. "414176" . . "Faldyna, Martin" . "We have used selected rabbit anti-human polyclonal antibodies as an example of useful tools for studies on immune system structure and development in veterinary species. The cocktail of anti-human Ig kappa-FITC/anti-Ig lambda-RPE F(ab')2 fragments was used for two-colour and, in combination with the cross-reactive anti-CD79alpha monoclonal antibody HM-57, for three-colour flow cytometry of canine, feline, bovine and porcine peripheral B-cells. Rabbit anti-human lactoferrin-FITC F(ab')2 fragment was used for visualizing neutrophils in dogs, pigs and cattle and an application for two-colour immunophenotyping of canine granulocyte subsets has been designed. Affinity isolated rabbit anti-human CD3 and anti-human TdT have been shown to represent a ready-to-use tool for in situ studies on primary lymphopoiesis. Altogether, our study show that carefully selected polyclonal antibodies available on the market may possess broad cross-reactivity with applications in veterinary research"@en . "Komer\u010Dn\u011B dostupn\u00E9 kr\u00E1l\u00ED\u010D\u00ED polyklon\u00E1ln\u00ED protil\u00E1tky jako n\u00E1stro studi\u00ED imunitn\u00EDho syst\u00E9mu u veterin\u00E1rn\u00EDch druh\u016F"@cs . "6"^^ . "7"^^ . . . . . . "Veterinary Immunology and Immunopathology" . "Ma\u0161kov\u00E1, Jarmila" . . . . "Lev\u00E1, Lenka" . . . "0165-2427" . . . . . . "Kummer, Vladim\u00EDr" . "119" . "\u0160am\u00E1nkov\u00E1, Petra" . "Komer\u010Dn\u011B dostupn\u00E9 kr\u00E1l\u00ED\u010D\u00ED polyklon\u00E1ln\u00ED protil\u00E1tky jako n\u00E1stro studi\u00ED imunitn\u00EDho syst\u00E9mu u veterin\u00E1rn\u00EDch druh\u016F"@cs . "Commercially available rabbit anti-human polyclonal antisera as a useful tool for immune system studies in veterinary species" . "RIV/00027162:_____/07:#0000167" . "NL - Nizozemsko" . "1-2" . "Vybran\u00E9 kr\u00E1l\u00ED\u010D\u00ED polyklon\u00E1ln\u00ED protil\u00E1tky proti lidsk\u00FDm antigen\u016Fm byly pou\u017Eity jako p\u0159\u00EDklad n\u00E1stroje pro studie struktury a v\u00FDvoje imunitn\u00EDho syst\u00E9mu u veterin\u00E1rn\u00EDch druh\u016F. Sm\u011Bs F(ab')2 fragment\u016F protil\u00E1tek proti lidsk\u00E9mu Ig kappa-FITC/anti-Ig lambda-RPE byla pou\u017Eita pro dvoubarevnou a, v kombinaci s k\u0159\u00ED\u017Eov\u011B reaguj\u00EDc\u00ED protil\u00E1tkou HM-57 proti antigenu CD79alfa, trojbarevnou pr\u016Ftokovou cytometrii B lymfocyt\u016F perifern\u00ED krve psa, ko\u010Dky, skotu a prasete. Kr\u00E1li\u010D\u00ED protil\u00E1tka proti lidsk\u00E9mu laktoferinu byla pou\u017Eita pro vizualizaci neutrofiln\u00EDch granulocyt\u016F psa, prasete a skotu a byla nar\u017Eena aplikace dvoubarevn\u00E9 imunofenotypizace ps\u00EDch granulocyt\u016F. D\u00E1le byla prok\u00E1z\u00E1na pou\u017Eitelnost protil\u00E1tky proti CD3 a TdT pro studie lymfopo\u00E9zy. Pr\u00E1ce jako celek dokazuje, \u017Ee pe\u010Dliv\u011B vybran\u00E9 polyklon\u00E1ln\u00ED protil\u00E1tky dostupn\u00E9 na trhu mohou vykazovat \u0161irokou k\u0159\u00ED\u017Eovou reaktivitu a jsou pou\u017Eiteln\u00E9 pro aplikace ve veterin\u00E1rn\u00EDm v\u00FDzkumu."@cs . "Commercially available rabbit anti-human polyclonal antisera as a useful tool for immune system studies in veterinary species" . "Commercially available rabbit anti-human polyclonal antisera as a useful tool for immune system studies in veterinary species"@en . . . "P(GP524/02/P010), Z(MZE0002716201)" . . . . "[7DA2235C30BE]" . "7"^^ . "We have used selected rabbit anti-human polyclonal antibodies as an example of useful tools for studies on immune system structure and development in veterinary species. The cocktail of anti-human Ig kappa-FITC/anti-Ig lambda-RPE F(ab')2 fragments was used for two-colour and, in combination with the cross-reactive anti-CD79alpha monoclonal antibody HM-57, for three-colour flow cytometry of canine, feline, bovine and porcine peripheral B-cells. Rabbit anti-human lactoferrin-FITC F(ab')2 fragment was used for visualizing neutrophils in dogs, pigs and cattle and an application for two-colour immunophenotyping of canine granulocyte subsets has been designed. Affinity isolated rabbit anti-human CD3 and anti-human TdT have been shown to represent a ready-to-use tool for in situ studies on primary lymphopoiesis. Altogether, our study show that carefully selected polyclonal antibodies available on the market may possess broad cross-reactivity with applications in veterinary research" . . "\u0160inkora, J." . "RIV/00027162:_____/07:#0000167!RIV08-MZE-00027162" . . "156;162" . "Canine; Porcine; Bovine; Feline; Lactoferrin; TdT; Immunoglobulin light chain; Flow cytometry; Immunohistochemistry"@en . . . "\u0158eh\u00E1kov\u00E1, Zuzana" . . . "Commercially available rabbit anti-human polyclonal antisera as a useful tool for immune system studies in veterinary species"@en .