. "0034-5288" . . "000258188300010" . "Strategy for the detection and differentiation of Mycobacterium avium species in isolates and heavily infected tissues" . "The members of Mycobacterium avium species, comprising M. a. subsp. paratuberculosis (MAP), M. a. hominissuis (MAH), M. a. avium (MAA), M. a. silvaticum (MAS), are currently the most prevalent opportunistic pathogenic mycobacteria causing mycobacterial infection in animals and humans. The ability to distinguish between these subspecies is of relevance for proper diagnosis and control programmes of the diseases. The aim of this study was to design a fast and specific PCR strategy for the detection and differentiation of M. avium subsp. from the solid plate cultures for use in routine veterinary diagnosis. We have developed a multiplex PCR based on IS900, IS901, IS1245 and the dnaJ gene. This method allows the detection of MAP, MAH and MAA/ MAS in one PCR reaction and theoretically enables mixed infections of MAP and MAA or MAP and MAH to be revealed. The sensitivity of this multiplex PCR 1000 CFU enabled the use of this test directly for DNA isolated from the tissue of the heavily infected sheep."@en . . "Mor\u00E1vkov\u00E1, Monika" . . "CZ - \u010Cesk\u00E1 republika" . "Cuteri, V." . "Strategie pro detekci a diferenciaci druh\u016F Mycobacterium avium u izol\u00E1t\u016F a siln\u011B zasa\u017Een\u00FDch tk\u00E1n\u00ED"@cs . "8"^^ . . . "2" . "Hlo\u017Eek, P." . "Druh Mycobacterium avium, obsahuj\u00EDc\u00ED poddruhy M. a. subsp. paratuberculosis (MAP), M. a. hominissuis (MAH), M. a. avium (MAA), M. a. silvaticum (MAS), je v sou\u010Dasn\u00E9 dob\u011B jeden z nejv\u00EDce roz\u0161\u00ED\u0159en\u00FDch oportunn\u00EDch patogen\u016F infikuj\u00EDc\u00EDch jak zv\u00ED\u0159ata tak lidi. Rozli\u0161en\u00ED jednotliv\u00FDch z\u00E1stupc\u016F je d\u016Fle\u017Eit\u00E9 jak z hlediska spr\u00E1vn\u00E9 diagnostiky, tak p\u0159i p\u0159\u00EDprav\u011B preventivn\u00EDch program\u016F. C\u00EDlem t\u00E9to studie bylo navrhnout rychlou a specifickou PCR pro detekci a rozli\u0161en\u00ED jednotliv\u00FDch z\u00E1stupc\u016F z ji\u017E narostl\u00FDch bakteri\u00E1ln\u00EDch kultur. Proto jsme navrhli multiplex PCR, kter\u00FD vyu\u017E\u00EDv\u00E1 specifick\u00FDch inzer\u010Dn\u00EDch sekvenc\u00ED IS900, IS901, IS1245 a genu dnaJ. Metoda tak umo\u017E\u0148uje detekci MAP, MAH a MAA/ MAS v jedn\u00E9 PCR reakci a teoreticky umo\u017E\u0148uje detekovat mix infekce MAP a MAA nebo MAP a MAH. Citlivost t\u00E9to metody je kolem 1000 CFU, co\u017E \u010Din\u00ED tuto metodou vyu\u017Eitelnou t\u00E9\u017E pro p\u0159\u00EDm\u00E9 detekce jednotliv\u00FDch z\u00E1stupc\u016F z masivn\u011B infikovan\u00FDch tk\u00E1n\u00ED. V t\u00E9to studii jsem navr\u017Eenou metodu pou\u017Eili na detekci MAP ze tk\u00E1n\u00ED ovc\u00ED."@cs . "397624" . "RIV/00027162:_____/08:#0000341!RIV09-MZE-00027162" . "Strategy for the detection and differentiation of Mycobacterium avium species in isolates and heavily infected tissues"@en . . . . "Preziuso, S." . . "Research in Veterinary Science" . "[120F792B0CE7]" . . "7"^^ . "85" . . . . . . . "Strategy for the detection and differentiation of Mycobacterium avium species in isolates and heavily infected tissues" . . "Avian tuberculosis; Insertion sequences; Mycobacteriosis; Paratuberculosis; Johne's disease"@en . . "Pavl\u00EDk, Ivo" . "4"^^ . . "The members of Mycobacterium avium species, comprising M. a. subsp. paratuberculosis (MAP), M. a. hominissuis (MAH), M. a. avium (MAA), M. a. silvaticum (MAS), are currently the most prevalent opportunistic pathogenic mycobacteria causing mycobacterial infection in animals and humans. The ability to distinguish between these subspecies is of relevance for proper diagnosis and control programmes of the diseases. The aim of this study was to design a fast and specific PCR strategy for the detection and differentiation of M. avium subsp. from the solid plate cultures for use in routine veterinary diagnosis. We have developed a multiplex PCR based on IS900, IS901, IS1245 and the dnaJ gene. This method allows the detection of MAP, MAH and MAA/ MAS in one PCR reaction and theoretically enables mixed infections of MAP and MAA or MAP and MAH to be revealed. The sensitivity of this multiplex PCR 1000 CFU enabled the use of this test directly for DNA isolated from the tissue of the heavily infected sheep." . "Strategy for the detection and differentiation of Mycobacterium avium species in isolates and heavily infected tissues"@en . "Beran, Vladim\u00EDr" . "Barto\u0161, Milan" . "RIV/00027162:_____/08:#0000341" . . "Strategie pro detekci a diferenciaci druh\u016F Mycobacterium avium u izol\u00E1t\u016F a siln\u011B zasa\u017Een\u00FDch tk\u00E1n\u00ED"@cs . . "P(1B53009), Z(MZE0002716201)" .