. "7"^^ . "Vondr\u00E1\u010Dek, Jan" . "2" . "000265196900018" . . "Poellinger, L." . . "Pastorek, J." . "[DC237072E4E7]" . . . "Role of aryl hydrocarbon receptor in modulation of the expression of the hypoxia marker carbonic anhydrase IX" . . "Biochemical Journal" . "339690" . "Tumour-associated expression of CA IX (carbonic anhydrase IX) is to a major extent regulated by HIF-1 (hypoxia-inducible factor-1) which is important for transcriptional activation and consists of the oxygen-regulated subunit HIF-1 alpha and the partner factor ARNT (AhR nuclear translocator). We have previously observed that HIF-1 alpha competes with the AhR for interaction with ARNT under conditions when both factors are activated. We have therefore investigated whether TCDD-induced activation of the AhR pathway might interfere with CA IX expression. The results from the present study suggest that TCDD treatment reduces hypoxic induction of both CA IX mRNA and protein expression. Moreover, the transcriptional activity of the CA9 promoter was significantly reduced by expression of CAAhR (Constitutively active AhR), which activates, transcription in a ligand-independent manner. Finally, we found that ARNT is critical for both hypoxic induction and the TCDD-mediated inhibition of CA9 expression."@en . "Role of aryl hydrocarbon receptor in modulation of the expression of the hypoxia marker carbonic anhydrase IX"@en . . "Z(AV0Z50040507), Z(AV0Z50040702), Z(MZE0002716201)" . "Pastorekov\u00E1, S." . . . . "ARNT TRANSCRIPTION FACTOR; INDUCIBLE FACTOR-I; DIOXIN RECEPTOR; SIGNALING PATHWAYS; GENE-EXPRESSION; AH RECEPTOR; PAS DOMAIN; CROSS-TALK; IDENTIFICATION; PROTEIN"@en . . . "10"^^ . "P\u011Bn\u010D\u00EDkov\u00E1, Kate\u0159ina" . "RIV/00027162:_____/09:#0000540!RIV10-MZE-00027162" . . . "0264-6021" . . . . "3"^^ . "Machala, Miroslav" . . "Gradin, K." . "Tak\u00E1\u010Dov\u00E1, M." . . "Tumour-associated expression of CA IX (carbonic anhydrase IX) is to a major extent regulated by HIF-1 (hypoxia-inducible factor-1) which is important for transcriptional activation and consists of the oxygen-regulated subunit HIF-1 alpha and the partner factor ARNT (AhR nuclear translocator). We have previously observed that HIF-1 alpha competes with the AhR for interaction with ARNT under conditions when both factors are activated. We have therefore investigated whether TCDD-induced activation of the AhR pathway might interfere with CA IX expression. The results from the present study suggest that TCDD treatment reduces hypoxic induction of both CA IX mRNA and protein expression. Moreover, the transcriptional activity of the CA9 promoter was significantly reduced by expression of CAAhR (Constitutively active AhR), which activates, transcription in a ligand-independent manner. Finally, we found that ARNT is critical for both hypoxic induction and the TCDD-mediated inhibition of CA9 expression." . . . "Role of aryl hydrocarbon receptor in modulation of the expression of the hypoxia marker carbonic anhydrase IX" . . "Kop\u00E1\u010Dek, J." . "Role of aryl hydrocarbon receptor in modulation of the expression of the hypoxia marker carbonic anhydrase IX"@en . . "RIV/00027162:_____/09:#0000540" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . . "Holotnakova, T." . . "419" .