. . "000281002700011" . . . "197" . . . "260484" . "IE - Irsko" . "Genotoxic polycyclic aromatic hydrocarbons fail to induce the p53-dependent DNA damage response, apoptosis or cell-cycle arrest in human prostate carcinoma LNCaP cells"@en . . "Milcov\u00E1, A." . . . "Vondr\u00E1\u010Dek, Jan" . . "0378-4274" . "Exposure to polycyclic aromatic hydrocarbons has been positively associated with prostate cancer, but knowledge of the formation of PAH-DNA adducts and related genotoxic events in prostatic cells is limited. In the present study, benzo[a]pyrene, formed significant levels of DNA adducts in cell lines derived from human prostate carcinoma. Despite a significant amount of DNA adducts being formed, neither apoptosis nor cell-cycle arrest were induced in LNCaP cells, presumably due to their failure to activate the p53-dependent DNA damage response. LNCaP cells were not sensitized to the induction of apoptosis by PAHs even through inhibition of the PI3K/Akt pro-survival pathway and the lack of apoptosis was not due a disruption of expression of pro-apoptotic and pro-survival members of the Bcl-2 family of apoptosis regulators. In contrast to other genotoxic stimuli, genotoxic PAHs failed to induce DNA double-strand breaks, as illustrated by the lack of phosphorylation of histone H2AX or Chk-2 activation." . "Machala, Miroslav" . . "3" . "Marvanov\u00E1, So\u0148a" . . "Prostate epithelial cells; DNA adducts; Apoptosis; Cell-cycle arrest; p53; Carcinogenesis"@en . "P(GA310/07/0961), Z(AV0Z50040507), Z(AV0Z50040702), Z(AV0Z50390512), Z(AV0Z50390703), Z(MZE0002716202)" . "9"^^ . . "Libalov\u00E1, H." . . "Kr\u010Dm\u00E1\u0159, Pavel" . . "TOXICOLOGY LETTERS" . . "Genotoxic polycyclic aromatic hydrocarbons fail to induce the p53-dependent DNA damage response, apoptosis or cell-cycle arrest in human prostate carcinoma LNCaP cells" . . "Vykopalov\u00E1, Lenka" . . "12"^^ . "Genotoxic polycyclic aromatic hydrocarbons fail to induce the p53-dependent DNA damage response, apoptosis or cell-cycle arrest in human prostate carcinoma LNCaP cells" . "Trilecov\u00E1, Lenka" . "RIV/00027162:_____/10:#0000687" . . "RIV/00027162:_____/10:#0000687!RIV11-GA0-00027162" . . "7"^^ . . . . . "Starsichova, A." . "Hrub\u00E1, Eva" . . . . "Sou\u010Dek, K." . . "Exposure to polycyclic aromatic hydrocarbons has been positively associated with prostate cancer, but knowledge of the formation of PAH-DNA adducts and related genotoxic events in prostatic cells is limited. In the present study, benzo[a]pyrene, formed significant levels of DNA adducts in cell lines derived from human prostate carcinoma. Despite a significant amount of DNA adducts being formed, neither apoptosis nor cell-cycle arrest were induced in LNCaP cells, presumably due to their failure to activate the p53-dependent DNA damage response. LNCaP cells were not sensitized to the induction of apoptosis by PAHs even through inhibition of the PI3K/Akt pro-survival pathway and the lack of apoptosis was not due a disruption of expression of pro-apoptotic and pro-survival members of the Bcl-2 family of apoptosis regulators. In contrast to other genotoxic stimuli, genotoxic PAHs failed to induce DNA double-strand breaks, as illustrated by the lack of phosphorylation of histone H2AX or Chk-2 activation."@en . "Genotoxic polycyclic aromatic hydrocarbons fail to induce the p53-dependent DNA damage response, apoptosis or cell-cycle arrest in human prostate carcinoma LNCaP cells"@en . "[6C39710969EB]" . . "Topinka, J." . .