246900005" . "P(ED0006/01/01), P(QH81065), Z(MZE0002716202)" . . "Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations" . "RIV/00027162:_____/10:#0000695" . "The aim of the present study was to study by culture, and a quantitative real-time polymerase chain reaction, the presence of bacteria in consecutive semen, blood, and fecal samples collected from one infected Piedmont breeding bull during a 380 day period. Five out of seven blood samples and all nine semen samples were positive on real-time quantitative polymerase chain reaction with 101 to 102 and 102 to 104 copies of IS900/F57 per ml, respectively. In all, there were 9 fecal culture positive samples with too numerous to count colony forming units and positive real-time quantitiative polymerase chain reaction ranging from 105 to 107 copies of IS900/F57. After the bull was euthanized, Mycobacterium avium subsp. paratuberculosis was cultured from various parts of small and large intestines, liver tissue, lymph nodes, and from epididymis and vesicular glands."@en . . . "72" . . "Breeding bull; Cattle; Johne's disease; paratuberculosis; semen analysis"@en . "Baumgartner, W." . . "0916-7250" . "Khol, J. L." . "JP - Japonsko" . . "Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations"@en . . . "Aurich, C." . . "7"^^ . "The aim of the present study was to study by culture, and a quantitative real-time polymerase chain reaction, the presence of bacteria in consecutive semen, blood, and fecal samples collected from one infected Piedmont breeding bull during a 380 day period. Five out of seven blood samples and all nine semen samples were positive on real-time quantitative polymerase chain reaction with 101 to 102 and 102 to 104 copies of IS900/F57 per ml, respectively. In all, there were 9 fecal culture positive samples with too numerous to count colony forming units and positive real-time quantitiative polymerase chain reaction ranging from 105 to 107 copies of IS900/F57. After the bull was euthanized, Mycobacterium avium subsp. paratuberculosis was cultured from various parts of small and large intestines, liver tissue, lymph nodes, and from epididymis and vesicular glands." . . "Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations" . . "6"^^ . "Beran, Vladim\u00EDr" . . "Slan\u00E1, Iva" .