. "RIV/00027162:_____/08:#0000344!RIV09-MZE-00027162" . . "Dexamethasone-induced immunosuppression: a rabbit model" . "Lev\u00E1, Lenka" . "RIV/00027162:_____/08:#0000344" . "000255325600004" . . . "P(GA524/05/2714), Z(MZE0002716201)" . . . . "The aim of this study was to characterize a model of immunosuppression in rabbits, based on changes in the lymphocyte subset distribution and changes in proliferative capacity of lymphocytes 1, 3 and 7 days after the administration of 2 mg/kg dexamethasone phosphate (DXP) three times at 6-h intervals. In blood, neutrophilia and lymphopenia without leukocytosis was detected. One day after DXP administration the absolute numbers of all lymphocyte subsets decreased in the blood, whereas in bone marrow, absolute numbers of all lymphocyte subsets increased significantly. In the thymus, DXP led to a marked reduction of numbers of CD4+CD8+ thymocytes. The proliferative capacity of lymphocytes was lower in the blood and spleen only on day 1, no changes were detected in lymph nodes or in bone marrow. The results demonstrate that this DXP application protocol is useful for the experimental induction of short-lasting immunosuppression in rabbits." . "Veterinary Immunology and Immunopathology" . "10"^^ . . . . "Glucocorticoid; Lymphocyte subsets; Lagomorphs; Flow cytometry"@en . . "NL - Nizozemsko" . . "Dexamethasone-induced immunosuppression: a rabbit model" . "Dexamethazonem indukovan\u00E1 imunosuprese - kr\u00E1li\u010D\u00ED model"@cs . "363193" . . "Dexamethazonem indukovan\u00E1 imunosuprese - kr\u00E1li\u010D\u00ED model"@cs . . "Dexamethasone-induced immunosuppression: a rabbit model"@en . "C\u00EDlem studie bylo charakterizovat model imunosuprese u kr\u00E1l\u00EDka na z\u00E1klad\u011B zm\u011Bn v distribuci subpopulac\u00ED lymfocyt\u016F a zm\u011Bn v jejich prolifera\u010Dn\u00ED kapacit\u011B 1, 3 a 7 dn\u00ED po aplikaci 2 mg/kg dexamethazon-fosf\u00E1tu (DXP) t\u0159ikr\u00E1t v 6-hodinov\u00FDch intervalech. V krvi byla detekov\u00E1na neutrofilie a lymfopenie bez leukocyt\u00F3zy. Jeden den po aplikaci DXP, v\u0161echny subpopulace lymfocyt\u016F klesly v krvi, zat\u00EDmco v kostn\u00ED d\u0159eni se absolutn\u00ED po\u010Det v\u0161ech lymfocyt\u00E1rn\u00EDch subpopulac\u00ED signifikantn\u011B zv\u00FD\u0161il, krom\u011B CD79alfa+ bun\u011Bk, jejich\u017E po\u010Det stoupl pouze relativn\u011B. Efekt DXP na lymfocyty ze sleziny a mezenteri\u00E1ln\u00ED a poplite\u00E1ln\u00ED m\u00EDzn\u00ED uzlinu nebyl tak v\u00FDrazn\u00FD. V thymu, DXP vyvolal v\u00FDraznou redukci relativn\u00EDho i absolutn\u00EDho po\u010Dtu CD4+CD8+ thymocyt\u016F. Prolifera\u010Dn\u00ED kapacita lymfocyt\u016F byla ni\u017E\u0161\u00ED pouze prvn\u00ED den v krvi a slezin\u011B, \u017E\u00E1dn\u00E9 zm\u011Bny nebyly detekov\u00E1ny v m\u00EDzn\u00EDch uzlin\u00E1ch a kostn\u00ED d\u0159eni. V\u00FDsledky ukazuj\u00ED, \u017Ee takov\u00FDto protokol aplikace DXP je pou\u017Eiteln\u00FD pro experiment\u00E1ln\u00ED indukci kr\u00E1tkodob\u00E9 imunosuprese u kr\u00E1l\u00EDka."@cs . . "[175E7546002A]" . "4"^^ . . "0165-2427" . "Jeklov\u00E1, Edita" . "Faldyna, Martin" . . "Jagli\u010D, Zoran" . "The aim of this study was to characterize a model of immunosuppression in rabbits, based on changes in the lymphocyte subset distribution and changes in proliferative capacity of lymphocytes 1, 3 and 7 days after the administration of 2 mg/kg dexamethasone phosphate (DXP) three times at 6-h intervals. In blood, neutrophilia and lymphopenia without leukocytosis was detected. One day after DXP administration the absolute numbers of all lymphocyte subsets decreased in the blood, whereas in bone marrow, absolute numbers of all lymphocyte subsets increased significantly. In the thymus, DXP led to a marked reduction of numbers of CD4+CD8+ thymocytes. The proliferative capacity of lymphocytes was lower in the blood and spleen only on day 1, no changes were detected in lymph nodes or in bone marrow. The results demonstrate that this DXP application protocol is useful for the experimental induction of short-lasting immunosuppression in rabbits."@en . "3-4" . "4"^^ . . . "Dexamethasone-induced immunosuppression: a rabbit model"@en . . "122" . .