. "Immunological and molecular characterization of susceptibility in relationship to bacterial strain differences in Mycobacterium avium subsp. paratuberculosis infection in the red deer (Cervus elaphus)"@en . "6" . . . "Paratuberculosis, Johne's disease, Mycobacterium avium subsp. paratuberculosis, red deer"@en . "RIV/00027162:_____/06:#0000120!RIV07-MZE-00027162" . "Z(MZE0002716201)" . "8"^^ . . . . . . . "1"^^ . . "Immunological and molecular characterization of susceptibility in relationship to bacterial strain differences in Mycobacterium avium subsp. paratuberculosis infection in the red deer (Cervus elaphus)"@en . "1"^^ . . "[14BDDAC66A63]" . "Immunological and molecular characterization of susceptibility in relationship to bacterial strain differences in Mycobacterium avium subsp. paratuberculosis infection in the red deer (Cervus elaphus)" . "Johneova choroba (JD), vyvolan\u00E1 M.a.paratuberculosis, p\u0159edstavuje v\u00E1\u017En\u00FD probl\u00E9m u farmov\u011B chovan\u00FDch zv\u00ED\u0159at. Zat\u00EDm je toho pom\u011Brn\u011B m\u00E1lo pops\u00E1no o dynamice a imunologick\u00E9 odpov\u011Bdi jelen\u016F trp\u00EDc\u00EDch JD infekc\u00ED. Tato studie prok\u00E1zala typizac\u00ED MAP izol\u00E1t\u016F ze st\u0159evn\u00EDch m\u00EDzn\u00EDch uzlin ze 74 infikovan\u00FDch zv\u00ED\u0159at, \u017Ee klinick\u00E9 MAP izol\u00E1ty z Nov\u00E9ho Z\u00E9landu farmov\u011B chovan\u00FDch jelen\u016F m\u011Bly exkluzivn\u011B bovinn\u00ED genotyp. Citlivost jelen\u016F k MAP byla detailn\u011Bji vy\u0161et\u0159ov\u00E1na experiment\u00E1ln\u011B or\u00E1ln\u00ED infekc\u00ED pou\u017Eit\u00EDm definovan\u00FDch izol\u00E1t\u016F virulentn\u00EDho bovinn\u00EDho a ov\u010D\u00EDho MAP kmene. Or\u00E1ln\u00ED inokuace vysokou nebo st\u0159edn\u00ED d\u00E1vkou bovinn\u00EDho kmene MAP prok\u00E1zala 100% infek\u010Dnost ve srovn\u00E1n\u00ED s 69% vyvol\u00E1n\u00ED infekce n\u00E1sledovan\u00E9 po inokulaci se st\u0159edn\u00ED d\u00E1vkou ov\u010D\u00EDho kmene. Vysok\u00E1 citlivost jelen\u016F k bovinn\u00EDmu kmenu MAP byla konfirmov\u00E1na 50% infek\u010Dnost\u00ED n\u00E1sledovanou po experiment\u00E1ln\u00ED inokulaci s n\u00EDzkou d\u00E1vnou bakteri\u00ED. Tato studie je prvn\u00ED zpr\u00E1vou o experiment\u00E1ln\u00ED MAP infekci jelen\u016F."@cs . "Imunologick\u00E1 a molekul\u00E1rn\u00ED charakteristika rozd\u00EDln\u00E9 citlivosti bakteri\u00E1ln\u00EDch kmen\u016F Mycobacterium avium subsp. paratuberculosis ve vyvol\u00E1n\u00ED infekce u jelen\u016F (Cervus elaphus)"@cs . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . . "478873" . "Infection and Immunity" . "Pavl\u00EDk, Ivo" . "Johne's disease (JD), caused by M.a.paratuberculosis, represents a problem in farmed ruminants. A little is known of the JD infection dynamics or immunological response in deer. In this study, typing of MAP isolates from intestinal lymphatic tissues from 74 infected animals showed that clinical isolates of MAP from New Zealand farmed red deer were exclusively of the bovine strain genotype. The susceptibility of deer to MAP was further investigated by experimental oral-route infection studies using defined isolates of virulent bovine and ovine MAP strains. Oral inoculation with high or medium doses of the bovine strain of MAP established 100% infection rates, compared to 69% infection following inoculation with a medium dose of the ovine strain. The high susceptibility of deer to the bovine strain of MAP was confirmed by a 50% infection rate following experimental inoculation with a low dose of bacteria. This study is the 1st report experimental MAP infection in red deer."@en . "6" . . "0019-9567" . "Immunological and molecular characterization of susceptibility in relationship to bacterial strain differences in Mycobacterium avium subsp. paratuberculosis infection in the red deer (Cervus elaphus)" . "Johne's disease (JD), caused by M.a.paratuberculosis, represents a problem in farmed ruminants. A little is known of the JD infection dynamics or immunological response in deer. In this study, typing of MAP isolates from intestinal lymphatic tissues from 74 infected animals showed that clinical isolates of MAP from New Zealand farmed red deer were exclusively of the bovine strain genotype. The susceptibility of deer to MAP was further investigated by experimental oral-route infection studies using defined isolates of virulent bovine and ovine MAP strains. Oral inoculation with high or medium doses of the bovine strain of MAP established 100% infection rates, compared to 69% infection following inoculation with a medium dose of the ovine strain. The high susceptibility of deer to the bovine strain of MAP was confirmed by a 50% infection rate following experimental inoculation with a low dose of bacteria. This study is the 1st report experimental MAP infection in red deer." . "RIV/00027162:_____/06:#0000120" . . "Imunologick\u00E1 a molekul\u00E1rn\u00ED charakteristika rozd\u00EDln\u00E9 citlivosti bakteri\u00E1ln\u00EDch kmen\u016F Mycobacterium avium subsp. paratuberculosis ve vyvol\u00E1n\u00ED infekce u jelen\u016F (Cervus elaphus)"@cs .