. "8"^^ . . . "Veterinary Research" . . "The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization" . "RIV/00027162:_____/09:#0000551!RIV10-MZE-00027162" . "The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization"@en . "0928-4249" . . . "Ducatelle, R." . "Boyen, F." . . "328159" . . "1"^^ . "000272039500013" . . "FR - Francouzsk\u00E1 republika" . . "[5B6EB937EEF4]" . . "The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization" . "This study aimed at examining the interaction between mycotoxin deoxynivalenol (DON) and Salmonella Typhimurium at the level of the porcine innate immune system, represented by macrophages. First, we assessed the direct cytotoxic effect of DON on porcine macrophages. Incubation with 0.25 ug/ml of DON or higher resulted in a significant cytotoxic effect after 24 h of incubation. Secondly, the direct toxic effect of DON on the growth and on the expression of Salmonella pathogenicity island 1 (SPI-1) and SPI-2 virulence genes of S. Typhimurium was determined. At low non-cytotoxic concentrations, DON was of no effect on growth or virulence gene expression of S. Typhimurium. However, when the invasion and intracellular survival of S. Typhimurium in macrophages preexposed to 0.025 ug/ml of DON was examined, DON significantly promoted the uptake of S. Typhimurium into macrophages which coincided with F-actin reorganization due to the activation of ERK1/2." . "Pasmans, F." . "De Backer, P." . . "Croubels, S." . "RIV/00027162:_____/09:#0000551" . "The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization"@en . "This study aimed at examining the interaction between mycotoxin deoxynivalenol (DON) and Salmonella Typhimurium at the level of the porcine innate immune system, represented by macrophages. First, we assessed the direct cytotoxic effect of DON on porcine macrophages. Incubation with 0.25 ug/ml of DON or higher resulted in a significant cytotoxic effect after 24 h of incubation. Secondly, the direct toxic effect of DON on the growth and on the expression of Salmonella pathogenicity island 1 (SPI-1) and SPI-2 virulence genes of S. Typhimurium was determined. At low non-cytotoxic concentrations, DON was of no effect on growth or virulence gene expression of S. Typhimurium. However, when the invasion and intracellular survival of S. Typhimurium in macrophages preexposed to 0.025 ug/ml of DON was examined, DON significantly promoted the uptake of S. Typhimurium into macrophages which coincided with F-actin reorganization due to the activation of ERK1/2."@en . "Z(MZE0002716201)" . . "Haesebrouck, F." . . . . "6" . "deoxynivalenol; Salmonella Typhimurium; pig; macrophage; cytoskeleton"@en . "12"^^ . "40" . "Rychl\u00EDk, Ivan" . . "Vandenbroucke, V." .