"Z(MSM6215712402), Z(MZE0002716201)" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "0090-3558" . "Pavl\u00EDk, Ivo" . "3" . "Detekce Mycobacterium avium subsp. paratuberculosis u dvou medv\u011Bd\u016F hn\u011Bd\u00FDch ve st\u0159edn\u00EDch evropsk\u00FDch Karpatech"@cs . "V\u00FDskyt mykobakteri\u00E1ln\u00EDch infekc\u00ED u medv\u011Bda hn\u011Bd\u00E9ho byl sledov\u00E1na v N\u00E1rodn\u00EDm parku N\u00EDzk\u00E9 Tatry na Slovensku. 20 vzork\u016F tk\u00E1n\u011B medv\u011Bda hn\u011Bd\u00E9ho bylo vy\u0161et\u0159eno mikroskopicky a kultiva\u010Dn\u011B na p\u0159\u00EDtomnost mykobakteri\u00ED. AFR byly zji\u0161t\u011Bny barven\u00ED dle Ziehl-Neelsena z otisku vzorku ledviny jednoho medv\u011Bda a\u010Dkoliv kultiva\u010Dn\u00ED vy\u0161et\u0159en\u00ED bylo negativn\u00ED. M.a.paratuberculosis (MAP), p\u016Fvodce paratuberkul\u00F3zy u p\u0159e\u017Ev\u00FDkavc\u016F, byl izolov\u00E1n z intestinaln\u00ED muk\u00F3zy dal\u0161\u00EDch dvou medv\u011Bd\u016F hn\u011Bd\u00FDch. Izol\u00E1ty byly identifikov\u00E1ny metodou IS900-PCR. Standardizovanou IS900-RFLP anal\u00FDzou byly MAP izol\u00E1ty ur\u010Deny jako RFLP typ B-C1, stejn\u00FD RFLP typ byl detekov\u00E1n u infikovan\u00E9ho skotu v p\u0159ilehl\u00E9 pasteveck\u00E9 oblasti. Tato pr\u00E1ce poprv\u00E9 popisuje izolaci MAP z medv\u011Bda hn\u011Bd\u00E9ho. N\u00E1mi z\u00EDskan\u00E9 v\u00FDsledky potvrzuj\u00ED, \u017Ee MAP m\u016F\u017Ee infikovat i jin\u00E9 zv\u00ED\u0159ata ne\u017E p\u0159e\u017Ev\u00FDkavce, kter\u00E9 pak mohou sehr\u00E1t roli jako potenci\u00E1ln\u00ED vektor a/nebo rezervo\u00E1r infekce v voln\u00E9 p\u0159\u00EDrod\u011B."@cs . . "epidemiology, Johne's disease, carnivores, wildlife"@en . . "Detection of Mycobacterium avium subsp. paratuberculosis in two brown bears in the central European Carpathians" . . "3" . . . "RIV/00027162:_____/06:#0000056" . . "The incidence of mycobacterial infections was monitored in brown bears in the National Park Low Tatras in Slovakia. Tissue samples of 20 brown bears were examined microscopically and by culture for the presence of mycobacteria. AFR were detected by Ziehl-Neelsen staining in a smear from the kidney of one brown bear, although the culture was negative for mycobacteria. M.a.paratuberculosis (MAP), the causative agent of paratuberculosis in ruminants, was isolated from the intestinal mucosa of another two brown bears. The isolates were identified by PCR for the specific insertion sequence IS900. Using standardized IS900 RFLP analysis, the MAP isolates were classified as RFLP type B-C1, which also were detected in the infected cattle in surrounding area. This study describes the first isolation of MAP from a brown bear. Our results confirm that animal species other than ruminants can become infected with MAP and can act as potential vectors and/or reservoirs of the infection."@en . . . "The incidence of mycobacterial infections was monitored in brown bears in the National Park Low Tatras in Slovakia. Tissue samples of 20 brown bears were examined microscopically and by culture for the presence of mycobacteria. AFR were detected by Ziehl-Neelsen staining in a smear from the kidney of one brown bear, although the culture was negative for mycobacteria. M.a.paratuberculosis (MAP), the causative agent of paratuberculosis in ruminants, was isolated from the intestinal mucosa of another two brown bears. The isolates were identified by PCR for the specific insertion sequence IS900. Using standardized IS900 RFLP analysis, the MAP isolates were classified as RFLP type B-C1, which also were detected in the infected cattle in surrounding area. This study describes the first isolation of MAP from a brown bear. Our results confirm that animal species other than ruminants can become infected with MAP and can act as potential vectors and/or reservoirs of the infection." . "Detection of Mycobacterium avium subsp. paratuberculosis in two brown bears in the central European Carpathians"@en . "Detection of Mycobacterium avium subsp. paratuberculosis in two brown bears in the central European Carpathians"@en . "Journal of Wildlife Diseases" . . "1"^^ . "5"^^ . "471027" . . "1"^^ . . "[32EBA14CB36A]" . "Detekce Mycobacterium avium subsp. paratuberculosis u dvou medv\u011Bd\u016F hn\u011Bd\u00FDch ve st\u0159edn\u00EDch evropsk\u00FDch Karpatech"@cs . "Detection of Mycobacterium avium subsp. paratuberculosis in two brown bears in the central European Carpathians" . . . . . "RIV/00027162:_____/06:#0000056!RIV07-MZE-00027162" . .