"0168-1605" . . . "Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment" . . . "141" . . . "Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment" . "Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment"@en . . "[81788D5FA23C]" . "000281830700010" . "3"^^ . . . "273323" . "7"^^ . . . . "Pavl\u00EDk, Ivo" . "2"^^ . . . "We have evaluated a promising technology combining sample treatment with propidium monoazide (PMA) prior to DNA extraction for selective detection of cells with intact cell membranes with detection of sequence element F57 by quantitative PCR (F57 qPCR). Element F57 is unique for MAP and is not known to exist in any other bacterial species. Conditions of PMA treatment were optimised for MAP isolate 7082 using live and heat-killed cells and comparing different DNA extraction procedures. The subsequent successful application of the optimised protocol to four other MAP isolates of different origins suggested that the optimised protocol might be broadly applicable to different MAP strains. Furthermore, different equations were compared to use the data resulting from this technology to optimally predict the percentage of live MAP cells in mixtures containing both live and dead cells." . . "RIV/00027162:_____/10:#0000633" . "International Journal of Food Microbiology" . "RIV/00027162:_____/10:#0000633!RIV11-MZE-00027162" . "We have evaluated a promising technology combining sample treatment with propidium monoazide (PMA) prior to DNA extraction for selective detection of cells with intact cell membranes with detection of sequence element F57 by quantitative PCR (F57 qPCR). Element F57 is unique for MAP and is not known to exist in any other bacterial species. Conditions of PMA treatment were optimised for MAP isolate 7082 using live and heat-killed cells and comparing different DNA extraction procedures. The subsequent successful application of the optimised protocol to four other MAP isolates of different origins suggested that the optimised protocol might be broadly applicable to different MAP strains. Furthermore, different equations were compared to use the data resulting from this technology to optimally predict the percentage of live MAP cells in mixtures containing both live and dead cells."@en . "P(ED0006/01/01), P(QH81065), Z(MZE0002716202)" . "Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment"@en . . "Suppl 1" . . . "NL - Nizozemsko" . "Kr\u00E1l\u00EDk, Petr" . . "Nocker, A." . . "MAP; Johne's disease; PMA; Paratuberculosis; Crohn's disease"@en . .