. "Szmolka, A." . . . . "BMC Microbiology" . . "348966" . "Virulence potential of five major pathogenicity islands (SPI-1 to SPI-5) of Salmonella enterica serovar Enteritidis for chickens" . "10"^^ . . "Virulence potential of five major pathogenicity islands (SPI-1 to SPI-5) of Salmonella enterica serovar Enteritidis for chickens" . . "Virulence potential of five major pathogenicity islands (SPI-1 to SPI-5) of Salmonella enterica serovar Enteritidis for chickens"@en . "\u0160i\u0161\u00E1k, Franti\u0161ek" . . "RIV/00027162:_____/09:#0000546!RIV10-MZE-00027162" . "Virulence potential of five major pathogenicity islands (SPI-1 to SPI-5) of Salmonella enterica serovar Enteritidis for chickens"@en . . . . . "[952851B57C78]" . "\u0160ebkov\u00E1, Alena" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "9" . . "Salmonella; pathogenicity island; chicken; virulence"@en . . "Ability Salmonella to infect and colonise a broad range of hosts coincided with the introduction of 5 major pathogenicity islands (SPI1-5) into the Salmonella genome. However, only limited information is available on how each of these pathogenicity islands influences the ability of Salmonella to infect chickens. In this study, we therefore constructed Salmonella Enteritidis mutants with each SPI deleted separately, with single individual SPIs (i.e. with the remaining four deleted) and a mutant with all 5 SPIs deleted, and assessed their virulence in one-day-old chickens. The mutant lacking all 5 major SPIs was still capable of colonising the caecum while colonisation of the liver and spleen was dependent on the presence of both SPI-1 and SPI-2. In contrast, the absence of SPI-3, SPI-4 or SPI-5 individually did not influence virulence of S. Enteritidis for chickens, but collectively they contributed to the colonisation of the spleen." . . "1471-2180" . . "Kummer, Vladim\u00EDr" . "P(GA524/09/0215), Z(MZE0002716201)" . "Karasov\u00E1, Daniela" . . . "Ability Salmonella to infect and colonise a broad range of hosts coincided with the introduction of 5 major pathogenicity islands (SPI1-5) into the Salmonella genome. However, only limited information is available on how each of these pathogenicity islands influences the ability of Salmonella to infect chickens. In this study, we therefore constructed Salmonella Enteritidis mutants with each SPI deleted separately, with single individual SPIs (i.e. with the remaining four deleted) and a mutant with all 5 SPIs deleted, and assessed their virulence in one-day-old chickens. The mutant lacking all 5 major SPIs was still capable of colonising the caecum while colonisation of the liver and spleen was dependent on the presence of both SPI-1 and SPI-2. In contrast, the absence of SPI-3, SPI-4 or SPI-5 individually did not influence virulence of S. Enteritidis for chickens, but collectively they contributed to the colonisation of the spleen."@en . "9"^^ . . . . "RIV/00027162:_____/09:#0000546" . "1" . . "Havl\u00ED\u010Dkov\u00E1, Hana" . "Volf, Ji\u0159\u00ED" . "Nagy, B." . "Imre, A." . . "7"^^ . "Rychl\u00EDk, Ivan" . .