"1"^^ . "1"^^ . "Salmonella typhimurium; Integron; Retron; IS6100"@en . "3-4" . "[C48081403B2E]" . "541012" . "Retron reverse transcriptase (rrtT) can be lost in multidrug resistant Salmonella enterica serovar Typhimurium DT 104 strains and influences virulence for mice" . . "Veterinary Microbiology" . . . . "111" . . "In Salmonella enterica serovar Typhimurium, rrtT retron reverse transcriptase, which is part of St85 retron, is quite ubiquitous and is located in the thdF - yidY intergenic region. In this study we show that rrtT is relatively unstable in multidrugresistant, Salmonella genomic island 1 positive strains. Out of 365 field strains, 55 were free of retron. We propose that intramolecular transposition of IS6100 followed by homologous recombination was responsible for the excision. Excision of retrontogetherwith the right end of SGI1 may also result in its stabilization in the S. typhimurium genome. Experimental deletion of rrtT resulted in an accelerated course of infection in orally infected mice. Since the retron excision occurs exclusively inmultidrugresistant S. typhimurium, it cannot be excluded that such strains may increase their virulence in the future." . . "7"^^ . . . . . "RIV/00027162:_____/05:06000018" . . . "191;197" . "rrtT retron reversn\u00ED transcript\u00E1za je nestabiln\u00ED v multirezistentn\u00EDch kmenech Salmonella enterica serovar Typhimurium"@cs . . "RIV/00027162:_____/05:06000018!RIV06-MZE-00027162" . "Retron reverse transcriptase (rrtT) can be lost in multidrug resistant Salmonella enterica serovar Typhimurium DT 104 strains and influences virulence for mice"@en . "U kmen\u016F Salmonella enterica serovar Typhimurium nach\u00E1z\u00EDme \u010Dasto retron reverzn\u00ED transkript\u00E1zu (rrtT). Ta je sou\u010D\u00E1st\u00ED retronu St85, kter\u00FD je lokalizov\u00E1n v oblasti mezi geny thdF - yidY. V t\u00E9to studii jsme zjistili, \u017Ee u multirezistentn\u00EDch kmen\u016F S.Typhimurium, kter\u00E9 nesou v genomu Salmonella genomic island 1, je retron nestabiln\u00ED. Popsali jsme zp\u016Fsob intramolekul\u00E1rn\u00ED transpozice IS6100, n\u00E1sledovan\u00E9 homologn\u00ED rekombinac\u00ED, kter\u00E1 by mohla v\u00E9st k deleci retronu. Tato delece retronu spolu s prav\u00FDmkoncem SGI1 zp\u016Fsobuje mimo jin\u00E9 stabilizaci SGI 1 v genomu. Pozorovali jsme zrychlen\u00FD pr\u016Fb\u011Bh infekce u my\u0161\u00ED, infikovan\u00FDch kmenem se z\u00E1m\u011Brnou delec\u00ED rrtT. Proto\u017Ee se tato delece p\u0159irozen\u011B vyskytuje jen u multirezistentn\u00EDch kmen\u016F, ned\u00E1 se vylou\u010Dit, \u017Ee tytokmeny mohou d\u00EDky t\u00E9to deleci v budoucnosti zv\u00FD\u0161it svoj\u00ED virulenci."@cs . . . "P(1B44019), Z(MZE0002716201)" . "Rychl\u00EDk, Ivan" . . . "NL - Nizozemsko" . "0378-1135" . "Retron reverse transcriptase (rrtT) can be lost in multidrug resistant Salmonella enterica serovar Typhimurium DT 104 strains and influences virulence for mice" . . "Retron reverse transcriptase (rrtT) can be lost in multidrug resistant Salmonella enterica serovar Typhimurium DT 104 strains and influences virulence for mice"@en . "In Salmonella enterica serovar Typhimurium, rrtT retron reverse transcriptase, which is part of St85 retron, is quite ubiquitous and is located in the thdF - yidY intergenic region. In this study we show that rrtT is relatively unstable in multidrugresistant, Salmonella genomic island 1 positive strains. Out of 365 field strains, 55 were free of retron. We propose that intramolecular transposition of IS6100 followed by homologous recombination was responsible for the excision. Excision of retrontogetherwith the right end of SGI1 may also result in its stabilization in the S. typhimurium genome. Experimental deletion of rrtT resulted in an accelerated course of infection in orally infected mice. Since the retron excision occurs exclusively inmultidrugresistant S. typhimurium, it cannot be excluded that such strains may increase their virulence in the future."@en . "rrtT retron reversn\u00ED transcript\u00E1za je nestabiln\u00ED v multirezistentn\u00EDch kmenech Salmonella enterica serovar Typhimurium"@cs .