"Tumor necrosis factor-alpha modulates effects of aryl hydrocarbon receptor ligands on cell proliferation and expression of cytochrome P450 enzymes in rat liver 'stem-like' cells"@en . . . . . . . "79;89" . . "Machala, Miroslav" . . "Toxicological Sciences" . . . "Vondr\u00E1\u010Dek, Jan" . "1096-6080" . "[B6D11F2E53F2]" . . "R\u016Fzn\u00E9 jatern\u00ED choroby vedou k rozs\u00E1hl\u00E9 z\u00E1n\u011Btliv\u00E9 odpov\u011Bdi a uvoln\u011Bn\u00ED \u0159ady proz\u00E1n\u011Btliv\u00FDch cytokin\u016F, jako je TNF-alfa. Zab\u00FDvali jsme se mo\u017En\u00FDmi interakcemi TNF-alfa s ligandy receptoru AhR a zn\u00E1m\u00FDmi jatern\u00EDmi karcinogeny, jako je 2,3,7,8-TCDD a koplan\u00E1rn\u00ED PCB 126. TNF-alfa v\u00FDznamn\u011B zesiloval prolifera\u010Dn\u00ED \u00FA\u010Dinky pikomol\u00E1rn\u00EDch koncentrac\u00ED TCDD i PCB 126, co\u017E vedlo k n\u00E1rustu po\u010Dtu bun\u011Bk a zv\u00FD\u0161en\u00E9mu procentu bun\u011Bk vstupuj\u00EDc\u00EDch do S-f\u00E1ze bun\u011B\u010Dn\u00E9ho cyklu. Kombinace TNF-alfa s n\u00EDzk\u00FDmi koncentracemi ligand\u016F AhR zvy\u0161ovala hladinu cyklinu A na \u00FArovni mRNA i proteinu. TNF-alfa do\u010Dasn\u011B inhiboval AhR-dependentn\u00ED indukci cytochromu 1A1. Naproti tomu, TNF-alfa v\u00FDznamn\u011B zvy\u0161oval indukci CYP1B1. Tyto v\u00FDsledky nazna\u010Duj\u00ED, \u017Ee TNF-alfa m\u016F\u017Ee v\u00FDznamn\u011B zesilovat \u00FA\u010Dinky ligand\u016F AhR na deregulaci kontroly bun\u011B\u010Dn\u00E9 proliferace, stejn\u011B jako \u00FA\u010Dinky na expresi CYP1B1, kter\u00FD je zahrnut v metabolick\u00E9 aktivaci \u0159ady mutagenn\u00EDch slou\u010Denin."@cs . . "M\u00E1jkov\u00E1, Z." . "TNF-alfa moduluje efekty ligand\u016F receptoru AhR na bun\u011B\u010Dnou proliferaci a expresi enzym\u016F cytochrom\u016F P450 v potkan\u00EDch jatern\u00EDch 'stem-like' bu\u0148k\u00E1ch"@cs . "Z(MZE0002716201)" . "Hennig, B." . "Kozub\u00EDk, A." . . "5"^^ . "11"^^ . "RIV/00027162:_____/07:#0000283" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Tumor necrosis factor-alpha modulates effects of aryl hydrocarbon receptor ligands on cell proliferation and expression of cytochrome P450 enzymes in rat liver 'stem-like' cells" . . "8"^^ . "Tumor necrosis factor-alpha modulates effects of aryl hydrocarbon receptor ligands on cell proliferation and expression of cytochrome P450 enzymes in rat liver 'stem-like' cells" . "cell proliferation; tumor necrosis factor-alpha; aryl hydrocarbon receptor; polychlorinated biphenyl; dioxin; xenobiotic metabolizing enzymes"@en . "Umannov\u00E1, Lenka" . . "Various liver diseases lead to an extensive inflammatory response and release of pro-inflammatory cytokines, such as TNF-alpha. We investigated possible interactions of TNF-alpha with ligands of the AhR receptor and known liver carcinogens, such as 2,3,7,8-TCDD and co-planar PCB 126. TNF-alpha significantly potentiated proliferative effects of picomolar range doses of both TCDD and PCB 126, leading to an increase in cell numbers, as well as an increased percentage of cells entering the S-phase of the cell cycle. The combination of TNF-alpha with low concentrations of AhR ligands increased both mRNA and protein levels of cyclin A. TNF-alpha temporarily inhibited AhR-dependent induction of CYP1A1. In contrast, TNF-alpha significantly enhanced induction of CYP1B1. These results suggest that TNF-alpha can significantly amplify effects of AhR ligands on deregulation of cell proliferation control, as well as on expression of CYP1B1, involved in metabolic activation of a number of mutagenic compounds."@en . "455973" . . "1" . . "Various liver diseases lead to an extensive inflammatory response and release of pro-inflammatory cytokines, such as TNF-alpha. We investigated possible interactions of TNF-alpha with ligands of the AhR receptor and known liver carcinogens, such as 2,3,7,8-TCDD and co-planar PCB 126. TNF-alpha significantly potentiated proliferative effects of picomolar range doses of both TCDD and PCB 126, leading to an increase in cell numbers, as well as an increased percentage of cells entering the S-phase of the cell cycle. The combination of TNF-alpha with low concentrations of AhR ligands increased both mRNA and protein levels of cyclin A. TNF-alpha temporarily inhibited AhR-dependent induction of CYP1A1. In contrast, TNF-alpha significantly enhanced induction of CYP1B1. These results suggest that TNF-alpha can significantly amplify effects of AhR ligands on deregulation of cell proliferation control, as well as on expression of CYP1B1, involved in metabolic activation of a number of mutagenic compounds." . . . . "Tumor necrosis factor-alpha modulates effects of aryl hydrocarbon receptor ligands on cell proliferation and expression of cytochrome P450 enzymes in rat liver 'stem-like' cells"@en . . "Kr\u010Dm\u00E1\u0159, Pavel" . "TNF-alfa moduluje efekty ligand\u016F receptoru AhR na bun\u011B\u010Dnou proliferaci a expresi enzym\u016F cytochrom\u016F P450 v potkan\u00EDch jatern\u00EDch 'stem-like' bu\u0148k\u00E1ch"@cs . "RIV/00027162:_____/07:#0000283!RIV08-MZE-00027162" . "99" . . "Zatloukalov\u00E1, Ji\u0159ina" .