"RIV/00027162:_____/06:#0000058" . . "Most of the scientific papers concerning genetically modified organisms (GMO) are focused on the publication of the detection protocols for GM corn, soyabean, rape or cotton. Minor modifications, such as potatoes, attract little attention. This work was based on developing an easy and cheap PCR method for the detection of the ac2 gene, encoding an antimicrobial peptide from amaranth, which was used for transformation of potatoes. The method was based on duplex PCR which enabled identification of both potato and amaranth specific genes in one tube. The method was enhanced by using positive plasmid controls which were able to reveal presence of inhibitors, reduce the incidence of false negative results, and wrong interpretation during analysis of routine samples. PCR sensitivity using plasmid DNA was determined to be one molecule per reaction. The system was used to find the ac2 gene in 14 variants of potatoes, which were all negative for the inserted ac2 gene from amaranth." . . . "Most of the scientific papers concerning genetically modified organisms (GMO) are focused on the publication of the detection protocols for GM corn, soyabean, rape or cotton. Minor modifications, such as potatoes, attract little attention. This work was based on developing an easy and cheap PCR method for the detection of the ac2 gene, encoding an antimicrobial peptide from amaranth, which was used for transformation of potatoes. The method was based on duplex PCR which enabled identification of both potato and amaranth specific genes in one tube. The method was enhanced by using positive plasmid controls which were able to reveal presence of inhibitors, reduce the incidence of false negative results, and wrong interpretation during analysis of routine samples. PCR sensitivity using plasmid DNA was determined to be one molecule per reaction. The system was used to find the ac2 gene in 14 variants of potatoes, which were all negative for the inserted ac2 gene from amaranth."@en . . "1" . "1" . . "Z(MZE0002716201)" . "Identifikace geneticky modifikovan\u00FDch brambor amplifikac\u00ED genu ac2 z Amaranthus caudatus"@cs . "1"^^ . . "1438-2377" . "1"^^ . . "PCR, detection, GMO, potato, amaranth, antimicrobial peptide"@en . . "A PCR-based method for the detection of genetically modified potatoes by the gene ac2 from Amaranthus caudatus"@en . . . "[FE7D57FBB93F]" . . "P\u0159ibylov\u00E1, Radka" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "European Food Research and Technology" . "A PCR-based method for the detection of genetically modified potatoes by the gene ac2 from Amaranthus caudatus" . . . . "V\u011Bt\u0161ina v\u011Bdeck\u00FDch prac\u00ED na poli geneticky modifikovan\u00FDch organism\u016F (GMO) prezentuje detek\u010Dn\u00ED metody pro identifikaci GM kuku\u0159ice, s\u00F3ji, \u0159epky nebo bavlny. Ostatn\u00EDm modifikacem, jako jsou nap\u0159. brambory, je v\u011Bnov\u00E1na jen men\u0161\u00ED pozornost. C\u00EDlem t\u00E9to pr\u00E1ce bylo vyvinout jednoduchou a levnou PCR metodu pro detekci ac2 genu k\u00F3duj\u00EDc\u00EDho antimikrobn\u00ED peptid z amarantu, kter\u00FD byl pou\u017Eit pro transformaci bramboru. Podstatou metody byla duplex PCR, kter\u00E1 umo\u017E\u0148ovala identifikaci jak ac2 genu, tak specifick\u00E9ho genu bramboru v jedn\u00E9 reakci. Sou\u010D\u00E1st\u00ED metodiky byly tak\u00E9 plazmidov\u00E9 pozitivn\u00ED kontroly, kter\u00E9 slou\u017Eily pro odhalen\u00ED p\u0159\u00EDtomnosti PCR inhibitor\u016F, fale\u0161n\u011B pozitivn\u00EDch v\u00FDsledk\u016F a chybn\u00E9 interperetace v\u00FDsledk\u016F b\u011Bhem rutinn\u00ED anal\u00FDzy. Citlivost metodiky byla testov\u00E1na na plazmidov\u00FDch \u0159ad\u00E1ch a byla stanovena na jednu molekulu na reakci. Syst\u00E9m byl otestov\u00E1n na 14 odr\u016Fd\u00E1ch bramboru, kter\u00E9 byly, pro gen ac2, negativn\u00ED."@cs . "Identifikace geneticky modifikovan\u00FDch brambor amplifikac\u00ED genu ac2 z Amaranthus caudatus"@cs . "RIV/00027162:_____/06:#0000058!RIV07-MZE-00027162" . . "A PCR-based method for the detection of genetically modified potatoes by the gene ac2 from Amaranthus caudatus"@en . . "463754" . "4"^^ . . "A PCR-based method for the detection of genetically modified potatoes by the gene ac2 from Amaranthus caudatus" . .