"Dalmo, R." . . "13"^^ . "R" . . "1"^^ . . "Rainbow trout; VHSV; DNA immunization; cell-mediated cytotoxicity; homing"@en . "RIV/00027162:_____/08:#0000430!RIV09-MZE-00027162" . "32" . "3" . "Bergmann, S." . "Vesel\u00FD, Tom\u00E1\u0161" . "Bu\u0148kami zprost\u0159edkovan\u00E1 imunitn\u00ED odpov\u011B\u010F u pstruha duhov\u00E9ho po DNA imunizaci proti viru virov\u00E9 hemoragick\u00E9 septik\u00E9mie"@cs . . "Cell-mediated immune responses in rainbow trout after DNA immunization against the viral hemorrhagic septicemia virus"@en . "Fischer, U." . "Kock, H." . "Ototake, M." . . "0145-305X" . . "Cell-mediated immune responses in rainbow trout after DNA immunization against the viral hemorrhagic septicemia virus"@en . "RIV/00027162:_____/08:#0000430" . . . "Pstruh duhov\u00FD byl imunizov\u00E1n DNA vektory k\u00F3duj\u00EDc\u00EDmi G a N protein VHSV, aby bylo zji\u0161t\u011Bno, kter\u00FD z nich indukuje bu\u0148kami zprost\u0159edkovanou cytotoxicitu (CMC) proti viru virov\u00E9 hemoragick\u00E9 septik\u00E9mie (VHSV). G protein byl u\u010Dinn\u011Bj\u0161\u00ED ne\u017E N protein. Leukocyty perifern\u00ED krve (PBL) izolovan\u00E9 ze pstruha imunizovan\u00E9ho proti G proteinu zab\u00EDjely infikovan\u00E9 RTG-2 bu\u0148ky i xenogenn\u00ED EPC bu\u0148ky, co\u017E nazna\u010Duje \u00FA\u010Dast cytotoxick\u00FDch T lymfocyt\u016F i NK bun\u011Bk. Oproti tomu, PBL ze ptruha imunizovan\u00E9ho proti N proteinu zab\u00EDjely pouze infikovan\u00E9 RTG-2 bu\u0148ky, co\u017E nazna\u010Duje, \u017Ee tento protein vyvol\u00E1v\u00E1 pouze CTL odpov\u011B\u010F. Toto je prvn\u00ED zpr\u00E1va o cytotoxick\u00E9 imunitn\u00ED odpov\u011Bdi u ryb po DNA vakcinaci."@cs . "Lorenzen, N." . "Einer-Jensen, K." . "To identify viral proteins that induce cell-mediated cytotoxicity (CMC) against viral hemorrhagic septicemia virus (VHSV)-infected cells, rainbow trout were immunized with DNA vectors encoding the glycoprotein G or the nucleocapsid protein N of VHSV. The G protein was a more potent trigger of cytotoxic cells than the N protein. Peripheral blood leukocytes (PBL) isolated from trout immunized against the G protein killed both VHSVinfected MHC class I matched (RTG-2) and VHSV-infected xenogeneic (EPC) target cells, suggesting the involvement of both cytotoxic T lymphocytes (CTL) and NK cells, respectively. In contrast, PBL from trout that were immunized against the N protein only killed VHSV-infected RTG-2 cells, indicating that this protein only elicits a CTL response. This is the first report on cytotoxic immune responses after DNA vaccination in fish." . "Developmental and Comparative Immunology" . . "000252651500008" . . . "K\u00F6llner, B." . "359049" . "Schuetze, H." . "[0B513C2FF412]" . "11"^^ . "Cell-mediated immune responses in rainbow trout after DNA immunization against the viral hemorrhagic septicemia virus" . . "Bu\u0148kami zprost\u0159edkovan\u00E1 imunitn\u00ED odpov\u011B\u010F u pstruha duhov\u00E9ho po DNA imunizaci proti viru virov\u00E9 hemoragick\u00E9 septik\u00E9mie"@cs . "Cell-mediated immune responses in rainbow trout after DNA immunization against the viral hemorrhagic septicemia virus" . . "Utke, K." . "To identify viral proteins that induce cell-mediated cytotoxicity (CMC) against viral hemorrhagic septicemia virus (VHSV)-infected cells, rainbow trout were immunized with DNA vectors encoding the glycoprotein G or the nucleocapsid protein N of VHSV. The G protein was a more potent trigger of cytotoxic cells than the N protein. Peripheral blood leukocytes (PBL) isolated from trout immunized against the G protein killed both VHSVinfected MHC class I matched (RTG-2) and VHSV-infected xenogeneic (EPC) target cells, suggesting the involvement of both cytotoxic T lymphocytes (CTL) and NK cells, respectively. In contrast, PBL from trout that were immunized against the N protein only killed VHSV-infected RTG-2 cells, indicating that this protein only elicits a CTL response. This is the first report on cytotoxic immune responses after DNA vaccination in fish."@en . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . .