"Pia\u010Dkov\u00E1, V." . "253636" . "V\u00EDcenov\u00E1, Monika" . "RIV/00027162:_____/10:#0000611" . "Reschov\u00E1, Stanislava" . . . . . "5" . "0893-8849" . "Journal of the World Aquaculture Society" . . . "000282697900011" . "Cyprinid herpesvirus-3(CyHV-3) is a causative agent of the lethal disease in koi and common carp that can adversely affect common and koi carp breeds.At present, several different PCR tests are in use.In this study, we compared the sensitivity of the single-round and nested PCR assays by testing the panel of field tissue samples(106) of koi and common carp and by testing tissue samples with known amounts of CyHV-3 DNA. The sensitivity of all the PCR methods was similar within testing the spiked homogenate sample with a known amount of CyHV-3 DNA (ca.12 DNA copies), with the exception of Gray PCR (25 copies). Detection levels of the individual PCR methods in field samples varied.The most sensitive and suitable seems to be two-round Bercovier method, detection of 44,3% of positive samples in total." . . "Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods"@en . . . . "41" . . . "Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods" . "[AE39D07E5084]" . . . . "RIV/00027162:_____/10:#0000611!RIV11-MZE-00027162" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "6"^^ . "P(QH71057), Z(MSM6007665809), Z(MZE0002716201), Z(MZE0002716202)" . . . "Koi; Common carp; polymerase chain reaction (PCR)"@en . "Vesel\u00FD, Tom\u00E1\u0161" . . "Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods" . "Pokorov\u00E1, Dagmar" . . "5"^^ . . "7"^^ . "Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods"@en . . "H\u016Flov\u00E1, Jana" . "Cyprinid herpesvirus-3(CyHV-3) is a causative agent of the lethal disease in koi and common carp that can adversely affect common and koi carp breeds.At present, several different PCR tests are in use.In this study, we compared the sensitivity of the single-round and nested PCR assays by testing the panel of field tissue samples(106) of koi and common carp and by testing tissue samples with known amounts of CyHV-3 DNA. The sensitivity of all the PCR methods was similar within testing the spiked homogenate sample with a known amount of CyHV-3 DNA (ca.12 DNA copies), with the exception of Gray PCR (25 copies). Detection levels of the individual PCR methods in field samples varied.The most sensitive and suitable seems to be two-round Bercovier method, detection of 44,3% of positive samples in total."@en . . .